Restriction map for
pF1KB5754
(Linear) (Six-base) MAPSORT of: KIBB5754.seq Check: 6675 from: 1 to: 2247
KIBB5754.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1870 1923 2247
Size: 1870 53 324
Fragments arranged by size:
1870 324 53
AhdI GACnn_n'nnGTC
Cuts at: 0 862 2247
Size: 862 1385
AleI CACnn'nnGTG
Cuts at: 0 85 2247
Size: 85 2162
AlwNI CAG_nnn'CTG
Cuts at: 0 1582 2247
Size: 1582 665
ApoI r'AATT_y
Cuts at: 0 226 647 1042 1111 1588 2247
Size: 226 421 395 69 477 659
Fragments arranged by size:
659 477 421 395 226 69
AseI AT'TA_AT
Cuts at: 0 752 2247
Size: 752 1495
BamHI G'GATC_C
Cuts at: 0 237 2247
Size: 237 2010
BanII G_rGCy'C
Cuts at: 0 1655 2247
Size: 1655 592
BbsI GAAGACnn'nnnn_
Cuts at: 0 469 985 2163 2247
Size: 469 516 1178 84
Fragments arranged by size:
1178 516 469 84
BciVI GTATCCnnnnn_n'
Cuts at: 0 1682 2247
Size: 1682 565
BclI T'GATC_A
Cuts at: 0 453 2247
Size: 453 1794
BmgBI CAC'GTC
Cuts at: 0 1453 2247
Size: 1453 794
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 1940 2247
Size: 107 1833 307
Fragments arranged by size:
1833 307 107
BsaI GGTCTCn'nnnn_
Cuts at: 0 211 1680 2247
Size: 211 1469 567
Fragments arranged by size:
1469 567 211
BsaAI yAC'GTr
Cuts at: 0 76 553 2247
Size: 76 477 1694
Fragments arranged by size:
1694 477 76
BsaBI GATnn'nnATC
Cuts at: 0 242 2247
Size: 242 2005
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 58 253 283 2247
Size: 28 30 195 30 1964
Fragments arranged by size:
1964 195 30 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 760 2247
Size: 760 1487
BseYI C'CCAG_C
Cuts at: 0 1456 1479 2247
Size: 1456 23 768
Fragments arranged by size:
1456 768 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 533 947 2247
Size: 533 414 1300
Fragments arranged by size:
1300 533 414
BsiHKAI G_wGCw'C
Cuts at: 0 41 2247
Size: 41 2206
BsmI GAATG_Cn'
Cuts at: 0 543 2065 2247
Size: 543 1522 182
Fragments arranged by size:
1522 543 182
Bsp1286I G_dGCh'C
Cuts at: 0 41 1655 2247
Size: 41 1614 592
Fragments arranged by size:
1614 592 41
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1673 2247
Size: 1673 574
BstYI r'GATC_y
Cuts at: 0 12 126 237 1578 2247
Size: 12 114 111 1341 669
Fragments arranged by size:
1341 669 114 111 12
BtgI C'CryG_G
Cuts at: 0 602 2132 2247
Size: 602 1530 115
Fragments arranged by size:
1530 602 115
BtsI GCAGTG_nn'
Cuts at: 0 1188 2247
Size: 1188 1059
DraI TTT'AAA
Cuts at: 0 1869 2247
Size: 1869 378
EaeI y'GGCC_r
Cuts at: 0 1925 2247
Size: 1925 322
EarI CTCTTCn'nnn_
Cuts at: 0 166 1976 2247
Size: 166 1810 271
Fragments arranged by size:
1810 271 166
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 1870 1923 1940 2247
Size: 107 1763 53 17 307
Fragments arranged by size:
1763 307 107 53 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1694 2247
Size: 1694 553
EcoO109I rG'GnC_Cy
Cuts at: 0 98 2247
Size: 98 2149
EcoRI G'AATT_C
Cuts at: 0 647 2247
Size: 647 1600
EcoRV GAT'ATC
Cuts at: 0 879 2247
Size: 879 1368
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 117 531 1824 2247
Size: 117 414 1293 423
Fragments arranged by size:
1293 423 414 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 848 880 1303 1335 2008 2040 2247
Size: 848 32 423 32 673 32 207
Fragments arranged by size:
848 673 423 207 32 32 32
HincII GTy'rAC
Cuts at: 0 704 2247
Size: 704 1543
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1369 2247
Size: 1369 878
MscI TGG'CCA
Cuts at: 0 1927 2247
Size: 1927 320
MslI CAynn'nnrTG
Cuts at: 0 31 85 385 2116 2247
Size: 31 54 300 1731 131
Fragments arranged by size:
1731 300 131 54 31
MspA1I CmG'CkG
Cuts at: 0 1570 2247
Size: 1570 677
NcoI C'CATG_G
Cuts at: 0 602 2132 2247
Size: 602 1530 115
Fragments arranged by size:
1530 602 115
NspI r_CATG'y
Cuts at: 0 114 2247
Size: 114 2133
PmlI CAC'GTG
Cuts at: 0 553 2247
Size: 553 1694
PshAI GACnn'nnGTC
Cuts at: 0 1748 2247
Size: 1748 499
PvuII CAG'CTG
Cuts at: 0 1570 2247
Size: 1570 677
SexAI A'CCwGG_T
Cuts at: 0 691 2247
Size: 691 1556
SfcI C'TryA_G
Cuts at: 0 1571 2247
Size: 1571 676
SspI AAT'ATT
Cuts at: 0 1269 2247
Size: 1269 978
StyI C'CwwG_G
Cuts at: 0 602 1412 1966 2132 2247
Size: 602 810 554 166 115
Fragments arranged by size:
810 602 554 166 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1159 2247
Size: 1159 1088
TatI w'GTAC_w
Cuts at: 0 1946 2247
Size: 1946 301
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 31 63 2247
Size: 31 32 2184
Fragments arranged by size:
2184 32 31
XbaI T'CTAG_A
Cuts at: 0 393 2247
Size: 393 1854
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 288 2247
Size: 288 1959
XmnI GAAnn'nnTTC
Cuts at: 0 389 466 2247
Size: 389 77 1781
Fragments arranged by size:
1781 389 77
Enzymes that do cut:
AcuI AhdI AleI AlwNI ApoI AseI BamHI BanII
BbsI BciVI BclI BmgBI BpmI BsaI BsaAI BsaBI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BstYI BtgI BtsI DraI EaeI EarI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI Hin4I HincII MmeI MscI
MslI MspA1I NcoI NspI PmlI PshAI PvuII SexAI
SfcI SspI StyI TaqII TatI TstI XbaI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanI BbeI BbvCI BcgI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI Bpu10I BpuEI
BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoICRI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PpiI PpuMI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XhoI XmaI ZraI