Restriction map for
pF1KB5651
(Linear) (Six-base) MAPSORT of: KIBB5651.seq Check: 4281 from: 1 to: 1200
KIBB5651.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 786 1200
Size: 786 414
AclI AA'CG_TT
Cuts at: 0 1103 1200
Size: 1103 97
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1148 1200
Size: 694 454 52
Fragments arranged by size:
694 454 52
AleI CACnn'nnGTG
Cuts at: 0 526 1200
Size: 526 674
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 791 1200
Size: 791 409
AlwNI CAG_nnn'CTG
Cuts at: 0 486 702 1200
Size: 486 216 498
Fragments arranged by size:
498 486 216
AvaI C'yCGr_G
Cuts at: 0 219 381 1200
Size: 219 162 819
Fragments arranged by size:
819 219 162
AvrII C'CTAG_G
Cuts at: 0 463 1200
Size: 463 737
BanI G'GyrC_C
Cuts at: 0 15 241 289 1200
Size: 15 226 48 911
Fragments arranged by size:
911 226 48 15
BanII G_rGCy'C
Cuts at: 0 178 653 761 1184 1200
Size: 178 475 108 423 16
Fragments arranged by size:
475 423 178 108 16
BbeI G_GCGC'C
Cuts at: 0 19 1200
Size: 19 1181
BbsI GAAGACnn'nnnn_
Cuts at: 0 1081 1136 1200
Size: 1081 55 64
Fragments arranged by size:
1081 64 55
BbvCI CC'TCA_GC
Cuts at: 0 991 1200
Size: 991 209
BciVI GTATCCnnnnn_n'
Cuts at: 0 561 1005 1200
Size: 561 444 195
Fragments arranged by size:
561 444 195
BglI GCCn_nnn'nGGC
Cuts at: 0 39 1200
Size: 39 1161
Bme1580I G_kGCm'C
Cuts at: 0 246 292 1200
Size: 246 46 908
Fragments arranged by size:
908 246 46
BmgBI CAC'GTC
Cuts at: 0 57 357 1200
Size: 57 300 843
Fragments arranged by size:
843 300 57
BmrI ACTGGGnnnn_n'
Cuts at: 0 596 814 1200
Size: 596 218 386
Fragments arranged by size:
596 386 218
Bpu10I CC'TnA_GC
Cuts at: 0 610 991 1200
Size: 610 381 209
Fragments arranged by size:
610 381 209
BsaHI Gr'CG_yC
Cuts at: 0 16 1200
Size: 16 1184
BsaWI w'CCGG_w
Cuts at: 0 978 1200
Size: 978 222
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 769 1200
Size: 769 431
BseYI C'CCAG_C
Cuts at: 0 235 245 285 386 554 1200
Size: 235 10 40 101 168 646
Fragments arranged by size:
646 235 168 101 40 10
BsiHKAI G_wGCw'C
Cuts at: 0 761 934 1200
Size: 761 173 266
Fragments arranged by size:
761 266 173
BsmI GAATG_Cn'
Cuts at: 0 749 1200
Size: 749 451
Bsp1286I G_dGCh'C
Cuts at: 0 178 246 292 653 761 934 1184 1200
Size: 178 68 46 361 108 173 250 16
Fragments arranged by size:
361 250 178 173 108 68 46 16
BspEI T'CCGG_A
Cuts at: 0 978 1200
Size: 978 222
BspHI T'CATG_A
Cuts at: 0 104 653 761 1200
Size: 104 549 108 439
Fragments arranged by size:
549 439 108 104
BsrBI CCG'CTC
Cuts at: 0 27 257 1200
Size: 27 230 943
Fragments arranged by size:
943 230 27
BsrDI GCAATG_nn'
Cuts at: 0 1002 1200
Size: 1002 198
BsrFI r'CCGG_y
Cuts at: 0 292 1200
Size: 292 908
BssSI C'ACGA_G
Cuts at: 0 123 565 1200
Size: 123 442 635
Fragments arranged by size:
635 442 123
BstAPI GCAn_nnn'nTGC
Cuts at: 0 702 1200
Size: 702 498
BstBI TT'CG_AA
Cuts at: 0 741 1200
Size: 741 459
BstYI r'GATC_y
Cuts at: 0 137 865 888 1200
Size: 137 728 23 312
Fragments arranged by size:
728 312 137 23
BtgI C'CryG_G
Cuts at: 0 542 797 1200
Size: 542 255 403
Fragments arranged by size:
542 403 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 487 1200
Size: 487 713
BtsI GCAGTG_nn'
Cuts at: 0 373 1200
Size: 373 827
