Restriction map for
pF1KB5670
(Linear) (Six-base) MAPSORT of: KIBB5670.seq Check: 9846 from: 1 to: 2775
KIBB5670.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 439 2775
Size: 439 2336
AccI GT'mk_AC
Cuts at: 0 2532 2775
Size: 2532 243
AclI AA'CG_TT
Cuts at: 0 324 2775
Size: 324 2451
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1178 1695 2287 2775
Size: 671 507 517 592 488
Fragments arranged by size:
671 592 517 507 488
AflIII A'CryG_T
Cuts at: 0 2261 2775
Size: 2261 514
AleI CACnn'nnGTG
Cuts at: 0 2034 2775
Size: 2034 741
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 2775
Size: 190 32 2553
Fragments arranged by size:
2553 190 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2098 2709 2775
Size: 2098 611 66
Fragments arranged by size:
2098 611 66
ApaI G_GGCC'C
Cuts at: 0 148 2775
Size: 148 2627
ApoI r'AATT_y
Cuts at: 0 337 413 672 850 1408 1493 1507 2362
Size: 337 76 259 178 558 85 14 855
Cuts at: 2362 2775
Size: 413
Fragments arranged by size:
855 558 413 337 259 178 85 76
14
AseI AT'TA_AT
Cuts at: 0 395 2775
Size: 395 2380
BamHI G'GATC_C
Cuts at: 0 1806 2775
Size: 1806 969
BanII G_rGCy'C
Cuts at: 0 148 191 2775
Size: 148 43 2584
Fragments arranged by size:
2584 148 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 58 1841 2775
Size: 58 1783 934
Fragments arranged by size:
1783 934 58
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1458 1492 2585 2619 2775
Size: 1458 34 1093 34 156
Fragments arranged by size:
1458 1093 156 34 34
BclI T'GATC_A
Cuts at: 0 1154 2775
Size: 1154 1621
BfrBI ATG'CAT
Cuts at: 0 1559 2050 2775
Size: 1559 491 725
Fragments arranged by size:
1559 725 491
BglII A'GATC_T
Cuts at: 0 594 1752 2775
Size: 594 1158 1023
Fragments arranged by size:
1158 1023 594
BlpI GC'TnA_GC
Cuts at: 0 1427 2775
Size: 1427 1348
Bme1580I G_kGCm'C
Cuts at: 0 148 2595 2775
Size: 148 2447 180
Fragments arranged by size:
2447 180 148
BmgBI CAC'GTC
Cuts at: 0 1990 2775
Size: 1990 785
BmrI ACTGGGnnnn_n'
Cuts at: 0 895 991 2625 2775
Size: 895 96 1634 150
Fragments arranged by size:
1634 895 150 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 995 1357 1366 2717 2775
Size: 175 820 362 9 1351 58
Fragments arranged by size:
1351 820 362 175 58 9
Bpu10I CC'TnA_GC
Cuts at: 0 76 2775
Size: 76 2699
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 619 2775
Size: 157 462 2156
Fragments arranged by size:
2156 462 157
BsaWI w'CCGG_w
Cuts at: 0 47 2775
Size: 47 2728
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 2775
Size: 316 2459
BseYI C'CCAG_C
Cuts at: 0 1810 2775
Size: 1810 965
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 133 473 2775
Size: 133 340 2302
Fragments arranged by size:
2302 340 133
BsiHKAI G_wGCw'C
Cuts at: 0 191 792 1914 2775
Size: 191 601 1122 861
Fragments arranged by size:
1122 861 601 191
BsmI GAATG_Cn'
Cuts at: 0 475 658 1123 2050 2488 2524 2775
Size: 475 183 465 927 438 36 251
Fragments arranged by size:
927 475 465 438 251 183 36
Bsp1286I G_dGCh'C
Cuts at: 0 148 191 792 1914 2595 2775
Size: 148 43 601 1122 681 180
Fragments arranged by size:
1122 681 601 180 148 43
BspEI T'CCGG_A
Cuts at: 0 47 2775
Size: 47 2728
BspHI T'CATG_A
Cuts at: 0 2355 2775
Size: 2355 420
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 229 439 2775
Size: 229 210 2336
Fragments arranged by size:
2336 229 210
BsrDI GCAATG_nn'
Cuts at: 0 1722 1821 2114 2163 2775
Size: 1722 99 293 49 612
Fragments arranged by size:
1722 612 293 99 49
BsrGI T'GTAC_A
Cuts at: 0 710 2264 2775
Size: 710 1554 511
Fragments arranged by size:
1554 710 511
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2435 2775
Size: 2435 340
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1216 2775
Size: 1216 1559
BstYI r'GATC_y
Cuts at: 0 594 645 896 1189 1752 1806 2775
Size: 594 51 251 293 563 54 969
Fragments arranged by size:
969 594 563 293 251 54 51
BtgI C'CryG_G
Cuts at: 0 210 2775
Size: 210 2565
BtsI GCAGTG_nn'
Cuts at: 0 820 1201 2775
Size: 820 381 1574
Fragments arranged by size:
1574 820 381
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2043 2076 2078 2111 2775
Size: 2043 33 2 33 664
Fragments arranged by size:
2043 664 33 33 2
DraI TTT'AAA
Cuts at: 0 320 342 723 1413 1928 2775
Size: 320 22 381 690 515 847
Fragments arranged by size:
847 690 515 381 320 22
EarI CTCTTCn'nnn_
Cuts at: 0 101 1583 1853 2187 2775
Size: 101 1482 270 334 588
Fragments arranged by size:
1482 588 334 270 101
EcoICRI GAG'CTC
Cuts at: 0 189 2775
Size: 189 2586
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 671 995 1178 1357 1366 1695 2287
Size: 175 496 324 183 179 9 329 592
Cuts at: 2287 2717 2775
Size: 430 58
Fragments arranged by size:
592 496 430 329 324 183 179 175
58 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 96 1066 2775
Size: 96 970 1709
Fragments arranged by size:
1709 970 96
EcoO109I rG'GnC_Cy
Cuts at: 0 144 2775
Size: 144 2631
EcoRI G'AATT_C
Cuts at: 0 672 2775
Size: 672 2103
EcoRV GAT'ATC
Cuts at: 0 366 2775
Size: 366 2409
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2538 2775
Size: 2538 237
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 278 2775
Size: 164 114 2497
Fragments arranged by size:
2497 164 114
MslI CAynn'nnrTG
Cuts at: 0 2014 2034 2167 2775
Size: 2014 20 133 608
Fragments arranged by size:
2014 608 133 20
MspA1I CmG'CkG
Cuts at: 0 985 1431 2461 2775
Size: 985 446 1030 314
Fragments arranged by size:
1030 985 446 314
NsiI A_TGCA'T
Cuts at: 0 1561 2052 2775
Size: 1561 491 723
Fragments arranged by size:
1561 723 491
NspI r_CATG'y
Cuts at: 0 1559 1672 2265 2453 2775
Size: 1559 113 593 188 322
Fragments arranged by size:
1559 593 322 188 113
PacI TTA_AT'TAA
Cuts at: 0 395 2775
Size: 395 2380
PciI A'CATG_T
Cuts at: 0 2261 2775
Size: 2261 514
PfoI T'CCnGG_A
Cuts at: 0 35 191 2775
Size: 35 156 2584
Fragments arranged by size:
2584 156 35
PsiI TTA'TAA
Cuts at: 0 1361 2659 2775
Size: 1361 1298 116
Fragments arranged by size:
1361 1298 116
PspOMI G'GGCC_C
Cuts at: 0 144 2775
Size: 144 2631
PstI C_TGCA'G
Cuts at: 0 1784 2431 2608 2775
Size: 1784 647 177 167
Fragments arranged by size:
1784 647 177 167
PvuII CAG'CTG
Cuts at: 0 985 1431 2461 2775
Size: 985 446 1030 314
Fragments arranged by size:
1030 985 446 314
SacI G_AGCT'C
Cuts at: 0 191 2775
Size: 191 2584
SapI GCTCTTCn'nnn_
Cuts at: 0 2187 2775
Size: 2187 588
SfcI C'TryA_G
Cuts at: 0 418 794 1780 2427 2604 2775
Size: 418 376 986 647 177 171
Fragments arranged by size:
986 647 418 376 177 171
SmlI C'TyrA_G
Cuts at: 0 172 598 2775
Size: 172 426 2177
Fragments arranged by size:
2177 426 172
SpeI A'CTAG_T
Cuts at: 0 1129 2224 2775
Size: 1129 1095 551
Fragments arranged by size:
1129 1095 551
SphI G_CATG'C
Cuts at: 0 1559 1672 2775
Size: 1559 113 1103
Fragments arranged by size:
1559 1103 113
SspI AAT'ATT
Cuts at: 0 2076 2775
Size: 2076 699
StyI C'CwwG_G
Cuts at: 0 1268 2444 2775
Size: 1268 1176 331
Fragments arranged by size:
1268 1176 331
SwaI ATTT'AAAT
Cuts at: 0 1413 2775
Size: 1413 1362
TatI w'GTAC_w
Cuts at: 0 710 2264 2633 2711 2775
Size: 710 1554 369 78 64
Fragments arranged by size:
1554 710 369 78 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2612 2644 2775
Size: 2612 32 131
Fragments arranged by size:
2612 131 32
XbaI T'CTAG_A
Cuts at: 0 1170 2775
Size: 1170 1605
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1914 2775
Size: 1914 861
XmnI GAAnn'nnTTC
Cuts at: 0 413 473 541 1375 1705 2336 2775
Size: 413 60 68 834 330 631 439
Fragments arranged by size:
834 631 439 413 330 68 60
Enzymes that do cut:
AarI AccI AclI AcuI AflIII AleI AloI AlwNI
ApaI ApoI AseI BamHI BanII BbsI BcgI BclI
BfrBI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BpuEI BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrDI BsrGI BstAPI BstXI BstYI
BtgI BtsI CspCI DraI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI MmeI MslI MspA1I NsiI
NspI PacI PciI PfoI PsiI PspOMI PstI PvuII
SacI SapI SfcI SmlI SpeI SphI SspI StyI
SwaI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AgeI AhdI AlfI ApaLI
AscI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI
BciVI BglI BmtI BplI BsaI BsaAI BsaBI BsaHI
BsaXI BsiEI BsiWI BsmBI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraIII DrdI
EaeI EagI EciI FseI FspI FspAI HaeII Hin4I
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SrfI
StuI TaqII Tth111I XhoI XmaI ZraI