Restriction map for
pF1KB5674
(Linear) (Six-base) MAPSORT of: KIBB5674.seq Check: 248 from: 1 to: 1305
KIBB5674.orf
With 182 enzymes: SgfI *
February 28, 2007 11:28 ..
AatII G_ACGT'C
Cuts at: 0 146 1305
Size: 146 1159
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 1124 1305
Size: 458 666 181
Fragments arranged by size:
666 458 181
AflIII A'CryG_T
Cuts at: 0 1235 1305
Size: 1235 70
AleI CACnn'nnGTG
Cuts at: 0 644 1305
Size: 644 661
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 662 1305
Size: 662 643
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 1305
Size: 1 32 1272
Fragments arranged by size:
1272 32 1
ApaI G_GGCC'C
Cuts at: 0 259 629 1305
Size: 259 370 676
Fragments arranged by size:
676 370 259
ApaLI G'TGCA_C
Cuts at: 0 637 1305
Size: 637 668
ApoI r'AATT_y
Cuts at: 0 414 835 1305
Size: 414 421 470
Fragments arranged by size:
470 421 414
AvaI C'yCGr_G
Cuts at: 0 503 907 1183 1305
Size: 503 404 276 122
Fragments arranged by size:
503 404 276 122
BanI G'GyrC_C
Cuts at: 0 829 1141 1305
Size: 829 312 164
Fragments arranged by size:
829 312 164
BanII G_rGCy'C
Cuts at: 0 259 629 902 1305
Size: 259 370 273 403
Fragments arranged by size:
403 370 273 259
BbsI GAAGACnn'nnnn_
Cuts at: 0 413 1126 1305
Size: 413 713 179
Fragments arranged by size:
713 413 179
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 300 334 1183 1217 1305
Size: 300 34 849 34 88
Fragments arranged by size:
849 300 88 34 34
BglII A'GATC_T
Cuts at: 0 509 1305
Size: 509 796
Bme1580I G_kGCm'C
Cuts at: 0 259 629 641 1146 1305
Size: 259 370 12 505 159
Fragments arranged by size:
505 370 259 159 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 970 1305
Size: 916 54 335
Fragments arranged by size:
916 335 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 1003 1305
Size: 1003 302
BsaHI Gr'CG_yC
Cuts at: 0 143 1305
Size: 143 1162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 1305
Size: 280 30 995
Fragments arranged by size:
995 280 30
BseYI C'CCAG_C
Cuts at: 0 44 1158 1305
Size: 44 1114 147
Fragments arranged by size:
1114 147 44
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 668 880 1305
Size: 668 212 425
Fragments arranged by size:
668 425 212
BsiEI CG_ry'CG
Cuts at: 0 1262 1305
Size: 1262 43
BsiHKAI G_wGCw'C
Cuts at: 0 623 641 1305
Size: 623 18 664
Fragments arranged by size:
664 623 18
BsmBI CGTCTCn'nnnn_
Cuts at: 0 134 1305
Size: 134 1171
Bsp1286I G_dGCh'C
Cuts at: 0 259 623 629 641 902 1146 1305
Size: 259 364 6 12 261 244 159
Fragments arranged by size:
364 261 259 244 159 12 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 650 697 1305
Size: 650 47 608
Fragments arranged by size:
650 608 47
BsrFI r'CCGG_y
Cuts at: 0 1261 1305
Size: 1261 44
BssSI C'ACGA_G
Cuts at: 0 886 1305
Size: 886 419
BstYI r'GATC_y
Cuts at: 0 509 1305
Size: 509 796
BtsI GCAGTG_nn'
Cuts at: 0 639 651 1177 1305
Size: 639 12 526 128
Fragments arranged by size:
639 526 128 12
DrdI GACnn_nn'nnGTC
Cuts at: 0 547 1305
Size: 547 758
EaeI y'GGCC_r
Cuts at: 0 50 101 373 931 1259 1305
Size: 50 51 272 558 328 46
Fragments arranged by size:
558 328 272 51 50 46
EagI C'GGCC_G
Cuts at: 0 1259 1305
Size: 1259 46
EarI CTCTTCn'nnn_
Cuts at: 0 858 1001 1305
Size: 858 143 304
Fragments arranged by size:
858 304 143
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 458 916 970 1124 1305
Size: 458 458 54 154 181
Fragments arranged by size:
458 458 181 154 54
EcoO109I rG'GnC_Cy
Cuts at: 0 255 256 891 1305
Size: 255 1 635 414
Fragments arranged by size:
635 414 255 1
EcoRV GAT'ATC
Cuts at: 0 66 444 735 1305
Size: 66 378 291 570
Fragments arranged by size:
570 378 291 66
FspI TGC'GCA
Cuts at: 0 363 1305
Size: 363 942
FspAI rTGC'GCAy
Cuts at: 0 363 1305
Size: 363 942
HaeII r_GCGC'y
Cuts at: 0 1268 1305
Size: 1268 37
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 433 465 589 621 1305
Size: 433 32 124 32 684
Fragments arranged by size:
684 433 124 32 32
HincII GTy'rAC
Cuts at: 0 1233 1305
Size: 1233 72
HindIII A'AGCT_T
Cuts at: 0 106 1305
Size: 106 1199
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 93 145 1305
Size: 93 52 1160
Fragments arranged by size:
1160 93 52
MscI TGG'CCA
Cuts at: 0 103 375 1305
Size: 103 272 930
Fragments arranged by size:
930 272 103
MslI CAynn'nnrTG
Cuts at: 0 644 1240 1305
Size: 644 596 65
Fragments arranged by size:
644 596 65
MspA1I CmG'CkG
Cuts at: 0 798 1305
Size: 798 507
NaeI GCC'GGC
Cuts at: 0 1263 1305
Size: 1263 42
NgoMIV G'CCGG_C
Cuts at: 0 1261 1305
Size: 1261 44
NspI r_CATG'y
Cuts at: 0 275 1239 1305
Size: 275 964 66
Fragments arranged by size:
964 275 66
PasI CC'CwG_GG
Cuts at: 0 252 1305
Size: 252 1053
PciI A'CATG_T
Cuts at: 0 1235 1305
Size: 1235 70
PflMI CCAn_nnn'nTGG
Cuts at: 0 961 1305
Size: 961 344
PfoI T'CCnGG_A
Cuts at: 0 37 947 1305
Size: 37 910 358
Fragments arranged by size:
910 358 37
PpuMI rG'GwC_Cy
Cuts at: 0 891 1305
Size: 891 414
PshAI GACnn'nnGTC
Cuts at: 0 146 161 1305
Size: 146 15 1144
Fragments arranged by size:
1144 146 15
PspOMI G'GGCC_C
Cuts at: 0 255 625 1305
Size: 255 370 680
Fragments arranged by size:
680 370 255
PstI C_TGCA'G
Cuts at: 0 646 1305
Size: 646 659
PvuII CAG'CTG
Cuts at: 0 798 1305
Size: 798 507
SfcI C'TryA_G
Cuts at: 0 642 1278 1305
Size: 642 636 27
Fragments arranged by size:
642 636 27
SmlI C'TyrA_G
Cuts at: 0 503 1305
Size: 503 802
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 951 987 1305
Size: 951 36 318
Fragments arranged by size:
951 318 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 280 312 1305
Size: 280 32 993
Fragments arranged by size:
993 280 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 840 945 992 1305
Size: 840 105 47 313
Fragments arranged by size:
840 313 105 47
XhoI C'TCGA_G
Cuts at: 0 503 1305
Size: 503 802
XmnI GAAnn'nnTTC
Cuts at: 0 1222 1305
Size: 1222 83
ZraI GAC'GTC
Cuts at: 0 144 1305
Size: 144 1161
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AloI ApaI ApaLI
ApoI AvaI BanI BanII BbsI BcgI BglII Bme1580I
BpmI BsaI BsaHI BsaXI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrFI BssSI BstYI BtsI DrdI
EaeI EagI EarI Eco57MI EcoO109I EcoRV FspI FspAI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI
PshAI PspOMI PstI PvuII SfcI SmlI TaqII TstI
XcmI XhoI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AlwNI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BclI BfrBI BglI BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaWI BseRI
BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII EciI EcoICRI EcoNI EcoRI
FalI FseI HpaI KasI KpnI MfeI MluI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI TatI Tth111I XbaI XmaI