Restriction map for
pF1KB5716
(Linear) (Six-base) MAPSORT of: KIBB5716.seq Check: 3612 from: 1 to: 2163
KIBB5716.orf
With 182 enzymes: SgfI *
October 7, 2009 14:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 423 2163
Size: 423 1740
AccI GT'mk_AC
Cuts at: 0 1848 2163
Size: 1848 315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 556 1012 1399 1424 2163
Size: 277 279 456 387 25 739
Fragments arranged by size:
739 456 387 279 277 25
AfeI AGC'GCT
Cuts at: 0 25 2163
Size: 25 2138
AflIII A'CryG_T
Cuts at: 0 2061 2102 2163
Size: 2061 41 61
Fragments arranged by size:
2061 61 41
AgeI A'CCGG_T
Cuts at: 0 982 2163
Size: 982 1181
AhdI GACnn_n'nnGTC
Cuts at: 0 1505 2163
Size: 1505 658
AleI CACnn'nnGTG
Cuts at: 0 1142 2163
Size: 1142 1021
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 940 1160 2163
Size: 940 220 1003
Fragments arranged by size:
1003 940 220
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 514 546 2163
Size: 514 32 1617
Fragments arranged by size:
1617 514 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1058 1134 2163
Size: 1058 76 1029
Fragments arranged by size:
1058 1029 76
ApaLI G'TGCA_C
Cuts at: 0 715 2163
Size: 715 1448
AvaI C'yCGr_G
Cuts at: 0 1495 1759 2163
Size: 1495 264 404
Fragments arranged by size:
1495 404 264
BanI G'GyrC_C
Cuts at: 0 176 736 979 1185 2163
Size: 176 560 243 206 978
Fragments arranged by size:
978 560 243 206 176
BanII G_rGCy'C
Cuts at: 0 1979 2072 2163
Size: 1979 93 91
Fragments arranged by size:
1979 93 91
BbeI G_GCGC'C
Cuts at: 0 180 2163
Size: 180 1983
BbsI GAAGACnn'nnnn_
Cuts at: 0 985 1838 2163
Size: 985 853 325
Fragments arranged by size:
985 853 325
BbvCI CC'TCA_GC
Cuts at: 0 166 661 969 2163
Size: 166 495 308 1194
Fragments arranged by size:
1194 495 308 166
BciVI GTATCCnnnnn_n'
Cuts at: 0 1409 2163
Size: 1409 754
BglI GCCn_nnn'nGGC
Cuts at: 0 1049 2163
Size: 1049 1114
BglII A'GATC_T
Cuts at: 0 1565 2163
Size: 1565 598
BlpI GC'TnA_GC
Cuts at: 0 548 676 2163
Size: 548 128 1487
Fragments arranged by size:
1487 548 128
Bme1580I G_kGCm'C
Cuts at: 0 719 839 982 2163
Size: 719 120 143 1181
Fragments arranged by size:
1181 719 143 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 381 1484 2163
Size: 381 1103 679
Fragments arranged by size:
1103 679 381
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 305 1321 1453 1689 1903 2056 2163
Size: 215 90 1016 132 236 214 153 107
Fragments arranged by size:
1016 236 215 214 153 132 107 90
Bpu10I CC'TnA_GC
Cuts at: 0 166 661 969 1813 1826 2163
Size: 166 495 308 844 13 337
Fragments arranged by size:
844 495 337 308 166 13
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 2163
Size: 739 1424
BsaI GGTCTCn'nnnn_
Cuts at: 0 462 586 1415 2163
Size: 462 124 829 748
Fragments arranged by size:
829 748 462 124
BsaAI yAC'GTr
Cuts at: 0 720 2163
Size: 720 1443
BsaBI GATnn'nnATC
Cuts at: 0 1960 2163
Size: 1960 203
BsaHI Gr'CG_yC
Cuts at: 0 177 990 2163
Size: 177 813 1173
Fragments arranged by size:
1173 813 177
BsaWI w'CCGG_w
Cuts at: 0 982 2163
Size: 982 1181
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 580 1148 1178 2163
Size: 550 30 568 30 985
Fragments arranged by size:
985 568 550 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 204 388 466 1033 2007 2163
Size: 160 44 184 78 567 974 156
Fragments arranged by size:
974 567 184 160 156 78 44
BseYI C'CCAG_C
Cuts at: 0 1215 1227 1534 2163
Size: 1215 12 307 629
Fragments arranged by size:
1215 629 307 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 383 1120 1630 1666 2163
Size: 260 123 737 510 36 497
Fragments arranged by size:
737 510 497 260 123 36
BsiEI CG_ry'CG
Cuts at: 0 864 2163
Size: 864 1299
BsiHKAI G_wGCw'C
Cuts at: 0 152 719 1149 2163
Size: 152 567 430 1014
Fragments arranged by size:
1014 567 430 152
Bsp1286I G_dGCh'C
Cuts at: 0 152 719 839 982 1149 1979 2072 2163
Size: 152 567 120 143 167 830 93 91
Fragments arranged by size:
830 567 167 152 143 120 93 91
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 423 444 681 1526 2163
Size: 423 21 237 845 637
Fragments arranged by size:
845 637 423 237 21
BsrDI GCAATG_nn'
Cuts at: 0 1835 2163
Size: 1835 328
BsrFI r'CCGG_y
Cuts at: 0 711 859 982 1188 2163
Size: 711 148 123 206 975
Fragments arranged by size:
975 711 206 148 123
BsrGI T'GTAC_A
Cuts at: 0 1635 2163
Size: 1635 528
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1134 2163
Size: 1134 1029
BstEII G'GTnAC_C
Cuts at: 0 954 2163
Size: 954 1209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1356 2163
Size: 1356 807
BstYI r'GATC_y
Cuts at: 0 1061 1565 2163
Size: 1061 504 598
Fragments arranged by size:
1061 598 504
BtgI C'CryG_G
Cuts at: 0 1787 1940 2138 2163
Size: 1787 153 198 25
Fragments arranged by size:
1787 198 153 25
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 942 2163
Size: 942 1221
BtsI GCAGTG_nn'
Cuts at: 0 272 371 493 856 1238 1368 1613 2163
Size: 272 99 122 363 382 130 245 550
Fragments arranged by size:
550 382 363 272 245 130 122 99
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2045 2080 2163
Size: 2045 35 83
Fragments arranged by size:
2045 83 35
DraIII CAC_nnn'GTG
Cuts at: 0 1082 2033 2163
Size: 1082 951 130
Fragments arranged by size:
1082 951 130
EaeI y'GGCC_r
Cuts at: 0 432 633 861 885 2163
Size: 432 201 228 24 1278
Fragments arranged by size:
1278 432 228 201 24
EagI C'GGCC_G
Cuts at: 0 861 2163
Size: 861 1302
EarI CTCTTCn'nnn_
Cuts at: 0 1418 2163
Size: 1418 745
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 215 277 305 556 1012 1321 1399 1424
Size: 215 62 28 251 456 309 78 25
Cuts at: 1424 1453 1689 1903 2056 2163
Size: 29 236 214 153 107
Fragments arranged by size:
456 309 251 236 215 214 153 107
78 62 29 28 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 259 1154 2163
Size: 259 895 1009
Fragments arranged by size:
1009 895 259
EcoO109I rG'GnC_Cy
Cuts at: 0 162 311 768 806 1172 2163
Size: 162 149 457 38 366 991
Fragments arranged by size:
991 457 366 162 149 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1429 2163
Size: 1429 734
HaeII r_GCGC'y
Cuts at: 0 27 180 825 2163
Size: 27 153 645 1338
Fragments arranged by size:
1338 645 153 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 373 405 1040 1072 1148 1180 1491 1523
Size: 373 32 635 32 76 32 311 32
Cuts at: 1523 2163
Size: 640
Fragments arranged by size:
640 635 373 311 76 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1307 2163
Size: 1307 856
KasI G'GCGC_C
Cuts at: 0 176 2163
Size: 176 1987
MfeI C'AATT_G
Cuts at: 0 1619 2163
Size: 1619 544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1340 1832 2163
Size: 1340 492 331
Fragments arranged by size:
1340 492 331
MscI TGG'CCA
Cuts at: 0 434 635 887 2163
Size: 434 201 252 1276
Fragments arranged by size:
1276 434 252 201
MslI CAynn'nnrTG
Cuts at: 0 403 1142 1786 1867 2163
Size: 403 739 644 81 296
Fragments arranged by size:
739 644 403 296 81
MspA1I CmG'CkG
Cuts at: 0 601 1370 1614 2163
Size: 601 769 244 549
Fragments arranged by size:
769 601 549 244
NarI GG'CG_CC
Cuts at: 0 177 2163
Size: 177 1986
NcoI C'CATG_G
Cuts at: 0 1787 1940 2163
Size: 1787 153 223
Fragments arranged by size:
1787 223 153
NspI r_CATG'y
Cuts at: 0 2065 2106 2163
Size: 2065 41 57
Fragments arranged by size:
2065 57 41
PasI CC'CwG_GG
Cuts at: 0 102 1110 2163
Size: 102 1008 1053
Fragments arranged by size:
1053 1008 102
PciI A'CATG_T
Cuts at: 0 2061 2102 2163
Size: 2061 41 61
Fragments arranged by size:
2061 61 41
PflMI CCAn_nnn'nTGG
Cuts at: 0 1667 2163
Size: 1667 496
PfoI T'CCnGG_A
Cuts at: 0 391 2163
Size: 391 1772
PmlI CAC'GTG
Cuts at: 0 720 2163
Size: 720 1443
PpuMI rG'GwC_Cy
Cuts at: 0 311 768 1172 2163
Size: 311 457 404 991
Fragments arranged by size:
991 457 404 311
PstI C_TGCA'G
Cuts at: 0 455 2163
Size: 455 1708
PvuII CAG'CTG
Cuts at: 0 601 1614 2163
Size: 601 1013 549
Fragments arranged by size:
1013 601 549
ScaI AGT'ACT
Cuts at: 0 562 2163
Size: 562 1601
SfcI C'TryA_G
Cuts at: 0 451 1998 2163
Size: 451 1547 165
Fragments arranged by size:
1547 451 165
SfoI GGC'GCC
Cuts at: 0 178 2163
Size: 178 1985
SgrAI Cr'CCGG_yG
Cuts at: 0 982 2163
Size: 982 1181
SmlI C'TyrA_G
Cuts at: 0 754 1495 2163
Size: 754 741 668
Fragments arranged by size:
754 741 668
StuI AGG'CCT
Cuts at: 0 892 2163
Size: 892 1271
StyI C'CwwG_G
Cuts at: 0 158 1349 1787 1940 2163
Size: 158 1191 438 153 223
Fragments arranged by size:
1191 438 223 158 153
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 362 724 811 885 1154 1548 2163
Size: 362 362 87 74 269 394 615
Fragments arranged by size:
615 394 362 362 269 87 74
TatI w'GTAC_w
Cuts at: 0 218 560 1635 2163
Size: 218 342 1075 528
Fragments arranged by size:
1075 528 342 218
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 337 369 1269 1301 1789 1821 2163
Size: 337 32 900 32 488 32 342
Fragments arranged by size:
900 488 342 337 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 424 2163
Size: 424 1739
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1117 2163
Size: 1117 1046
XhoI C'TCGA_G
Cuts at: 0 1495 2163
Size: 1495 668
XmnI GAAnn'nnTTC
Cuts at: 0 1768 2163
Size: 1768 395
Enzymes that do cut:
AarI AccI AcuI AfeI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AvaI BanI BanII BbeI
BbsI BbvCI BciVI BglI BglII BlpI Bme1580I BmrI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I HincII KasI MfeI
MmeI MscI MslI MspA1I NarI NcoI NspI PasI
PciI PflMI PfoI PmlI PpuMI PstI PvuII ScaI
SfcI SfoI SgrAI SmlI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BcgI BclI BfrBI BmgBI
BmtI BplI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BssHII BssSI BstBI BstZ17I Bsu36I ClaI DraI DrdI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HindIII
HpaI KpnI MluI NaeI NdeI NgoMIV NheI NotI
NruI NsiI PacI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SgfI SmaI SnaBI SpeI
SphI SrfI SspI SwaI XbaI XmaI ZraI