Restriction map for
pF1KB5707
(Linear) (Six-base) MAPSORT of: KIBB5707.seq Check: 9303 from: 1 to: 2346
KIBB5707.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1026 1874 2346
Size: 1026 848 472
Fragments arranged by size:
1026 848 472
AatII G_ACGT'C
Cuts at: 0 426 511 2346
Size: 426 85 1835
Fragments arranged by size:
1835 426 85
AccI GT'mk_AC
Cuts at: 0 511 1378 2346
Size: 511 867 968
Fragments arranged by size:
968 867 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 379 662 1632 2004 2279 2346
Size: 379 283 970 372 275 67
Fragments arranged by size:
970 379 372 283 275 67
AflII C'TTAA_G
Cuts at: 0 286 2346
Size: 286 2060
AflIII A'CryG_T
Cuts at: 0 194 2346
Size: 194 2152
AlwNI CAG_nnn'CTG
Cuts at: 0 1845 2346
Size: 1845 501
ApoI r'AATT_y
Cuts at: 0 583 874 1595 2346
Size: 583 291 721 751
Fragments arranged by size:
751 721 583 291
BanII G_rGCy'C
Cuts at: 0 822 949 1373 1901 2346
Size: 822 127 424 528 445
Fragments arranged by size:
822 528 445 424 127
BbsI GAAGACnn'nnnn_
Cuts at: 0 1292 2346
Size: 1292 1054
BbvCI CC'TCA_GC
Cuts at: 0 265 541 1340 2346
Size: 265 276 799 1006
Fragments arranged by size:
1006 799 276 265
BciVI GTATCCnnnnn_n'
Cuts at: 0 242 2346
Size: 242 2104
BclI T'GATC_A
Cuts at: 0 29 2346
Size: 29 2317
BglII A'GATC_T
Cuts at: 0 1059 2346
Size: 1059 1287
BlpI GC'TnA_GC
Cuts at: 0 327 2346
Size: 327 2019
BmgBI CAC'GTC
Cuts at: 0 195 2205 2346
Size: 195 2010 141
Fragments arranged by size:
2010 195 141
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 124 1355 1898 2346
Size: 124 1231 543 448
Fragments arranged by size:
1231 543 448 124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 1366 2346
Size: 100 1266 980
Fragments arranged by size:
1266 980 100
Bpu10I CC'TnA_GC
Cuts at: 0 265 541 578 1340 2346
Size: 265 276 37 762 1006
Fragments arranged by size:
1006 762 276 265 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 963 1399 2346
Size: 963 436 947
Fragments arranged by size:
963 947 436
BsaI GGTCTCn'nnnn_
Cuts at: 0 791 2346
Size: 791 1555
BsaAI yAC'GTr
Cuts at: 0 1625 2346
Size: 1625 721
BsaHI Gr'CG_yC
Cuts at: 0 423 508 1891 2346
Size: 423 85 1383 455
Fragments arranged by size:
1383 455 423 85
BsaWI w'CCGG_w
Cuts at: 0 1455 2346
Size: 1455 891
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 202 232 922 952 1826 1856 2346
Size: 202 30 690 30 874 30 490
Fragments arranged by size:
874 690 490 202 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 939 1264 2346
Size: 223 716 325 1082
Fragments arranged by size:
1082 716 325 223
BseYI C'CCAG_C
Cuts at: 0 1165 2346
Size: 1165 1181
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1852 2346
Size: 1852 494
BsiHKAI G_wGCw'C
Cuts at: 0 526 579 856 949 2346
Size: 526 53 277 93 1397
Fragments arranged by size:
1397 526 277 93 53
BsmI GAATG_Cn'
Cuts at: 0 2323 2346
Size: 2323 23
BsmBI CGTCTCn'nnnn_
Cuts at: 0 97 185 2211 2346
Size: 97 88 2026 135
Fragments arranged by size:
2026 135 97 88
Bsp1286I G_dGCh'C
Cuts at: 0 526 579 822 856 949 1373 1901 2346
Size: 526 53 243 34 93 424 528 445
Fragments arranged by size:
528 526 445 424 243 93 53 34
BspEI T'CCGG_A
Cuts at: 0 1455 2346
Size: 1455 891
BspHI T'CATG_A
Cuts at: 0 1098 2153 2346
Size: 1098 1055 193
Fragments arranged by size:
1098 1055 193
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 523 1026 1874 2185 2346
Size: 523 503 848 311 161
Fragments arranged by size:
848 523 503 311 161
BsrDI GCAATG_nn'
Cuts at: 0 398 2051 2346
Size: 398 1653 295
Fragments arranged by size:
1653 398 295
BsrGI T'GTAC_A
Cuts at: 0 395 2346
Size: 395 1951
BssSI C'ACGA_G
Cuts at: 0 54 2346
Size: 54 2292
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2143 2346
Size: 2143 203
BstXI CCAn_nnnn'nTGG
Cuts at: 0 602 2245 2346
