(Linear) (Six-base) MAPSORT of: KIBB5559.seq  Check: 7368  from: 1  to: 735



KIBB5559.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     431     735 
   Size:        431     304
 
Acc65I G'GTAC_C

Cuts at:      0     202     735 
   Size:        202     533
 
ApoI r'AATT_y

Cuts at:      0     641     735 
   Size:        641      94
 
BanI G'GyrC_C

Cuts at:      0     202     223     735 
   Size:        202      21     512

  Fragments arranged by size: 

                 512     202      21
 
Bme1580I G_kGCm'C

Cuts at:      0     226     735 
   Size:        226     509
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     735 
   Size:        157     578
 
BsaBI GATnn'nnATC

Cuts at:      0     676     735 
   Size:        676      59
 
BsaHI Gr'CG_yC

Cuts at:      0       4     735 
   Size:          4     731
 
BsiHKAI G_wGCw'C

Cuts at:      0     191     735 
   Size:        191     544
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      17     735 
   Size:         17     718
 
Bsp1286I G_dGCh'C

Cuts at:      0     191     226     735 
   Size:        191      35     509

  Fragments arranged by size: 

                 509     191      35
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     253     735 
   Size:        253     482
 
BsrDI GCAATG_nn'

Cuts at:      0     191     287     735 
   Size:        191      96     448

  Fragments arranged by size: 

                 448     191      96
 
BssSI C'ACGA_G

Cuts at:      0     701     735 
   Size:        701      34
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     673     735 
   Size:        673      62
 
BstYI r'GATC_y

Cuts at:      0     437     735 
   Size:        437     298
 
DraI TTT'AAA

Cuts at:      0     158     735 
   Size:        158     577
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     735 
   Size:        157     578
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     378     735 
   Size:        378     357
 
KpnI G_GTAC'C

Cuts at:      0     206     735 
   Size:        206     529
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     690     735 
   Size:        690      45
 
MslI CAynn'nnrTG

Cuts at:      0     458     735 
   Size:        458     277
 
PsiI TTA'TAA

Cuts at:      0     595     735 
   Size:        595     140
 
PstI C_TGCA'G

Cuts at:      0      26     735 
   Size:         26     709
 
SfcI C'TryA_G

Cuts at:      0      22     404     735 
   Size:         22     382     331

  Fragments arranged by size: 

                 382     331      22
 
TatI w'GTAC_w

Cuts at:      0     547     735 
   Size:        547     188
 

 Enzymes that do cut:

     AccI   Acc65I     ApoI     BanI Bme1580I     BpmI    BsaBI    BsaHI
  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrDI    BssSI    BstXI    BstYI
     DraI  Eco57MI     FalI     KpnI     MmeI     MslI     PsiI     PstI
     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII
     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI    BsiWI
     BsmI    BspEI    BspHI    BsrBI    BsrFI    BsrGI   BssHII   BstAPI
    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     MfeI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI