Restriction map for
pF1KB5562
(Linear) (Six-base) MAPSORT of: KIBB5562.seq Check: 1148 from: 1 to: 2361
KIBB5562.orf
With 182 enzymes: SgfI *
February 28, 2007 11:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 943 1935 2361
Size: 943 992 426
Fragments arranged by size:
992 943 426
Acc65I G'GTAC_C
Cuts at: 0 345 1536 2361
Size: 345 1191 825
Fragments arranged by size:
1191 825 345
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1226 2004 2361
Size: 665 561 778 357
Fragments arranged by size:
778 665 561 357
AflIII A'CryG_T
Cuts at: 0 2223 2361
Size: 2223 138
AleI CACnn'nnGTG
Cuts at: 0 1123 1136 1387 2361
Size: 1123 13 251 974
Fragments arranged by size:
1123 974 251 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 263 2361
Size: 263 2098
AlwNI CAG_nnn'CTG
Cuts at: 0 489 832 985 1843 2361
Size: 489 343 153 858 518
Fragments arranged by size:
858 518 489 343 153
ApaLI G'TGCA_C
Cuts at: 0 637 2361
Size: 637 1724
ApoI r'AATT_y
Cuts at: 0 86 172 1192 1794 2323 2361
Size: 86 86 1020 602 529 38
Fragments arranged by size:
1020 602 529 86 86 38
BanI G'GyrC_C
Cuts at: 0 345 1497 1536 2041 2361
Size: 345 1152 39 505 320
Fragments arranged by size:
1152 505 345 320 39
BanII G_rGCy'C
Cuts at: 0 247 775 1486 2361
Size: 247 528 711 875
Fragments arranged by size:
875 711 528 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 743 1214 2259 2361
Size: 743 471 1045 102
Fragments arranged by size:
1045 743 471 102
BciVI GTATCCnnnnn_n'
Cuts at: 0 715 2361
Size: 715 1646
BclI T'GATC_A
Cuts at: 0 192 480 630 2361
Size: 192 288 150 1731
Fragments arranged by size:
1731 288 192 150
BglI GCCn_nnn'nGGC
Cuts at: 0 2178 2361
Size: 2178 183
BlpI GC'TnA_GC
Cuts at: 0 844 2361
Size: 844 1517
Bme1580I G_kGCm'C
Cuts at: 0 363 641 2046 2361
Size: 363 278 1405 315
Fragments arranged by size:
1405 363 315 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 58 820 1977 2361
Size: 58 762 1157 384
Fragments arranged by size:
1157 762 384 58
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 261 1107 1500 2361
Size: 261 846 393 861
Fragments arranged by size:
861 846 393 261
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1495 1704 2361
Size: 1495 209 657
Fragments arranged by size:
1495 657 209
Bpu10I CC'TnA_GC
Cuts at: 0 1485 1926 2361
Size: 1485 441 435
Fragments arranged by size:
1485 441 435
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 759 1645 2361
Size: 759 886 716
Fragments arranged by size:
886 759 716
BsaBI GATnn'nnATC
Cuts at: 0 191 1658 2361
Size: 191 1467 703
Fragments arranged by size:
1467 703 191
BsaWI w'CCGG_w
Cuts at: 0 1280 1286 2361
Size: 1280 6 1075
Fragments arranged by size:
1280 1075 6
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 92 122 1026 1056 1755 1785 2266 2296
Size: 92 30 904 30 699 30 481 30
Cuts at: 2296 2361
Size: 65
Fragments arranged by size:
904 699 481 92 65 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 113 1707 2361
Size: 113 1594 654
Fragments arranged by size:
1594 654 113
BseYI C'CCAG_C
Cuts at: 0 948 1940 2289 2361
Size: 948 992 349 72
Fragments arranged by size:
992 948 349 72
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 906 2361
Size: 906 1455
BsiEI CG_ry'CG
Cuts at: 0 1786 2361
Size: 1786 575
BsiHKAI G_wGCw'C
Cuts at: 0 247 641 2361
Size: 247 394 1720
Fragments arranged by size:
1720 394 247
Bsp1286I G_dGCh'C
Cuts at: 0 247 363 641 775 1486 2046 2361
Size: 247 116 278 134 711 560 315
Fragments arranged by size:
711 560 315 278 247 134 116
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 943 1020 1935 2361
Size: 943 77 915 426
Fragments arranged by size:
943 915 426 77
BsrBI CCG'CTC
Cuts at: 0 216 1925 2361
Size: 216 1709 436
Fragments arranged by size:
1709 436 216
BsrDI GCAATG_nn'
Cuts at: 0 59 2361
Size: 59 2302
BsrFI r'CCGG_y
Cuts at: 0 1785 2361
Size: 1785 576
BsrGI T'GTAC_A
Cuts at: 0 1051 2361
Size: 1051 1310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1004 2361
Size: 1004 1357
BstYI r'GATC_y
Cuts at: 0 711 1659 2095 2361
Size: 711 948 436 266
Fragments arranged by size:
948 711 436 266
Bsu36I CC'TnA_GG
Cuts at: 0 2261 2361
Size: 2261 100
BtgI C'CryG_G
Cuts at: 0 1411 2361
Size: 1411 950
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2039 2361
Size: 2039 322
BtsI GCAGTG_nn'
Cuts at: 0 918 2361
Size: 918 1443
DraIII CAC_nnn'GTG
Cuts at: 0 909 2361
Size: 909 1452
EaeI y'GGCC_r
Cuts at: 0 987 1013 1556 1783 2170 2253 2361
Size: 987 26 543 227 387 83 108
Fragments arranged by size:
987 543 387 227 108 83 26
EagI C'GGCC_G
Cuts at: 0 1783 2361
Size: 1783 578
EarI CTCTTCn'nnn_
Cuts at: 0 1539 2361
Size: 1539 822
EcoICRI GAG'CTC
Cuts at: 0 245 2361
Size: 245 2116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 665 1226 1495 1704 2004 2361
Size: 665 561 269 209 300 357
Fragments arranged by size:
665 561 357 300 269 209
EcoO109I rG'GnC_Cy
Cuts at: 0 1453 2361
Size: 1453 908
EcoRI G'AATT_C
Cuts at: 0 86 1192 2323 2361
Size: 86 1106 1131 38
Fragments arranged by size:
1131 1106 86 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 280 2361
Size: 280 2081
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 92 124 184 216 2266 2298
Size: 28 32 32 32 60 32 2050 32
Cuts at: 2298 2361
Size: 63
Fragments arranged by size:
2050 63 60 32 32 32 32 32
28
HincII GTy'rAC
Cuts at: 0 1075 2361
Size: 1075 1286
KpnI G_GTAC'C
Cuts at: 0 349 1540 2361
Size: 349 1191 821
Fragments arranged by size:
1191 821 349
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 1260 1451 1904 2361
Size: 209 1051 191 453 457
Fragments arranged by size:
1051 457 453 209 191
MscI TGG'CCA
Cuts at: 0 989 1015 1558 2172 2255 2361
Size: 989 26 543 614 83 106
Fragments arranged by size:
989 614 543 106 83 26
MslI CAynn'nnrTG
Cuts at: 0 644 760 1123 1136 1387 2361
Size: 644 116 363 13 251 974
Fragments arranged by size:
974 644 363 251 116 13
MspA1I CmG'CkG
Cuts at: 0 1136 1494 2361
Size: 1136 358 867
Fragments arranged by size:
1136 867 358
NaeI GCC'GGC
Cuts at: 0 1787 2361
Size: 1787 574
NgoMIV G'CCGG_C
Cuts at: 0 1785 2361
Size: 1785 576
NspI r_CATG'y
Cuts at: 0 2227 2361
Size: 2227 134
PasI CC'CwG_GG
Cuts at: 0 1755 2361
Size: 1755 606
PciI A'CATG_T
Cuts at: 0 2223 2361
Size: 2223 138
PflMI CCAn_nnn'nTGG
Cuts at: 0 985 1947 2361
Size: 985 962 414
Fragments arranged by size:
985 962 414
PfoI T'CCnGG_A
Cuts at: 0 707 2270 2361
Size: 707 1563 91
Fragments arranged by size:
1563 707 91
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1333 1365 2361
Size: 1333 32 996
Fragments arranged by size:
1333 996 32
PpuMI rG'GwC_Cy
Cuts at: 0 1453 2361
Size: 1453 908
PstI C_TGCA'G
Cuts at: 0 1848 2130 2361
Size: 1848 282 231
Fragments arranged by size:
1848 282 231
PvuII CAG'CTG
Cuts at: 0 1494 2361
Size: 1494 867
SacI G_AGCT'C
Cuts at: 0 247 2361
Size: 247 2114
SfcI C'TryA_G
Cuts at: 0 354 1844 2126 2361
Size: 354 1490 282 235
Fragments arranged by size:
1490 354 282 235
SmlI C'TyrA_G
Cuts at: 0 774 1624 2361
Size: 774 850 737
Fragments arranged by size:
850 774 737
SpeI A'CTAG_T
Cuts at: 0 1036 2361
Size: 1036 1325
SspI AAT'ATT
Cuts at: 0 1185 2361
Size: 1185 1176
StuI AGG'CCT
Cuts at: 0 940 2361
Size: 940 1421
StyI C'CwwG_G
Cuts at: 0 1890 2361
Size: 1890 471
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 656 1515 1753 1903 2152 2361
Size: 656 859 238 150 249 209
Fragments arranged by size:
859 656 249 238 209 150
TatI w'GTAC_w
Cuts at: 0 1051 1364 2361
Size: 1051 313 997
Fragments arranged by size:
1051 997 313
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 403 435 1755 1787 2361
Size: 403 32 1320 32 574
Fragments arranged by size:
1320 574 403 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 396 605 2361
Size: 396 209 1756
Fragments arranged by size:
1756 396 209
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaLI
ApoI BanI BanII BbsI BciVI BclI BglI BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EarI EcoICRI Eco57MI
EcoO109I EcoRI FalI Hin4I HincII KpnI MmeI MscI
MslI MspA1I NaeI NgoMIV NspI PasI PciI PflMI
PfoI PpiI PpuMI PstI PvuII SacI SfcI SmlI
SpeI SspI StuI StyI TaqII TatI TstI XcmI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BfrBI BglII BmgBI BmtI BsaI
BsaAI BsaHI BsiWI BsmI BsmBI BspEI BspHI BssHII
BssSI BstBI BstEII BstXI BstZ17I ClaI CspCI DraI
DrdI EciI EcoNI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI NarI NcoI NdeI
NheI NotI NruI NsiI PacI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SphI SrfI SwaI Tth111I XbaI
XhoI XmaI XmnI ZraI