Restriction map for
pF1KB5567
(Linear) (Six-base) MAPSORT of: KIBB5567.seq Check: 3254 from: 1 to: 2016
KIBB5567.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1040 1189 2016
Size: 1040 149 827
Fragments arranged by size:
1040 827 149
AfeI AGC'GCT
Cuts at: 0 1618 2016
Size: 1618 398
AflII C'TTAA_G
Cuts at: 0 1128 2016
Size: 1128 888
AflIII A'CryG_T
Cuts at: 0 129 2016
Size: 129 1887
AlwNI CAG_nnn'CTG
Cuts at: 0 1033 1597 2016
Size: 1033 564 419
Fragments arranged by size:
1033 564 419
ApaI G_GGCC'C
Cuts at: 0 1513 2016
Size: 1513 503
ApaLI G'TGCA_C
Cuts at: 0 926 2016
Size: 926 1090
ApoI r'AATT_y
Cuts at: 0 1136 1464 1651 1978 2016
Size: 1136 328 187 327 38
Fragments arranged by size:
1136 328 327 187 38
BanI G'GyrC_C
Cuts at: 0 705 2016
Size: 705 1311
BanII G_rGCy'C
Cuts at: 0 467 1513 1553 2016
Size: 467 1046 40 463
Fragments arranged by size:
1046 467 463 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 838 1046 1242 1430 2016
Size: 838 208 196 188 586
Fragments arranged by size:
838 586 208 196 188
BglII A'GATC_T
Cuts at: 0 4 2016
Size: 4 2012
Bme1580I G_kGCm'C
Cuts at: 0 708 930 1513 2016
Size: 708 222 583 503
Fragments arranged by size:
708 583 503 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 1240 2016
Size: 1240 776
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1467 1877 2016
Size: 997 470 410 139
Fragments arranged by size:
997 470 410 139
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 810 1431 1558 2016
Size: 810 621 127 458
Fragments arranged by size:
810 621 458 127
BsaI GGTCTCn'nnnn_
Cuts at: 0 337 860 1576 2016
Size: 337 523 716 440
Fragments arranged by size:
716 523 440 337
BsaAI yAC'GTr
Cuts at: 0 1326 2016
Size: 1326 690
BsaBI GATnn'nnATC
Cuts at: 0 645 2016
Size: 645 1371
BsaHI Gr'CG_yC
Cuts at: 0 598 2016
Size: 598 1418
BsaWI w'CCGG_w
Cuts at: 0 72 558 2016
Size: 72 486 1458
Fragments arranged by size:
1458 486 72
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 524 554 1705 1735 1844 1860 1874 1890
Size: 524 30 1151 30 109 16 14 16
Cuts at: 1890 2016
Size: 126
Fragments arranged by size:
1151 524 126 109 30 30 16 16
14
BseYI C'CCAG_C
Cuts at: 0 778 1048 2016
Size: 778 270 968
Fragments arranged by size:
968 778 270
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1715 2016
Size: 1715 301
BsiHKAI G_wGCw'C
Cuts at: 0 123 930 1553 2016
Size: 123 807 623 463
Fragments arranged by size:
807 623 463 123
BsmI GAATG_Cn'
Cuts at: 0 668 1173 2016
Size: 668 505 843
Fragments arranged by size:
843 668 505
BsmBI CGTCTCn'nnnn_
Cuts at: 0 605 786 2016
Size: 605 181 1230
Fragments arranged by size:
1230 605 181
Bsp1286I G_dGCh'C
Cuts at: 0 123 467 708 930 1513 1553 2016
Size: 123 344 241 222 583 40 463
Fragments arranged by size:
583 463 344 241 222 123 40
BspEI T'CCGG_A
Cuts at: 0 72 558 2016
Size: 72 486 1458
Fragments arranged by size:
1458 486 72
BspHI T'CATG_A
Cuts at: 0 849 2016
Size: 849 1167
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1654 2016
Size: 1654 362
BsrDI GCAATG_nn'
Cuts at: 0 1355 2016
Size: 1355 661
BstAPI GCAn_nnn'nTGC
Cuts at: 0 934 2016
Size: 934 1082
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1956 2016
Size: 1956 60
BstYI r'GATC_y
Cuts at: 0 4 552 2016
Size: 4 548 1464
Fragments arranged by size:
1464 548 4
Bsu36I CC'TnA_GG
Cuts at: 0 1293 1769 2016
Size: 1293 476 247
Fragments arranged by size:
1293 476 247
EaeI y'GGCC_r
Cuts at: 0 721 2016
Size: 721 1295
EarI CTCTTCn'nnn_
Cuts at: 0 395 608 1560 1827 2016
Size: 395 213 952 267 189
Fragments arranged by size:
952 395 267 213 189
EcoICRI GAG'CTC
Cuts at: 0 1551 2016
Size: 1551 465
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 997 1040 1189 1467 1877 2016
Size: 997 43 149 278 410 139
Fragments arranged by size:
997 410 278 149 139 43
EcoO109I rG'GnC_Cy
Cuts at: 0 838 1509 1510 2016
Size: 838 671 1 506
Fragments arranged by size:
838 671 506 1
EcoRV GAT'ATC
Cuts at: 0 1357 2016
Size: 1357 659
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 388 1871 2016
Size: 388 1483 145
Fragments arranged by size:
1483 388 145
HaeII r_GCGC'y
Cuts at: 0 779 1620 2016
Size: 779 841 396
Fragments arranged by size:
841 779 396
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 537 569 1170 1202 1390 1422 1752 1784
Size: 537 32 601 32 188 32 330 32
Cuts at: 1784 1860 1892 2016
Size: 76 32 124
Fragments arranged by size:
601 537 330 188 124 76 32 32
32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 217 1384 2008 2016
Size: 125 92 1167 624 8
Fragments arranged by size:
1167 624 125 92 8
MscI TGG'CCA
Cuts at: 0 723 2016
Size: 723 1293
MspA1I CmG'CkG
Cuts at: 0 1795 1873 1927 2016
Size: 1795 78 54 89
Fragments arranged by size:
1795 89 78 54
NdeI CA'TA_TG
Cuts at: 0 1284 1911 2016
Size: 1284 627 105
Fragments arranged by size:
1284 627 105
NspI r_CATG'y
Cuts at: 0 133 613 937 2016
Size: 133 480 324 1079
Fragments arranged by size:
1079 480 324 133
PciI A'CATG_T
Cuts at: 0 129 2016
Size: 129 1887
PflMI CCAn_nnn'nTGG
Cuts at: 0 42 1900 2016
Size: 42 1858 116
Fragments arranged by size:
1858 116 42
PpuMI rG'GwC_Cy
Cuts at: 0 838 2016
Size: 838 1178
PsiI TTA'TAA
Cuts at: 0 445 2016
Size: 445 1571
PspOMI G'GGCC_C
Cuts at: 0 1509 2016
Size: 1509 507
PstI C_TGCA'G
Cuts at: 0 1806 2016
Size: 1806 210
PvuII CAG'CTG
Cuts at: 0 1795 1873 1927 2016
Size: 1795 78 54 89
Fragments arranged by size:
1795 89 78 54
SacI G_AGCT'C
Cuts at: 0 1553 2016
Size: 1553 463
SfcI C'TryA_G
Cuts at: 0 749 1262 1802 2016
Size: 749 513 540 214
Fragments arranged by size:
749 540 513 214
SmlI C'TyrA_G
Cuts at: 0 789 1128 1446 1573 2016
Size: 789 339 318 127 443
Fragments arranged by size:
789 443 339 318 127
SphI G_CATG'C
Cuts at: 0 937 2016
Size: 937 1079
StuI AGG'CCT
Cuts at: 0 787 1197 2016
Size: 787 410 819
Fragments arranged by size:
819 787 410
StyI C'CwwG_G
Cuts at: 0 221 593 1709 2016
Size: 221 372 1116 307
Fragments arranged by size:
1116 372 307 221
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 231 263 2016
Size: 231 32 1753
Fragments arranged by size:
1753 231 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 716 2016
Size: 716 1300
XmnI GAAnn'nnTTC
Cuts at: 0 89 2016
Size: 89 1927
Enzymes that do cut:
AcuI AfeI AflII AflIII AlwNI ApaI ApaLI ApoI
BanI BanII BbsI BglII Bme1580I BmrI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI
BstAPI BstXI BstYI Bsu36I EaeI EarI EcoICRI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I MmeI MscI MspA1I
NdeI NspI PciI PflMI PpuMI PsiI PspOMI PstI
PvuII SacI SfcI SmlI SphI StuI StyI TstI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AleI
AlfI AloI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BclI BfrBI BglI
BlpI BmgBI BmtI BplI Bpu10I BseRI BsiEI BsiWI
BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PfoI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SrfI SspI SwaI TaqII TatI Tth111I XbaI XhoI
XmaI ZraI