Restriction map for
pF1KB5646
(Linear) (Six-base) MAPSORT of: KIBB5646.seq Check: 5479 from: 1 to: 1464
KIBB5646.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1277 1464
Size: 1277 187
AflII C'TTAA_G
Cuts at: 0 714 1464
Size: 714 750
AflIII A'CryG_T
Cuts at: 0 330 1464
Size: 330 1134
ApoI r'AATT_y
Cuts at: 0 182 389 700 1464
Size: 182 207 311 764
Fragments arranged by size:
764 311 207 182
AseI AT'TA_AT
Cuts at: 0 629 922 1464
Size: 629 293 542
Fragments arranged by size:
629 542 293
AvaI C'yCGr_G
Cuts at: 0 31 49 79 1448 1464
Size: 31 18 30 1369 16
Fragments arranged by size:
1369 31 30 18 16
BamHI G'GATC_C
Cuts at: 0 1050 1464
Size: 1050 414
BanI G'GyrC_C
Cuts at: 0 462 1464
Size: 462 1002
BanII G_rGCy'C
Cuts at: 0 226 1464
Size: 226 1238
BlpI GC'TnA_GC
Cuts at: 0 70 1464
Size: 70 1394
BmtI G_CTAG'C
Cuts at: 0 379 1464
Size: 379 1085
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 1464
Size: 126 1338
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 402 881 1464
Size: 308 94 479 583
Fragments arranged by size:
583 479 308 94
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 245 663 1464
Size: 245 418 801
Fragments arranged by size:
801 418 245
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 974 1004 1464
Size: 974 30 460
Fragments arranged by size:
974 460 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1099 1307 1464
Size: 1099 208 157
Fragments arranged by size:
1099 208 157
BseYI C'CCAG_C
Cuts at: 0 735 1464
Size: 735 729
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 747 824 1058 1464
Size: 747 77 234 406
Fragments arranged by size:
747 406 234 77
BsiHKAI G_wGCw'C
Cuts at: 0 71 1464
Size: 71 1393
Bsp1286I G_dGCh'C
Cuts at: 0 71 226 1464
Size: 71 155 1238
Fragments arranged by size:
1238 155 71
BsrBI CCG'CTC
Cuts at: 0 12 1464
Size: 12 1452
BsrFI r'CCGG_y
Cuts at: 0 1430 1464
Size: 1430 34
BsrGI T'GTAC_A
Cuts at: 0 813 1464
Size: 813 651
BssHII G'CGCG_C
Cuts at: 0 93 1464
Size: 93 1371
BstYI r'GATC_y
Cuts at: 0 1050 1198 1464
Size: 1050 148 266
Fragments arranged by size:
1050 266 148
Bsu36I CC'TnA_GG
Cuts at: 0 1412 1464
Size: 1412 52
BtsI GCAGTG_nn'
Cuts at: 0 759 1464
Size: 759 705
DraI TTT'AAA
Cuts at: 0 326 1464
Size: 326 1138
EaeI y'GGCC_r
Cuts at: 0 1117 1464
Size: 1117 347
EarI CTCTTCn'nnn_
Cuts at: 0 114 717 1464
Size: 114 603 747
Fragments arranged by size:
747 603 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1464
Size: 22 1442
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 402 881 1277 1464
Size: 308 94 479 396 187
Fragments arranged by size:
479 396 308 187 94
EcoNI CCTnn'n_nnAGG
Cuts at: 0 596 1415 1464
Size: 596 819 49
Fragments arranged by size:
819 596 49
EcoRV GAT'ATC
Cuts at: 0 494 1464
Size: 494 970
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 571 1464
Size: 571 893
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 287 319 1183 1215 1395 1427 1464
Size: 287 32 864 32 180 32 37
Fragments arranged by size:
864 287 180 37 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 724 1137 1464
Size: 431 293 413 327
Fragments arranged by size:
431 413 327 293
MscI TGG'CCA
Cuts at: 0 1119 1464
Size: 1119 345
MspA1I CmG'CkG
Cuts at: 0 74 1464
Size: 74 1390
NdeI CA'TA_TG
Cuts at: 0 471 1464
Size: 471 993
NheI G'CTAG_C
Cuts at: 0 375 1464
Size: 375 1089
PflMI CCAn_nnn'nTGG
Cuts at: 0 286 1464
Size: 286 1178
PfoI T'CCnGG_A
Cuts at: 0 143 1464
Size: 143 1321
PsiI TTA'TAA
Cuts at: 0 188 1464
Size: 188 1276
PstI C_TGCA'G
Cuts at: 0 118 1464
Size: 118 1346
SapI GCTCTTCn'nnn_
Cuts at: 0 114 1464
Size: 114 1350
SfcI C'TryA_G
Cuts at: 0 114 1464
Size: 114 1350
SmaI CCC'GGG
Cuts at: 0 33 51 81 1464
Size: 33 18 30 1383
Fragments arranged by size:
1383 33 30 18
SmlI C'TyrA_G
Cuts at: 0 260 678 714 1464
Size: 260 418 36 750
Fragments arranged by size:
750 418 260 36
StuI AGG'CCT
Cuts at: 0 1281 1464
Size: 1281 183
StyI C'CwwG_G
Cuts at: 0 575 1464
Size: 575 889
TatI w'GTAC_w
Cuts at: 0 652 813 1464
Size: 652 161 651
Fragments arranged by size:
652 651 161
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 738 770 1464
Size: 738 32 694
Fragments arranged by size:
738 694 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1239 1464
Size: 1239 225
XmaI C'CCGG_G
Cuts at: 0 31 49 79 1464
Size: 31 18 30 1385
Fragments arranged by size:
1385 31 30 18
XmnI GAAnn'nnTTC
Cuts at: 0 389 1464
Size: 389 1075
Enzymes that do cut:
AcuI AflII AflIII ApoI AseI AvaI BamHI BanI
BanII BlpI BmtI BplI BpmI BpuEI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BsrBI BsrFI BsrGI BssHII
BstYI Bsu36I BtsI DraI EaeI EarI EciI Eco57MI
EcoNI EcoRV FalI Hin4I MmeI MscI MspA1I NdeI
NheI PflMI PfoI PsiI PstI SapI SfcI SmaI
SmlI StuI StyI TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AsiSI
AvrII BaeI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BssSI BstAPI BstBI BstEII
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraIII DrdI
EagI EcoICRI EcoO109I EcoRI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MslI
NaeI NarI NcoI NgoMIV NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XhoI ZraI