Restriction map for
pF1KB5747
(Linear) (Six-base) MAPSORT of: KIBB5747.seq Check: 5482 from: 1 to: 2421
KIBB5747.orf
With 182 enzymes: SgfI *
March 2, 2010 14:26 ..
AccI GT'mk_AC
Cuts at: 0 845 2421
Size: 845 1576
Acc65I G'GTAC_C
Cuts at: 0 1630 2421
Size: 1630 791
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 750 1210 1463 2236 2421
Size: 106 644 460 253 773 185
Fragments arranged by size:
773 644 460 253 185 106
AfeI AGC'GCT
Cuts at: 0 421 2421
Size: 421 2000
AleI CACnn'nnGTG
Cuts at: 0 680 2421
Size: 680 1741
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1668 1700 2421
Size: 1668 32 721
Fragments arranged by size:
1668 721 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2214 2320 2421
Size: 2214 106 101
Fragments arranged by size:
2214 106 101
ApoI r'AATT_y
Cuts at: 0 14 79 454 1758 1897 1990 2190 2258
Size: 14 65 375 1304 139 93 200 68
Cuts at: 2258 2352 2421
Size: 94 69
Fragments arranged by size:
1304 375 200 139 94 93 69 68
65 14
BanI G'GyrC_C
Cuts at: 0 1323 1630 2421
Size: 1323 307 791
Fragments arranged by size:
1323 791 307
BanII G_rGCy'C
Cuts at: 0 623 1255 2421
Size: 623 632 1166
Fragments arranged by size:
1166 632 623
BbsI GAAGACnn'nnnn_
Cuts at: 0 2224 2421
Size: 2224 197
BbvCI CC'TCA_GC
Cuts at: 0 1929 2421
Size: 1929 492
BciVI GTATCCnnnnn_n'
Cuts at: 0 483 2421
Size: 483 1938
BfrBI ATG'CAT
Cuts at: 0 386 671 1470 2421
Size: 386 285 799 951
Fragments arranged by size:
951 799 386 285
BlpI GC'TnA_GC
Cuts at: 0 431 2421
Size: 431 1990
BmrI ACTGGGnnnn_n'
Cuts at: 0 435 2421
Size: 435 1986
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1162 1913 2322 2421
Size: 360 802 751 409 99
Fragments arranged by size:
802 751 409 360 99
Bpu10I CC'TnA_GC
Cuts at: 0 1929 2421
Size: 1929 492
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 2421
Size: 637 1784
BsaAI yAC'GTr
Cuts at: 0 1528 2421
Size: 1528 893
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2072 2102 2235 2265 2421
Size: 2072 30 133 30 156
Fragments arranged by size:
2072 156 133 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 2075 2421
Size: 79 1996 346
Fragments arranged by size:
1996 346 79
BsiHKAI G_wGCw'C
Cuts at: 0 623 1566 2421
Size: 623 943 855
Fragments arranged by size:
943 855 623
BsmI GAATG_Cn'
Cuts at: 0 671 2421
Size: 671 1750
Bsp1286I G_dGCh'C
Cuts at: 0 623 1255 1566 2421
Size: 623 632 311 855
Fragments arranged by size:
855 632 623 311
BstAPI GCAn_nnn'nTGC
Cuts at: 0 677 2421
Size: 677 1744
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1386 2411 2421
Size: 1386 1025 10
Fragments arranged by size:
1386 1025 10
BtgI C'CryG_G
Cuts at: 0 2183 2421
Size: 2183 238
BtsI GCAGTG_nn'
Cuts at: 0 675 687 1498 2421
Size: 675 12 811 923
Fragments arranged by size:
923 811 675 12
EaeI y'GGCC_r
Cuts at: 0 577 2421
Size: 577 1844
EarI CTCTTCn'nnn_
Cuts at: 0 1088 1229 1726 2421
Size: 1088 141 497 695
Fragments arranged by size:
1088 695 497 141
EcoICRI GAG'CTC
Cuts at: 0 621 2421
Size: 621 1800
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 360 750 1162 1210 1463 1913 2236
Size: 106 254 390 412 48 253 450 323
Cuts at: 2236 2322 2421
Size: 86 99
Fragments arranged by size:
450 412 390 323 254 253 106 99
86 48
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 2421
Size: 34 2387
EcoO109I rG'GnC_Cy
Cuts at: 0 310 2421
Size: 310 2111
EcoRI G'AATT_C
Cuts at: 0 1758 1897 2421
Size: 1758 139 524
Fragments arranged by size:
1758 524 139
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1145 1419 2421
Size: 1145 274 1002
Fragments arranged by size:
1145 1002 274
HaeII r_GCGC'y
Cuts at: 0 423 2421
Size: 423 1998
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1237 1269 1440 1472 1735 1767 2421
Size: 1237 32 171 32 263 32 654
Fragments arranged by size:
1237 654 263 171 32 32 32
KpnI G_GTAC'C
Cuts at: 0 1634 2421
Size: 1634 787
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1789 2421
Size: 1789 632
MscI TGG'CCA
Cuts at: 0 579 2421
Size: 579 1842
MslI CAynn'nnrTG
Cuts at: 0 376 680 1180 1962 2421
Size: 376 304 500 782 459
Fragments arranged by size:
782 500 459 376 304
MspA1I CmG'CkG
Cuts at: 0 450 1801 1933 2421
Size: 450 1351 132 488
Fragments arranged by size:
1351 488 450 132
NcoI C'CATG_G
Cuts at: 0 2183 2421
Size: 2183 238
NdeI CA'TA_TG
Cuts at: 0 667 1506 2421
Size: 667 839 915
Fragments arranged by size:
915 839 667
NsiI A_TGCA'T
Cuts at: 0 388 673 1472 2421
Size: 388 285 799 949
Fragments arranged by size:
949 799 388 285
NspI r_CATG'y
Cuts at: 0 386 1474 2421
Size: 386 1088 947
Fragments arranged by size:
1088 947 386
PasI CC'CwG_GG
Cuts at: 0 306 2421
Size: 306 2115
PflMI CCAn_nnn'nTGG
Cuts at: 0 1187 1213 2421
Size: 1187 26 1208
Fragments arranged by size:
1208 1187 26
PfoI T'CCnGG_A
Cuts at: 0 29 329 785 2223 2421
Size: 29 300 456 1438 198
Fragments arranged by size:
1438 456 300 198 29
PpuMI rG'GwC_Cy
Cuts at: 0 310 2421
Size: 310 2111
PstI C_TGCA'G
Cuts at: 0 23 682 2316 2421
Size: 23 659 1634 105
Fragments arranged by size:
1634 659 105 23
PvuII CAG'CTG
Cuts at: 0 450 1801 1933 2421
Size: 450 1351 132 488
Fragments arranged by size:
1351 488 450 132
SacI G_AGCT'C
Cuts at: 0 623 2421
Size: 623 1798
SapI GCTCTTCn'nnn_
Cuts at: 0 1088 2421
Size: 1088 1333
SfcI C'TryA_G
Cuts at: 0 19 602 678 2150 2312 2421
Size: 19 583 76 1472 162 109
Fragments arranged by size:
1472 583 162 109 76 19
SmlI C'TyrA_G
Cuts at: 0 616 2421
Size: 616 1805
SnaBI TAC'GTA
Cuts at: 0 1528 2421
Size: 1528 893
SphI G_CATG'C
Cuts at: 0 386 2421
Size: 386 2035
SspI AAT'ATT
Cuts at: 0 1773 2418 2421
Size: 1773 645 3
Fragments arranged by size:
1773 645 3
StuI AGG'CCT
Cuts at: 0 807 2421
Size: 807 1614
StyI C'CwwG_G
Cuts at: 0 287 1379 1819 2183 2392 2421
Size: 287 1092 440 364 209 29
Fragments arranged by size:
1092 440 364 287 209 29
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 319 2421
Size: 319 2102
TatI w'GTAC_w
Cuts at: 0 110 1803 2421
Size: 110 1693 618
Fragments arranged by size:
1693 618 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 294 326 2072 2104 2421
Size: 294 32 1746 32 317
Fragments arranged by size:
1746 317 294 32 32
XbaI T'CTAG_A
Cuts at: 0 2103 2421
Size: 2103 318
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2408 2421
Size: 2408 13
XmnI GAAnn'nnTTC
Cuts at: 0 2321 2421
Size: 2321 100
Enzymes that do cut:
AccI Acc65I AcuI AfeI AleI AloI AlwNI ApoI
BanI BanII BbsI BbvCI BciVI BfrBI BlpI BmrI
BpmI Bpu10I BpuEI BsaAI BsaXI BseRI BsiHKAI BsmI
Bsp1286I BstAPI BstXI BtgI BtsI EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PasI PflMI PfoI PpuMI PstI PvuII SacI SapI
SfcI SmlI SnaBI SphI SspI StuI StyI TaqII
TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AhdI AlfI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BcgI BclI BglI BglII Bme1580I BmgBI
BmtI BplI BsaI BsaBI BsaHI BsaWI BseYI BsgI
BsiEI BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoRV FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SpeI SrfI SwaI Tth111I XhoI XmaI ZraI