Restriction map for
pF1KB5708
(Linear) (Six-base) MAPSORT of: KIBB5708.seq Check: 2373 from: 1 to: 2154
KIBB5708.orf
With 182 enzymes: SgfI *
April 6, 2017 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1251 2154
Size: 1251 903
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1104 1570 1757 1777 1883 2137 2154
Size: 634 470 466 187 20 106 254 17
Fragments arranged by size:
634 470 466 254 187 106 20 17
AflIII A'CryG_T
Cuts at: 0 1334 2154
Size: 1334 820
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1118 1574 1864 2154
Size: 1118 456 290 290
Fragments arranged by size:
1118 456 290 290
AlwNI CAG_nnn'CTG
Cuts at: 0 1142 1465 1494 2154
Size: 1142 323 29 660
Fragments arranged by size:
1142 660 323 29
ApaI G_GGCC'C
Cuts at: 0 169 503 2154
Size: 169 334 1651
Fragments arranged by size:
1651 334 169
AvaI C'yCGr_G
Cuts at: 0 132 586 2154
Size: 132 454 1568
Fragments arranged by size:
1568 454 132
BanI G'GyrC_C
Cuts at: 0 136 174 238 265 2154
Size: 136 38 64 27 1889
Fragments arranged by size:
1889 136 64 38 27
BanII G_rGCy'C
Cuts at: 0 169 296 503 745 829 2145 2154
Size: 169 127 207 242 84 1316 9
Fragments arranged by size:
1316 242 207 169 127 84 9
BbeI G_GCGC'C
Cuts at: 0 140 178 242 269 2154
Size: 140 38 64 27 1885
Fragments arranged by size:
1885 140 64 38 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 2122 2154
Size: 2122 32
BclI T'GATC_A
Cuts at: 0 1035 2154
Size: 1035 1119
BglI GCCn_nnn'nGGC
Cuts at: 0 1147 2154
Size: 1147 1007
Bme1580I G_kGCm'C
Cuts at: 0 169 503 1163 1374 1512 2154
Size: 169 334 660 211 138 642
Fragments arranged by size:
660 642 334 211 169 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 945 1901 2154
Size: 945 956 253
Fragments arranged by size:
956 945 253
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1188 2154
Size: 1188 966
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1065 1693 2154
Size: 1065 628 461
Fragments arranged by size:
1065 628 461
Bpu10I CC'TnA_GC
Cuts at: 0 94 2154
Size: 94 2060
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1654 2154
Size: 1654 500
BsaBI GATnn'nnATC
Cuts at: 0 608 2154
Size: 608 1546
BsaHI Gr'CG_yC
Cuts at: 0 137 146 175 239 266 2154
Size: 137 9 29 64 27 1888
Fragments arranged by size:
1888 137 64 29 27 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 767 797 1796 1826 2154
Size: 238 30 499 30 999 30 328
Fragments arranged by size:
999 499 328 238 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 189 201 2154
Size: 189 12 1953
Fragments arranged by size:
1953 189 12
BseYI C'CCAG_C
Cuts at: 0 1132 1532 2154
Size: 1132 400 622
Fragments arranged by size:
1132 622 400
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1507 1694 2154
Size: 1507 187 460
Fragments arranged by size:
1507 460 187
BsiEI CG_ry'CG
Cuts at: 0 256 2154
Size: 256 1898
BsiHKAI G_wGCw'C
Cuts at: 0 745 1433 2145 2154
Size: 745 688 712 9
Fragments arranged by size:
745 712 688 9
BsmI GAATG_Cn'
Cuts at: 0 549 682 1407 1932 2154
Size: 549 133 725 525 222
Fragments arranged by size:
725 549 525 222 133
Bsp1286I G_dGCh'C
Cuts at: 0 169 296 503 745 829 1163 1374 1433
Size: 169 127 207 242 84 334 211 59
Cuts at: 1433 1512 2145 2154
Size: 79 633 9
Fragments arranged by size:
633 334 242 211 207 169 127 84
79 59 9
BspHI T'CATG_A
Cuts at: 0 74 2154
Size: 74 2080
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 531 771 1251 2154
Size: 531 240 480 903
Fragments arranged by size:
903 531 480 240
BsrBI CCG'CTC
Cuts at: 0 87 2154
Size: 87 2067
BssSI C'ACGA_G
Cuts at: 0 1162 2154
Size: 1162 992
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1465 2154
Size: 1465 689
BstYI r'GATC_y
Cuts at: 0 1491 1629 2102 2154
Size: 1491 138 473 52
Fragments arranged by size:
1491 473 138 52
BtgI C'CryG_G
Cuts at: 0 30 242 725 771 1151 2154
Size: 30 212 483 46 380 1003
Fragments arranged by size:
1003 483 380 212 46 30
BtsI GCAGTG_nn'
Cuts at: 0 642 763 1192 1466 1613 1949 2154
Size: 642 121 429 274 147 336 205
Fragments arranged by size:
642 429 336 274 205 147 121
DraIII CAC_nnn'GTG
Cuts at: 0 436 2154
Size: 436 1718
EaeI y'GGCC_r
Cuts at: 0 405 1836 2154
Size: 405 1431 318
Fragments arranged by size:
1431 405 318
EarI CTCTTCn'nnn_
Cuts at: 0 1185 2154
Size: 1185 969
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 50 1996 2154
Size: 50 1946 158
Fragments arranged by size:
1946 158 50
EcoICRI GAG'CTC
Cuts at: 0 743 2143 2154
Size: 743 1400 11
Fragments arranged by size:
1400 743 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1065 1104 1570 1693 1757 1777 1883
Size: 634 431 39 466 123 64 20 106
Cuts at: 1883 2137 2154
Size: 254 17
Fragments arranged by size:
634 466 431 254 123 106 64 39
20 17
EcoO109I rG'GnC_Cy
Cuts at: 0 166 325 499 2154
Size: 166 159 174 1655
Fragments arranged by size:
1655 174 166 159
HaeII r_GCGC'y
Cuts at: 0 140 178 242 269 282 1142 2154
Size: 140 38 64 27 13 860 1012
Fragments arranged by size:
1012 860 140 64 38 27 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 196 228 238 270 496 528 555 587
Size: 196 32 10 32 226 32 27 32
Cuts at: 587 767 799 2087 2119 2154
Size: 180 32 1288 32 35
Fragments arranged by size:
1288 226 196 180 35 32 32 32
32 32 32 27 10
HincII GTy'rAC
Cuts at: 0 1988 2154
Size: 1988 166
HindIII A'AGCT_T
Cuts at: 0 1014 2154
Size: 1014 1140
KasI G'GCGC_C
Cuts at: 0 136 174 238 265 2154
Size: 136 38 64 27 1889
Fragments arranged by size:
1889 136 64 38 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 218 227 2130 2154
Size: 218 9 1903 24
Fragments arranged by size:
1903 218 24 9
MscI TGG'CCA
Cuts at: 0 407 2154
Size: 407 1747
MslI CAynn'nnrTG
Cuts at: 0 614 1556 2154
Size: 614 942 598
Fragments arranged by size:
942 614 598
MspA1I CmG'CkG
Cuts at: 0 32 152 1810 1861 2154
Size: 32 120 1658 51 293
Fragments arranged by size:
1658 293 120 51 32
NarI GG'CG_CC
Cuts at: 0 137 175 239 266 2154
Size: 137 38 64 27 1888
Fragments arranged by size:
1888 137 64 38 27
NcoI C'CATG_G
Cuts at: 0 771 1151 2154
Size: 771 380 1003
Fragments arranged by size:
1003 771 380
NdeI CA'TA_TG
Cuts at: 0 299 2154
Size: 299 1855
NspI r_CATG'y
Cuts at: 0 886 1416 1555 2154
Size: 886 530 139 599
Fragments arranged by size:
886 599 530 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 414 2154
Size: 414 1740
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 959 991 2154
Size: 959 32 1163
Fragments arranged by size:
1163 959 32
PspOMI G'GGCC_C
Cuts at: 0 165 499 2154
Size: 165 334 1655
Fragments arranged by size:
1655 334 165
PvuII CAG'CTG
Cuts at: 0 152 1861 2154
Size: 152 1709 293
Fragments arranged by size:
1709 293 152
SacI G_AGCT'C
Cuts at: 0 745 2145 2154
Size: 745 1400 9
Fragments arranged by size:
1400 745 9
SacII CC_GC'GG
Cuts at: 0 33 2154
Size: 33 2121
ScaI AGT'ACT
Cuts at: 0 2040 2154
Size: 2040 114
SfoI GGC'GCC
Cuts at: 0 138 176 240 267 2154
Size: 138 38 64 27 1887
Fragments arranged by size:
1887 138 64 38 27
SmaI CCC'GGG
Cuts at: 0 134 588 2154
Size: 134 454 1566
Fragments arranged by size:
1566 454 134
SmlI C'TyrA_G
Cuts at: 0 1633 2154
Size: 1633 521
SspI AAT'ATT
Cuts at: 0 1056 1293 2154
Size: 1056 237 861
Fragments arranged by size:
1056 861 237
StyI C'CwwG_G
Cuts at: 0 771 1151 1359 2154
Size: 771 380 208 795
Fragments arranged by size:
795 771 380 208
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 147 922 2154
Size: 147 775 1232
Fragments arranged by size:
1232 775 147
TatI w'GTAC_w
Cuts at: 0 2038 2154
Size: 2038 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1796 1828 2154
Size: 1796 32 326
Fragments arranged by size:
1796 326 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 304 2154
Size: 304 1850
XmaI C'CCGG_G
Cuts at: 0 132 586 2154
Size: 132 454 1568
Fragments arranged by size:
1568 454 132
XmnI GAAnn'nnTTC
Cuts at: 0 605 1883 2154
Size: 605 1278 271
Fragments arranged by size:
1278 605 271
Enzymes that do cut:
AarI AcuI AflIII AlfI AlwNI ApaI AvaI BanI
BanII BbeI BciVI BclI BglI Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BssSI BstAPI BstYI BtgI BtsI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII HindIII
KasI MmeI MscI MslI MspA1I NarI NcoI NdeI
NspI PflMI PpiI PspOMI PvuII SacI SacII ScaI
SfoI SmaI SmlI SspI StyI TaqII TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbsI BbvCI BcgI BfrBI BglII BlpI
BmgBI BmtI BsaI BsaAI BsaWI BsiWI BsmBI BspEI
BsrDI BsrFI BsrGI BssHII BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DrdI EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HpaI KpnI
MfeI MluI NaeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PfoI PmeI PmlI PpuMI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI
SapI SbfI SexAI SfcI SfiI SgfI SgrAI SnaBI
SpeI SphI SrfI StuI SwaI Tth111I XbaI XhoI
ZraI