Size: 602 1643 101
Fragments arranged by size:
1643 602 101
BstYI r'GATC_y
Cuts at: 0 702 1059 1787 2346
Size: 702 357 728 559
Fragments arranged by size:
728 702 559 357
BstZ17I GTA'TAC
Cuts at: 0 1379 2346
Size: 1379 967
BtgI C'CryG_G
Cuts at: 0 527 764 2346
Size: 527 237 1582
Fragments arranged by size:
1582 527 237
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1136 2346
Size: 1136 1210
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1073 1108 2346
Size: 1073 35 1238
Fragments arranged by size:
1238 1073 35
EaeI y'GGCC_r
Cuts at: 0 1930 2346
Size: 1930 416
EarI CTCTTCn'nnn_
Cuts at: 0 775 810 2127 2346
Size: 775 35 1317 219
Fragments arranged by size:
1317 775 219 35
EcoICRI GAG'CTC
Cuts at: 0 947 2346
Size: 947 1399
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 379 662 1366 1632 2004 2279 2346
Size: 100 279 283 704 266 372 275 67
Fragments arranged by size:
704 372 283 279 275 266 100 67
EcoO109I rG'GnC_Cy
Cuts at: 0 1216 2346
Size: 1216 1130
EcoRV GAT'ATC
Cuts at: 0 453 2346
Size: 453 1893
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 202 234 640 672 769 801
Size: 121 32 49 32 406 32 97 32
Cuts at: 801 922 954 2148 2180 2346
Size: 121 32 1194 32 166
Fragments arranged by size:
1194 406 166 121 121 97 49 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1566 2346
Size: 1566 780
HindIII A'AGCT_T
Cuts at: 0 1427 2093 2346
Size: 1427 666 253
Fragments arranged by size:
1427 666 253
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 2346
Size: 724 1622
MspA1I CmG'CkG
Cuts at: 0 216 264 835 966 1344 1612 2346
Size: 216 48 571 131 378 268 734
Fragments arranged by size:
734 571 378 268 216 131 48
NcoI C'CATG_G
Cuts at: 0 764 2346
Size: 764 1582
PasI CC'CwG_GG
Cuts at: 0 1138 2346
Size: 1138 1208
PflMI CCAn_nnn'nTGG
Cuts at: 0 1192 2346
Size: 1192 1154
PfoI T'CCnGG_A
Cuts at: 0 1684 2346
Size: 1684 662
PsiI TTA'TAA
Cuts at: 0 2039 2346
Size: 2039 307
PstI C_TGCA'G
Cuts at: 0 1841 2009 2346
Size: 1841 168 337
Fragments arranged by size:
1841 337 168
PvuII CAG'CTG
Cuts at: 0 216 264 1344 1612 2346
Size: 216 48 1080 268 734
Fragments arranged by size:
1080 734 268 216 48
SacI G_AGCT'C
Cuts at: 0 949 2346
Size: 949 1397
SapI GCTCTTCn'nnn_
Cuts at: 0 810 2127 2346
Size: 810 1317 219
Fragments arranged by size:
1317 810 219
SexAI A'CCwGG_T
Cuts at: 0 514 2346
Size: 514 1832
SfcI C'TryA_G
Cuts at: 0 1837 2005 2346
Size: 1837 168 341
Fragments arranged by size:
1837 341 168
SmlI C'TyrA_G
Cuts at: 0 286 942 1414 2346
Size: 286 656 472 932
Fragments arranged by size:
932 656 472 286
SspI AAT'ATT
Cuts at: 0 2290 2346
Size: 2290 56
StyI C'CwwG_G
Cuts at: 0 764 2346
Size: 764 1582
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1676 1845 2346
Size: 1676 169 501
Fragments arranged by size:
1676 501 169
TatI w'GTAC_w
Cuts at: 0 395 560 2305 2346
Size: 395 165 1745 41
Fragments arranged by size:
1745 395 165 41
ZraI GAC'GTC
Cuts at: 0 424 509 2346
Size: 424 85 1837
Fragments arranged by size:
1837 424 85
Enzymes that do cut:
AarI AatII AccI AcuI AflII AflIII AlwNI ApoI
BanII BbsI BbvCI BciVI BclI BglII BlpI BmgBI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI
BstYI BstZ17I BtgI BtgZI CspCI EaeI EarI EcoICRI
Eco57MI EcoO109I EcoRV Hin4I HincII HindIII MmeI MspA1I
NcoI PasI PflMI PfoI PsiI PstI PvuII SacI
SapI SexAI SfcI SmlI SspI StyI TaqII TatI
ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AgeI AhdI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BcgI BfrBI BglI Bme1580I BmrI
BmtI BsaBI BsiEI BsiWI BsrBI BsrFI BssHII BstBI
BstEII Bsu36I BtsI ClaI DraI DraIII DrdI EagI
EciI EcoNI EcoRI FalI FseI FspI FspAI HaeII
HpaI KasI KpnI MfeI MluI MscI MslI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI