Restriction map for
pF1KB5664
(Linear) (Six-base) MAPSORT of: KIBB5664.seq Check: 2222 from: 1 to: 1134
KIBB5664.orf
With 182 enzymes: SgfI *
July 18, 2007 13:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 385 1134
Size: 385 749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 1096 1134
Size: 115 981 38
Fragments arranged by size:
981 115 38
AleI CACnn'nnGTG
Cuts at: 0 127 1134
Size: 127 1007
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 928 1134
Size: 928 206
ApaI G_GGCC'C
Cuts at: 0 969 1134
Size: 969 165
ApoI r'AATT_y
Cuts at: 0 205 1134
Size: 205 929
BanI G'GyrC_C
Cuts at: 0 452 505 1134
Size: 452 53 629
Fragments arranged by size:
629 452 53
BanII G_rGCy'C
Cuts at: 0 969 1134
Size: 969 165
BbeI G_GCGC'C
Cuts at: 0 509 1134
Size: 509 625
BbsI GAAGACnn'nnnn_
Cuts at: 0 310 453 1064 1134
Size: 310 143 611 70
Fragments arranged by size:
611 310 143 70
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 138 172 336 370 1134
Size: 138 34 164 34 764
Fragments arranged by size:
764 164 138 34 34
BclI T'GATC_A
Cuts at: 0 419 1134
Size: 419 715
BglI GCCn_nnn'nGGC
Cuts at: 0 367 1134
Size: 367 767
Bme1580I G_kGCm'C
Cuts at: 0 823 969 1134
Size: 823 146 165
Fragments arranged by size:
823 165 146
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 1134
Size: 10 1124
BsaI GGTCTCn'nnnn_
Cuts at: 0 40 1134
Size: 40 1094
BsaAI yAC'GTr
Cuts at: 0 72 1134
Size: 72 1062
BsaBI GATnn'nnATC
Cuts at: 0 187 1134
Size: 187 947
BsaHI Gr'CG_yC
Cuts at: 0 370 440 506 1134
Size: 370 70 66 628
Fragments arranged by size:
628 370 70 66
BseYI C'CCAG_C
Cuts at: 0 501 1134
Size: 501 633
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 832 1134
Size: 520 312 302
Fragments arranged by size:
520 312 302
BsiHKAI G_wGCw'C
Cuts at: 0 134 866 1134
Size: 134 732 268
Fragments arranged by size:
732 268 134
BsmBI CGTCTCn'nnnn_
Cuts at: 0 28 1134
Size: 28 1106
Bsp1286I G_dGCh'C
Cuts at: 0 134 823 866 969 1134
Size: 134 689 43 103 165
Fragments arranged by size:
689 165 134 103 43
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 385 1070 1134
Size: 263 122 685 64
Fragments arranged by size:
685 263 122 64
BsrBI CCG'CTC
Cuts at: 0 405 708 1134
Size: 405 303 426
Fragments arranged by size:
426 405 303
BsrDI GCAATG_nn'
Cuts at: 0 488 587 1134
Size: 488 99 547
Fragments arranged by size:
547 488 99
BsrFI r'CCGG_y
Cuts at: 0 30 262 1134
Size: 30 232 872
Fragments arranged by size:
872 232 30
BstEII G'GTnAC_C
Cuts at: 0 624 1134
Size: 624 510
BstYI r'GATC_y
Cuts at: 0 1121 1134
Size: 1121 13
Bsu36I CC'TnA_GG
Cuts at: 0 337 1134
Size: 337 797
BtgI C'CryG_G
Cuts at: 0 323 713 731 1134
Size: 323 390 18 403
Fragments arranged by size:
403 390 323 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 252 378 741 1134
Size: 252 126 363 393
Fragments arranged by size:
393 363 252 126
BtsI GCAGTG_nn'
Cuts at: 0 718 1134
Size: 718 416
EaeI y'GGCC_r
Cuts at: 0 260 389 614 686 899 1134
Size: 260 129 225 72 213 235
Fragments arranged by size:
260 235 225 213 129 72
EarI CTCTTCn'nnn_
Cuts at: 0 139 781 790 835 1134
Size: 139 642 9 45 299
Fragments arranged by size:
642 299 139 45 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 861 1134
Size: 861 273
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 115 1096 1134
Size: 10 105 981 38
Fragments arranged by size:
981 105 38 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 340 832 1134
Size: 340 492 302
Fragments arranged by size:
492 340 302
EcoO109I rG'GnC_Cy
Cuts at: 0 965 1134
Size: 965 169
HaeII r_GCGC'y
Cuts at: 0 509 1134
Size: 509 625
HincII GTy'rAC
Cuts at: 0 282 1134
Size: 282 852
KasI G'GCGC_C
Cuts at: 0 505 1134
Size: 505 629
MscI TGG'CCA
Cuts at: 0 391 688 901 1134
Size: 391 297 213 233
Fragments arranged by size:
391 297 233 213
MslI CAynn'nnrTG
Cuts at: 0 127 289 622 796 1134
Size: 127 162 333 174 338
Fragments arranged by size:
338 333 174 162 127
NaeI GCC'GGC
Cuts at: 0 264 1134
Size: 264 870
NarI GG'CG_CC
Cuts at: 0 506 1134
Size: 506 628
NcoI C'CATG_G
Cuts at: 0 713 1134
Size: 713 421
NgoMIV G'CCGG_C
Cuts at: 0 262 1134
Size: 262 872
NspI r_CATG'y
Cuts at: 0 222 1134
Size: 222 912
PflMI CCAn_nnn'nTGG
Cuts at: 0 72 329 1134
Size: 72 257 805
Fragments arranged by size:
805 257 72
PspOMI G'GGCC_C
Cuts at: 0 965 1134
Size: 965 169
PstI C_TGCA'G
Cuts at: 0 151 382 808 1134
Size: 151 231 426 326
Fragments arranged by size:
426 326 231 151
RsrII CG'GwC_CG
Cuts at: 0 728 1134
Size: 728 406
SapI GCTCTTCn'nnn_
Cuts at: 0 139 1134
Size: 139 995
SbfI CC_TGCA'GG
Cuts at: 0 808 1134
Size: 808 326
SfcI C'TryA_G
Cuts at: 0 147 378 804 1134
Size: 147 231 426 330
Fragments arranged by size:
426 330 231 147
SfoI GGC'GCC
Cuts at: 0 507 1134
Size: 507 627
StuI AGG'CCT
Cuts at: 0 103 432 1134
Size: 103 329 702
Fragments arranged by size:
702 329 103
StyI C'CwwG_G
Cuts at: 0 363 713 1134
Size: 363 350 421
Fragments arranged by size:
421 363 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 993 1134
Size: 993 141
TatI w'GTAC_w
Cuts at: 0 221 584 1134
Size: 221 363 550
Fragments arranged by size:
550 363 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 764 796 1134
Size: 764 32 338
Fragments arranged by size:
764 338 32
XmnI GAAnn'nnTTC
Cuts at: 0 307 1134
Size: 307 827
Enzymes that do cut:
AarI AcuI AleI AlfI ApaI ApoI BanI BanII
BbeI BbsI BcgI BclI BglI Bme1580I BpmI BsaI
BsaAI BsaBI BsaHI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BstEII BstYI Bsu36I BtgI
BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I
HaeII HincII KasI MscI MslI NaeI NarI NcoI
NgoMIV NspI PflMI PspOMI PstI RsrII SapI SbfI
SfcI SfoI StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbvCI BciVI BfrBI BglII BlpI
BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaWI BsaXI
BseRI BsiEI BsiWI BsmI BspEI BspHI BsrGI BssHII
BssSI BstAPI BstBI BstXI BstZ17I ClaI CspCI DraI
DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KpnI MfeI MluI
MmeI MspA1I NdeI NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI PvuII SacI SacII SalI
SanDI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI
XcmI XhoI XmaI ZraI