Restriction map for
pF1KB5557
(Linear) (Six-base) MAPSORT of: KIBB5557.seq Check: 1735 from: 1 to: 1392
KIBB5557.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1224 1392
Size: 1224 168
AfeI AGC'GCT
Cuts at: 0 1199 1282 1392
Size: 1199 83 110
Fragments arranged by size:
1199 110 83
AflIII A'CryG_T
Cuts at: 0 452 478 1392
Size: 452 26 914
Fragments arranged by size:
914 452 26
AleI CACnn'nnGTG
Cuts at: 0 1126 1392
Size: 1126 266
ApaI G_GGCC'C
Cuts at: 0 213 1392
Size: 213 1179
ApoI r'AATT_y
Cuts at: 0 641 754 1392
Size: 641 113 638
Fragments arranged by size:
641 638 113
AvaI C'yCGr_G
Cuts at: 0 902 1392
Size: 902 490
BanI G'GyrC_C
Cuts at: 0 406 1392
Size: 406 986
BanII G_rGCy'C
Cuts at: 0 10 213 887 944 1392
Size: 10 203 674 57 448
Fragments arranged by size:
674 448 203 57 10
BbsI GAAGACnn'nnnn_
Cuts at: 0 125 1392
Size: 125 1267
BciVI GTATCCnnnnn_n'
Cuts at: 0 217 442 1392
Size: 217 225 950
Fragments arranged by size:
950 225 217
BglI GCCn_nnn'nGGC
Cuts at: 0 272 1392
Size: 272 1120
Bme1580I G_kGCm'C
Cuts at: 0 213 553 748 1392
Size: 213 340 195 644
Fragments arranged by size:
644 340 213 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 810 871 1054 1172 1392
Size: 519 291 61 183 118 220
Fragments arranged by size:
519 291 220 183 118 61
Bpu10I CC'TnA_GC
Cuts at: 0 1207 1392
Size: 1207 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 1359 1392
Size: 200 1159 33
Fragments arranged by size:
1159 200 33
BsaI GGTCTCn'nnnn_
Cuts at: 0 692 1392
Size: 692 700
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 403 433 1392
Size: 403 30 959
Fragments arranged by size:
959 403 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1015 1392
Size: 1015 377
BseYI C'CCAG_C
Cuts at: 0 79 1392
Size: 79 1313
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 971 974 1392
Size: 971 3 418
Fragments arranged by size:
971 418 3
BsiEI CG_ry'CG
Cuts at: 0 507 1392
Size: 507 885
BsiHKAI G_wGCw'C
Cuts at: 0 172 826 887 1392
Size: 172 654 61 505
Fragments arranged by size:
654 505 172 61
BsmI GAATG_Cn'
Cuts at: 0 1085 1392
Size: 1085 307
Bsp1286I G_dGCh'C
Cuts at: 0 10 172 213 553 748 826 887 944
Size: 10 162 41 340 195 78 61 57
Cuts at: 944 1392
Size: 448
Fragments arranged by size:
448 340 195 162 78 61 57 41
10
BsrBI CCG'CTC
Cuts at: 0 468 503 1392
Size: 468 35 889
Fragments arranged by size:
889 468 35
BsrDI GCAATG_nn'
Cuts at: 0 569 1392
Size: 569 823
BsrFI r'CCGG_y
Cuts at: 0 506 1064 1392
Size: 506 558 328
Fragments arranged by size:
558 506 328
BstYI r'GATC_y
Cuts at: 0 707 1392
Size: 707 685
BtgI C'CryG_G
Cuts at: 0 163 1124 1392
Size: 163 961 268
Fragments arranged by size:
961 268 163
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 369 1392
Size: 369 1023
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 765 800 1392
Size: 765 35 592
Fragments arranged by size:
765 592 35
DraIII CAC_nnn'GTG
Cuts at: 0 1099 1392
Size: 1099 293
DrdI GACnn_nn'nnGTC
Cuts at: 0 127 1392
Size: 127 1265
EaeI y'GGCC_r
Cuts at: 0 504 508 1392
Size: 504 4 884
Fragments arranged by size:
884 504 4
EagI C'GGCC_G
Cuts at: 0 504 1392
Size: 504 888
EarI CTCTTCn'nnn_
Cuts at: 0 297 618 1343 1392
Size: 297 321 725 49
Fragments arranged by size:
725 321 297 49
EcoICRI GAG'CTC
Cuts at: 0 885 1392
Size: 885 507
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 810 871 1054 1172 1224 1392
Size: 519 291 61 183 118 52 168
Fragments arranged by size:
519 291 183 168 118 61 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 218 1392
Size: 218 1174
EcoO109I rG'GnC_Cy
Cuts at: 0 209 210 1392
Size: 209 1 1182
Fragments arranged by size:
1182 209 1
EcoRI G'AATT_C
Cuts at: 0 641 1392
Size: 641 751
FseI GG_CCGG'CC
Cuts at: 0 510 1392
Size: 510 882
HaeII r_GCGC'y
Cuts at: 0 1201 1284 1392
Size: 1201 83 108
Fragments arranged by size:
1201 108 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1170 1202 1392
Size: 1170 32 190
Fragments arranged by size:
1170 190 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 707 941 1392
Size: 707 234 451
Fragments arranged by size:
707 451 234
MslI CAynn'nnrTG
Cuts at: 0 181 481 1123 1126 1392
Size: 181 300 642 3 266
Fragments arranged by size:
642 300 266 181 3
MspA1I CmG'CkG
Cuts at: 0 988 1392
Size: 988 404
NaeI GCC'GGC
Cuts at: 0 508 1392
Size: 508 884
NgoMIV G'CCGG_C
Cuts at: 0 506 1392
Size: 506 886
NspI r_CATG'y
Cuts at: 0 482 1392
Size: 482 910
PasI CC'CwG_GG
Cuts at: 0 240 1392
Size: 240 1152
PciI A'CATG_T
Cuts at: 0 478 1392
Size: 478 914
PflMI CCAn_nnn'nTGG
Cuts at: 0 382 808 1392
Size: 382 426 584
Fragments arranged by size:
584 426 382
PspOMI G'GGCC_C
Cuts at: 0 209 1392
Size: 209 1183
PstI C_TGCA'G
Cuts at: 0 208 1088 1205 1392
Size: 208 880 117 187
Fragments arranged by size:
880 208 187 117
PvuII CAG'CTG
Cuts at: 0 988 1392
Size: 988 404
RsrII CG'GwC_CG
Cuts at: 0 470 1392
Size: 470 922
SacI G_AGCT'C
Cuts at: 0 887 1392
Size: 887 505
SapI GCTCTTCn'nnn_
Cuts at: 0 297 1392
Size: 297 1095
SexAI A'CCwGG_T
Cuts at: 0 247 1392
Size: 247 1145
SfcI C'TryA_G
Cuts at: 0 204 1084 1201 1392
Size: 204 880 117 191
Fragments arranged by size:
880 204 191 117
SmlI C'TyrA_G
Cuts at: 0 215 1338 1392
Size: 215 1123 54
Fragments arranged by size:
1123 215 54
StuI AGG'CCT
Cuts at: 0 496 668 1392
Size: 496 172 724
Fragments arranged by size:
724 496 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 961 1056 1129 1392
Size: 961 95 73 263
Fragments arranged by size:
961 263 95 73
TatI w'GTAC_w
Cuts at: 0 200 713 1392
Size: 200 513 679
Fragments arranged by size:
679 513 200
Tth111I GACn'n_nGTC
Cuts at: 0 334 1392
Size: 334 1058
Enzymes that do cut:
AcuI AfeI AflIII AleI ApaI ApoI AvaI BanI
BanII BbsI BciVI BglI Bme1580I BpmI Bpu10I BpuEI
BsaI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BsrBI BsrDI BsrFI BstYI BtgI BtgZI CspCI
DraIII DrdI EaeI EagI EarI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FseI HaeII Hin4I MmeI MslI MspA1I
NaeI NgoMIV NspI PasI PciI PflMI PspOMI PstI
PvuII RsrII SacI SapI SexAI SfcI SmlI StuI
TaqII TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI
BsaWI BsiWI BsmBI BspEI BspHI BspMI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI
ClaI DraI EciI EcoRV FalI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MscI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI
PvuI SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StyI SwaI TstI XbaI XcmI XhoI XmaI XmnI
ZraI