EaeI y'GGCC_r
Cuts at: 0 213 404 411 1200
Size: 213 191 7 789
Fragments arranged by size:
789 213 191 7
EarI CTCTTCn'nnn_
Cuts at: 0 97 1150 1200
Size: 97 1053 50
Fragments arranged by size:
1053 97 50
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 144 328 1200
Size: 144 184 872
Fragments arranged by size:
872 184 144
EcoICRI GAG'CTC
Cuts at: 0 759 1200
Size: 759 441
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 1148 1200
Size: 694 454 52
Fragments arranged by size:
694 454 52
EcoO109I rG'GnC_Cy
Cuts at: 0 66 223 1200
Size: 66 157 977
Fragments arranged by size:
977 157 66
HaeII r_GCGC'y
Cuts at: 0 19 1200
Size: 19 1181
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 160 192 850 882 1144 1176 1200
Size: 160 32 658 32 262 32 24
Fragments arranged by size:
658 262 160 32 32 32 24
HincII GTy'rAC
Cuts at: 0 787 837 1107 1200
Size: 787 50 270 93
Fragments arranged by size:
787 270 93 50
KasI G'GCGC_C
Cuts at: 0 15 1200
Size: 15 1185
MscI TGG'CCA
Cuts at: 0 413 1200
Size: 413 787
MslI CAynn'nnrTG
Cuts at: 0 526 658 1200
Size: 526 132 542
Fragments arranged by size:
542 526 132
MspA1I CmG'CkG
Cuts at: 0 285 1200
Size: 285 915
NarI GG'CG_CC
Cuts at: 0 16 1200
Size: 16 1184
NcoI C'CATG_G
Cuts at: 0 542 797 1200
Size: 542 255 403
Fragments arranged by size:
542 403 255
NdeI CA'TA_TG
Cuts at: 0 494 1200
Size: 494 706
NspI r_CATG'y
Cuts at: 0 253 1200
Size: 253 947
PfoI T'CCnGG_A
Cuts at: 0 1092 1200
Size: 1092 108
PpuMI rG'GwC_Cy
Cuts at: 0 66 1200
Size: 66 1134
PstI C_TGCA'G
Cuts at: 0 284 1200
Size: 284 916
PvuII CAG'CTG
Cuts at: 0 285 1200
Size: 285 915
SacI G_AGCT'C
Cuts at: 0 761 1200
Size: 761 439
SalI G'TCGA_C
Cuts at: 0 785 1200
Size: 785 415
SfcI C'TryA_G
Cuts at: 0 280 960 1200
Size: 280 680 240
Fragments arranged by size:
680 280 240
SfoI GGC'GCC
Cuts at: 0 17 1200
Size: 17 1183
SgrAI Cr'CCGG_yG
Cuts at: 0 292 1200
Size: 292 908
SmaI CCC'GGG
Cuts at: 0 221 383 1200
Size: 221 162 817
Fragments arranged by size:
817 221 162
StyI C'CwwG_G
Cuts at: 0 463 542 797 1200
Size: 463 79 255 403
Fragments arranged by size:
463 403 255 79
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 376 858 1053 1200
Size: 376 482 195 147
Fragments arranged by size:
482 376 195 147
XmaI C'CCGG_G
Cuts at: 0 219 381 1200
Size: 219 162 819
Fragments arranged by size:
819 219 162
XmnI GAAnn'nnTTC
Cuts at: 0 737 1200
Size: 737 463
Enzymes that do cut:
AccI AclI AcuI AleI AlfI AlwNI AvaI AvrII
BanI BanII BbeI BbsI BbvCI BciVI BglI Bme1580I
BmgBI BmrI Bpu10I BsaHI BsaWI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI EaeI EarI
EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII KasI
MscI MslI MspA1I NarI NcoI NdeI NspI PfoI
PpuMI PstI PvuII SacI SalI SfcI SfoI SgrAI
SmaI StyI TaqII XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AloI ApaI ApaLI ApoI AscI AseI AsiSI BaeI
BamHI BcgI BclI BfrBI BglII BlpI BmtI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaXI BsgI BsiEI
BsiWI BsmBI BspMI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KpnI MfeI MluI MmeI NaeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SapI SbfI ScaI SexAI SfiI SgfI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI TstI Tth111I XbaI XcmI XhoI ZraI