(Linear) (Six-base) MAPSORT of: KIBB5635.seq  Check: 6717  from: 1  to: 1422



KIBB5635.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

BanI G'GyrC_C

Cuts at:      0     263    1422 
   Size:        263    1159
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     687    1422 
   Size:        687     735
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     738     772    1422 
   Size:        738      34     650

  Fragments arranged by size: 

                 738     650      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1094    1422 
   Size:       1094     328
 
BclI T'GATC_A

Cuts at:      0    1265    1422 
   Size:       1265     157
 
BfrBI ATG'CAT

Cuts at:      0     516    1422 
   Size:        516     906
 
Bme1580I G_kGCm'C

Cuts at:      0     268    1422 
   Size:        268    1154
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     713    1422 
   Size:        713     709
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1135    1422 
   Size:       1135     287
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1189    1422 
   Size:       1189     233
 
BsaBI GATnn'nnATC

Cuts at:      0    1270    1422 
   Size:       1270     152
 
BsaWI w'CCGG_w

Cuts at:      0     548    1422 
   Size:        548     874
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     179    1422 
   Size:        179    1243
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     291     464     552    1422 
   Size:        172     119     173      88     870

  Fragments arranged by size: 

                 870     173     172     119      88
 
BsiWI C'GTAC_G

Cuts at:      0     899    1422 
   Size:        899     523
 
BsmI GAATG_Cn'

Cuts at:      0     516    1149    1422 
   Size:        516     633     273

  Fragments arranged by size: 

                 633     516     273
 
Bsp1286I G_dGCh'C

Cuts at:      0     268    1422 
   Size:        268    1154
 
BspHI T'CATG_A

Cuts at:      0     777    1014    1422 
   Size:        777     237     408

  Fragments arranged by size: 

                 777     408     237
 
BsrBI CCG'CTC

Cuts at:      0     726    1422 
   Size:        726     696
 
BsrDI GCAATG_nn'

Cuts at:      0     284     435     696     801    1422 
   Size:        284     151     261     105     621

  Fragments arranged by size: 

                 621     284     261     151     105
 
BssSI C'ACGA_G

Cuts at:      0      89    1422 
   Size:         89    1333
 
BstBI TT'CG_AA

Cuts at:      0    1133    1422 
   Size:       1133     289
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     678    1422 
   Size:        678     744
 
BstYI r'GATC_y

Cuts at:      0     326    1200    1422 
   Size:        326     874     222

  Fragments arranged by size: 

                 874     326     222
 
BtsI GCAGTG_nn'

Cuts at:      0    1241    1422 
   Size:       1241     181
 
DraI TTT'AAA

Cuts at:      0    1334    1422 
   Size:       1334      88
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     310    1422 
   Size:        310    1112
 
EaeI y'GGCC_r

Cuts at:      0     231     680    1295    1422 
   Size:        231     449     615     127

  Fragments arranged by size: 

                 615     449     231     127
 
EarI CTCTTCn'nnn_

Cuts at:      0     779    1118    1422 
   Size:        779     339     304

  Fragments arranged by size: 

                 779     339     304
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1135    1422 
   Size:       1135     287
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     941    1422 
   Size:        941     481
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     386     418    1330    1362    1422 
   Size:        386      32     912      32      60

  Fragments arranged by size: 

                 912     386      60      32      32
 
HincII GTy'rAC

Cuts at:      0     981    1422 
   Size:        981     441
 
HpaI GTT'AAC

Cuts at:      0     981    1422 
   Size:        981     441
 
MfeI C'AATT_G

Cuts at:      0     767    1422 
   Size:        767     655
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     442    1301    1422 
   Size:        442     859     121

  Fragments arranged by size: 

                 859     442     121
 
MscI TGG'CCA

Cuts at:      0     233    1422 
   Size:        233    1189
 
MslI CAynn'nnrTG

Cuts at:      0     313    1422 
   Size:        313    1109
 
MspA1I CmG'CkG

Cuts at:      0     293     732    1096    1422 
   Size:        293     439     364     326

  Fragments arranged by size: 

                 439     364     326     293
 
NsiI A_TGCA'T

Cuts at:      0     518    1422 
   Size:        518     904
 
NspI r_CATG'y

Cuts at:      0      37     960    1422 
   Size:         37     923     462

  Fragments arranged by size: 

                 923     462      37
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     337    1422 
   Size:        337    1085
 
PsiI TTA'TAA

Cuts at:      0     499    1422 
   Size:        499     923
 
PvuII CAG'CTG

Cuts at:      0     732    1422 
   Size:        732     690
 
SapI GCTCTTCn'nnn_

Cuts at:      0     779    1422 
   Size:        779     643
 
SfcI C'TryA_G

Cuts at:      0     221    1422 
   Size:        221    1201
 
SmlI C'TyrA_G

Cuts at:      0    1204    1422 
   Size:       1204     218
 

 Enzymes that do cut:

     BanI     BbsI     BcgI    BciVI     BclI    BfrBI Bme1580I     BmrI
     BpmI    BpuEI    BsaBI    BsaWI    BseRI     BsgI    BsiWI     BsmI
 Bsp1286I    BspHI    BsrBI    BsrDI    BssSI    BstBI    BstXI    BstYI
     BtsI     DraI     DrdI     EaeI     EarI  Eco57MI    EcoNI    Hin4I
   HincII     HpaI     MfeI     MmeI     MscI     MslI   MspA1I     NsiI
     NspI    PflMI     PsiI    PvuII     SapI     SfcI     SmlI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
    BamHI    BanII     BbeI    BbvCI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaHI    BsaXI    BseYI
    BsiEI  BsiHKAI    BsmBI    BspEI    BspMI    BsrFI    BsrGI   BssHII
   BstAPI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     EagI     EciI  EcoICRI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII  HindIII     KasI     KpnI     MluI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     PacI     PasI     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI