Restriction map for
pF1KB5580
(Linear) (Six-base) MAPSORT of: KIBB5580.seq Check: 8098 from: 1 to: 1845
KIBB5580.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1415 1845
Size: 1415 430
AatII G_ACGT'C
Cuts at: 0 380 1845
Size: 380 1465
AccI GT'mk_AC
Cuts at: 0 1108 1845
Size: 1108 737
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1845
Size: 929 916
AflIII A'CryG_T
Cuts at: 0 1152 1845
Size: 1152 693
AleI CACnn'nnGTG
Cuts at: 0 175 736 1845
Size: 175 561 1109
Fragments arranged by size:
1109 561 175
ApaLI G'TGCA_C
Cuts at: 0 1204 1845
Size: 1204 641
ApoI r'AATT_y
Cuts at: 0 498 1603 1845
Size: 498 1105 242
Fragments arranged by size:
1105 498 242
AvaI C'yCGr_G
Cuts at: 0 986 1845
Size: 986 859
BamHI G'GATC_C
Cuts at: 0 190 323 1682 1845
Size: 190 133 1359 163
Fragments arranged by size:
1359 190 163 133
BanII G_rGCy'C
Cuts at: 0 76 876 1260 1334 1720 1845
Size: 76 800 384 74 386 125
Fragments arranged by size:
800 386 384 125 76 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 376 763 1667 1845
Size: 376 387 904 178
Fragments arranged by size:
904 387 376 178
BbvCI CC'TCA_GC
Cuts at: 0 59 1845
Size: 59 1786
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1240 1274 1845
Size: 1240 34 571
Fragments arranged by size:
1240 571 34
BfrBI ATG'CAT
Cuts at: 0 1534 1845
Size: 1534 311
BglI GCCn_nnn'nGGC
Cuts at: 0 1279 1845
Size: 1279 566
Bme1580I G_kGCm'C
Cuts at: 0 883 1208 1362 1845
Size: 883 325 154 483
Fragments arranged by size:
883 483 325 154
BmgBI CAC'GTC
Cuts at: 0 1089 1155 1845
Size: 1089 66 690
Fragments arranged by size:
1089 690 66
BmrI ACTGGGnnnn_n'
Cuts at: 0 248 373 1172 1263 1347 1845
Size: 248 125 799 91 84 498
Fragments arranged by size:
799 498 248 125 91 84
Bpu10I CC'TnA_GC
Cuts at: 0 59 1002 1845
Size: 59 943 843
Fragments arranged by size:
943 843 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1780 1845
Size: 1780 65
BsaI GGTCTCn'nnnn_
Cuts at: 0 1094 1756 1845
Size: 1094 662 89
Fragments arranged by size:
1094 662 89
BsaAI yAC'GTr
Cuts at: 0 817 1845
Size: 817 1028
BsaHI Gr'CG_yC
Cuts at: 0 182 377 1845
Size: 182 195 1468
Fragments arranged by size:
1468 195 182
BsaWI w'CCGG_w
Cuts at: 0 187 551 695 1445 1831 1845
Size: 187 364 144 750 386 14
Fragments arranged by size:
750 386 364 187 144 14
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 1845
Size: 412 30 1403
Fragments arranged by size:
1403 412 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1023 1088 1845
Size: 1023 65 757
Fragments arranged by size:
1023 757 65
BseYI C'CCAG_C
Cuts at: 0 1269 1845
Size: 1269 576
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 637 1845
Size: 637 1208
BsiEI CG_ry'CG
Cuts at: 0 435 1845
Size: 435 1410
BsiHKAI G_wGCw'C
Cuts at: 0 876 1208 1334 1469 1720 1845
Size: 876 332 126 135 251 125
Fragments arranged by size:
876 332 251 135 126 125
BsmI GAATG_Cn'
Cuts at: 0 65 1845
Size: 65 1780
Bsp1286I G_dGCh'C
Cuts at: 0 76 876 883 1208 1260 1334 1362 1469
Size: 76 800 7 325 52 74 28 107
Cuts at: 1469 1720 1845
Size: 251 125
Fragments arranged by size:
800 325 251 125 107 76 74 52
28 7
BspEI T'CCGG_A
Cuts at: 0 187 695 1845
Size: 187 508 1150
Fragments arranged by size:
1150 508 187
BspHI T'CATG_A
Cuts at: 0 1367 1845
Size: 1367 478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 90 1415 1845
Size: 90 1325 430
Fragments arranged by size:
1325 430 90
BsrDI GCAATG_nn'
Cuts at: 0 1767 1845
Size: 1767 78
BsrFI r'CCGG_y
Cuts at: 0 572 1698 1845
Size: 572 1126 147
Fragments arranged by size:
1126 572 147
BstEII G'GTnAC_C
Cuts at: 0 1441 1845
Size: 1441 404
BstXI CCAn_nnnn'nTGG
Cuts at: 0 738 1386 1845
Size: 738 648 459
Fragments arranged by size:
738 648 459
BstYI r'GATC_y
Cuts at: 0 190 323 1682 1845
Size: 190 133 1359 163
Fragments arranged by size:
1359 190 163 133
BtgI C'CryG_G
Cuts at: 0 842 1845
Size: 842 1003
BtsI GCAGTG_nn'
Cuts at: 0 182 1845
Size: 182 1663
DrdI GACnn_nn'nnGTC
Cuts at: 0 310 767 1153 1845
Size: 310 457 386 692
Fragments arranged by size:
692 457 386 310
EaeI y'GGCC_r
Cuts at: 0 432 839 1821 1845
Size: 432 407 982 24
Fragments arranged by size:
982 432 407 24
EagI C'GGCC_G
Cuts at: 0 432 1845
Size: 432 1413
EarI CTCTTCn'nnn_
Cuts at: 0 1041 1339 1845
Size: 1041 298 506
Fragments arranged by size:
1041 506 298
EcoICRI GAG'CTC
Cuts at: 0 874 1332 1718 1845
Size: 874 458 386 127
Fragments arranged by size:
874 458 386 127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 929 1845
Size: 929 916
EcoO109I rG'GnC_Cy
Cuts at: 0 1132 1845
Size: 1132 713
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 941 1450 1845
Size: 941 509 395
Fragments arranged by size:
941 509 395
HaeII r_GCGC'y
Cuts at: 0 278 1845
Size: 278 1567
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 143 175 331 363 412 444 711 743
Size: 143 32 156 32 49 32 267 32
Cuts at: 743 952 984 1018 1050 1067 1099 1210 1242
Size: 209 32 34 32 17 32 111 32
Cuts at: 1242 1845
Size: 603
Fragments arranged by size:
603 267 209 156 143 111 49 34
32 32 32 32 32 32 32 32
17
HincII GTy'rAC
Cuts at: 0 1109 1401 1845
Size: 1109 292 444
Fragments arranged by size:
1109 444 292
HindIII A'AGCT_T
Cuts at: 0 1044 1845
Size: 1044 801
MfeI C'AATT_G
Cuts at: 0 663 1845
Size: 663 1182
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1745 1845
Size: 1745 100
MscI TGG'CCA
Cuts at: 0 841 1823 1845
Size: 841 982 22
Fragments arranged by size:
982 841 22
MslI CAynn'nnrTG
Cuts at: 0 175 604 736 1845
Size: 175 429 132 1109
Fragments arranged by size:
1109 429 175 132
MspA1I CmG'CkG
Cuts at: 0 384 512 560 1845
Size: 384 128 48 1285
Fragments arranged by size:
1285 384 128 48
NaeI GCC'GGC
Cuts at: 0 574 1700 1845
Size: 574 1126 145
Fragments arranged by size:
1126 574 145
NgoMIV G'CCGG_C
Cuts at: 0 572 1698 1845
Size: 572 1126 147
Fragments arranged by size:
1126 572 147
NsiI A_TGCA'T
Cuts at: 0 1536 1845
Size: 1536 309
PfoI T'CCnGG_A
Cuts at: 0 1421 1845
Size: 1421 424
PshAI GACnn'nnGTC
Cuts at: 0 376 1845
Size: 376 1469
PstI C_TGCA'G
Cuts at: 0 1412 1484 1845
Size: 1412 72 361
Fragments arranged by size:
1412 361 72
PvuII CAG'CTG
Cuts at: 0 512 1845
Size: 512 1333
SacI G_AGCT'C
Cuts at: 0 876 1334 1720 1845
Size: 876 458 386 125
Fragments arranged by size:
876 458 386 125
SalI G'TCGA_C
Cuts at: 0 1107 1845
Size: 1107 738
SapI GCTCTTCn'nnn_
Cuts at: 0 1339 1845
Size: 1339 506
ScaI AGT'ACT
Cuts at: 0 403 1845
Size: 403 1442
SfcI C'TryA_G
Cuts at: 0 47 507 1408 1480 1547 1845
Size: 47 460 901 72 67 298
Fragments arranged by size:
901 460 298 72 67 47
SmlI C'TyrA_G
Cuts at: 0 1795 1845
Size: 1795 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1128 1250 1845
Size: 1128 122 595
Fragments arranged by size:
1128 595 122
TatI w'GTAC_w
Cuts at: 0 401 1845
Size: 401 1444
XmnI GAAnn'nnTTC
Cuts at: 0 634 1845
Size: 634 1211
ZraI GAC'GTC
Cuts at: 0 378 1845
Size: 378 1467
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI ApaLI ApoI
AvaI BamHI BanII BbsI BbvCI BcgI BfrBI BglI
Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrDI BsrFI BstEII BstXI
BstYI BtgI BtsI DrdI EaeI EagI EarI EcoICRI
Eco57MI EcoO109I FalI HaeII Hin4I HincII HindIII MfeI
MmeI MscI MslI MspA1I NaeI NgoMIV NsiI PfoI
PshAI PstI PvuII SacI SalI SapI ScaI SfcI
SmlI TaqII TatI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI
AlwNI ApaI AscI AseI AsiSI AvrII BaeI BanI
BbeI BciVI BclI BglII BlpI BmtI BplI BpmI
BsaBI BsiWI BsmBI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EciI EcoNI EcoRI EcoRV FseI FspI FspAI HpaI
KasI KpnI MluI NarI NcoI NdeI NheI NotI
NruI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SanDI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI