Restriction map for
pF1KB5541
(Linear) (Six-base) MAPSORT of: KIBB5541.seq Check: 7165 from: 1 to: 2931
KIBB5541.orf
With 182 enzymes: SgfI *
September 4, 2008 14:04 ..
AatII G_ACGT'C
Cuts at: 0 647 1019 2408 2931
Size: 647 372 1389 523
Fragments arranged by size:
1389 647 523 372
AccI GT'mk_AC
Cuts at: 0 247 1114 2931
Size: 247 867 1817
Fragments arranged by size:
1817 867 247
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1214 2931
Size: 1214 1717
AflII C'TTAA_G
Cuts at: 0 1321 2931
Size: 1321 1610
AflIII A'CryG_T
Cuts at: 0 198 1535 2931
Size: 198 1337 1396
Fragments arranged by size:
1396 1337 198
AleI CACnn'nnGTG
Cuts at: 0 1753 2931
Size: 1753 1178
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 339 1372 2350 2931
Size: 339 1033 978 581
Fragments arranged by size:
1033 978 581 339
AlwNI CAG_nnn'CTG
Cuts at: 0 1464 1485 2931
Size: 1464 21 1446
Fragments arranged by size:
1464 1446 21
ApaI G_GGCC'C
Cuts at: 0 956 2931
Size: 956 1975
ApoI r'AATT_y
Cuts at: 0 1703 2662 2931
Size: 1703 959 269
Fragments arranged by size:
1703 959 269
AvaI C'yCGr_G
Cuts at: 0 49 509 1256 1297 1560 1807 2019 2931
Size: 49 460 747 41 263 247 212 912
Fragments arranged by size:
912 747 460 263 247 212 49 41
BamHI G'GATC_C
Cuts at: 0 653 1799 2445 2931
Size: 653 1146 646 486
Fragments arranged by size:
1146 653 646 486
BanI G'GyrC_C
Cuts at: 0 1564 2190 2362 2931
Size: 1564 626 172 569
Fragments arranged by size:
1564 626 569 172
BanII G_rGCy'C
Cuts at: 0 518 956 1529 1561 1994 2894 2903 2931
Size: 518 438 573 32 433 900 9 28
Fragments arranged by size:
900 573 518 438 433 32 28 9
BbsI GAAGACnn'nnnn_
Cuts at: 0 2240 2408 2931
Size: 2240 168 523
Fragments arranged by size:
2240 523 168
BbvCI CC'TCA_GC
Cuts at: 0 1742 2463 2931
Size: 1742 721 468
Fragments arranged by size:
1742 721 468
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 892 926 1915 1949 2457 2491 2931
Size: 892 34 989 34 508 34 440
Fragments arranged by size:
989 892 508 440 34 34 34
BclI T'GATC_A
Cuts at: 0 998 2931
Size: 998 1933
BfrBI ATG'CAT
Cuts at: 0 1404 2931
Size: 1404 1527
BglI GCCn_nnn'nGGC
Cuts at: 0 9 1633 2931
Size: 9 1624 1298
Fragments arranged by size:
1624 1298 9
BglII A'GATC_T
Cuts at: 0 2694 2931
Size: 2694 237
BlpI GC'TnA_GC
Cuts at: 0 2227 2931
Size: 2227 704
Bme1580I G_kGCm'C
Cuts at: 0 923 956 1567 1750 2931
Size: 923 33 611 183 1181
Fragments arranged by size:
1181 923 611 183 33
BmgBI CAC'GTC
Cuts at: 0 570 1538 1583 1785 2532 2801 2931
Size: 570 968 45 202 747 269 130
Fragments arranged by size:
968 747 570 269 202 130 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 2905 2931
Size: 2905 26
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2716 2931
Size: 2716 215
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1405 1468 1523 2008 2201 2500 2517
Size: 1147 258 63 55 485 193 299 17
Cuts at: 2517 2629 2903 2931
Size: 112 274 28
Fragments arranged by size:
1147 485 299 274 258 193 112 63
55 28 17
Bpu10I CC'TnA_GC
Cuts at: 0 1345 1396 1514 1742 2463 2931
Size: 1345 51 118 228 721 468
Fragments arranged by size:
1345 721 468 228 118 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1083 2931
Size: 1083 1848
BsaI GGTCTCn'nnnn_
Cuts at: 0 736 2054 2931
Size: 736 1318 877
Fragments arranged by size:
1318 877 736
BsaAI yAC'GTr
Cuts at: 0 2298 2416 2475 2931
Size: 2298 118 59 456
Fragments arranged by size:
2298 456 118 59
BsaBI GATnn'nnATC
Cuts at: 0 724 2931
Size: 724 2207
BsaHI Gr'CG_yC
Cuts at: 0 644 841 968 1016 2405 2924 2931
Size: 644 197 127 48 1389 519 7
Fragments arranged by size:
1389 644 519 197 127 48 7
BsaWI w'CCGG_w
Cuts at: 0 394 518 2931
Size: 394 124 2413
Fragments arranged by size:
2413 394 124
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 652 682 2023 2053 2931
Size: 652 30 1341 30 878
Fragments arranged by size:
1341 878 652 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1282 2689 2809 2931
Size: 1282 1407 120 122
Fragments arranged by size:
1407 1282 122 120
BseYI C'CCAG_C
Cuts at: 0 1006 1367 1576 2763 2903 2931
Size: 1006 361 209 1187 140 28
Fragments arranged by size:
1187 1006 361 209 140 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1872 1955 2871 2931
Size: 331 1541 83 916 60
Fragments arranged by size:
1541 916 331 83 60
BsiEI CG_ry'CG
Cuts at: 0 2573 2931
Size: 2573 358
BsiHKAI G_wGCw'C
Cuts at: 0 518 1351 1529 1561 1865 2480 2931
Size: 518 833 178 32 304 615 451
Fragments arranged by size:
833 615 518 451 304 178 32
BsmI GAATG_Cn'
Cuts at: 0 531 2931
Size: 531 2400
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2752 2807 2931
Size: 2752 55 124
Fragments arranged by size:
2752 124 55
Bsp1286I G_dGCh'C
Cuts at: 0 518 923 956 1351 1529 1561 1567 1750
Size: 518 405 33 395 178 32 6 183
Cuts at: 1750 1865 1994 2480 2894 2903 2931
Size: 115 129 486 414 9 28
Fragments arranged by size:
518 486 414 405 395 183 178 129
115 33 32 28 9 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 177 2663 2931
Size: 177 2486 268
Fragments arranged by size:
2486 268 177
BsrDI GCAATG_nn'
Cuts at: 0 1916 2408 2931
Size: 1916 492 523
Fragments arranged by size:
1916 523 492
BsrFI r'CCGG_y
Cuts at: 0 3 1901 2931
Size: 3 1898 1030
Fragments arranged by size:
1898 1030 3
BssSI C'ACGA_G
Cuts at: 0 901 2857 2887 2931
Size: 901 1956 30 44
Fragments arranged by size:
1956 901 44 30
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1485 2931
Size: 1485 1446
BstXI CCAn_nnnn'nTGG
Cuts at: 0 964 1125 1501 2323 2931
Size: 964 161 376 822 608
Fragments arranged by size:
964 822 608 376 161
BstYI r'GATC_y
Cuts at: 0 174 225 653 1454 1799 2445 2694 2931
Size: 174 51 428 801 345 646 249 237
Fragments arranged by size:
801 646 428 345 249 237 174 51
BstZ17I GTA'TAC
Cuts at: 0 248 2931
Size: 248 2683
Bsu36I CC'TnA_GG
Cuts at: 0 560 2931
Size: 560 2371
BtgI C'CryG_G
Cuts at: 0 924 1307 1734 2603 2840 2931
Size: 924 383 427 869 237 91
Fragments arranged by size:
924 869 427 383 237 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2455 2931
Size: 2455 476
BtsI GCAGTG_nn'
Cuts at: 0 553 2162 2931
Size: 553 1609 769
Fragments arranged by size:
1609 769 553
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 182 217 519 554 2931
Size: 182 35 302 35 2377
Fragments arranged by size:
2377 302 182 35 35
DraI TTT'AAA
Cuts at: 0 167 2931
Size: 167 2764
DraIII CAC_nnn'GTG
Cuts at: 0 594 794 2931
Size: 594 200 2137
Fragments arranged by size:
2137 594 200
DrdI GACnn_nn'nnGTC
Cuts at: 0 765 1834 2766 2931
Size: 765 1069 932 165
Fragments arranged by size:
1069 932 765 165
EaeI y'GGCC_r
Cuts at: 0 1787 2931
Size: 1787 1144
EarI CTCTTCn'nnn_
Cuts at: 0 200 461 1789 2872 2931
Size: 200 261 1328 1083 59
Fragments arranged by size:
1328 1083 261 200 59
EcoICRI GAG'CTC
Cuts at: 0 516 1527 1559 2931
Size: 516 1011 32 1372
Fragments arranged by size:
1372 1011 516 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1214 1405 1468 1523 2008 2201 2500
Size: 1147 67 191 63 55 485 193 299
Cuts at: 2500 2517 2629 2903 2931
Size: 17 112 274 28
Fragments arranged by size:
1147 485 299 274 193 191 112 67
63 55 28 17
EcoO109I rG'GnC_Cy
Cuts at: 0 189 952 953 1302 1592 2669 2768 2931
Size: 189 763 1 349 290 1077 99 163
Fragments arranged by size:
1077 763 349 290 189 163 99 1
FspI TGC'GCA
Cuts at: 0 1976 2931
Size: 1976 955
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 831 863 1389 1421 2433 2465
Size: 619 32 180 32 526 32 1012 32
Cuts at: 2465 2931
Size: 466
Fragments arranged by size:
1012 619 526 466 180 32 32 32
32
HincII GTy'rAC
Cuts at: 0 107 1221 2931
Size: 107 1114 1710
Fragments arranged by size:
1710 1114 107
MfeI C'AATT_G
Cuts at: 0 1751 2931
Size: 1751 1180
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1622 2153 2931
Size: 1622 531 778
Fragments arranged by size:
1622 778 531
MslI CAynn'nnrTG
Cuts at: 0 321 490 728 1753 2089 2931
Size: 321 169 238 1025 336 842
Fragments arranged by size:
1025 842 336 321 238 169
MspA1I CmG'CkG
Cuts at: 0 699 926 1485 1842 2340 2931
Size: 699 227 559 357 498 591
Fragments arranged by size:
699 591 559 498 357 227
NaeI GCC'GGC
Cuts at: 0 5 2931
Size: 5 2926
NcoI C'CATG_G
Cuts at: 0 1734 2840 2931
Size: 1734 1106 91
Fragments arranged by size:
1734 1106 91
NgoMIV G'CCGG_C
Cuts at: 0 3 2931
Size: 3 2928
NsiI A_TGCA'T
Cuts at: 0 1406 2931
Size: 1406 1525
NspI r_CATG'y
Cuts at: 0 130 202 1404 1770 2931
Size: 130 72 1202 366 1161
Fragments arranged by size:
1202 1161 366 130 72
PasI CC'CwG_GG
Cuts at: 0 1589 2931
Size: 1589 1342
PciI A'CATG_T
Cuts at: 0 198 2931
Size: 198 2733
PfoI T'CCnGG_A
Cuts at: 0 1029 1529 2525 2931
Size: 1029 500 996 406
Fragments arranged by size:
1029 996 500 406
PmlI CAC'GTG
Cuts at: 0 2416 2475 2931
Size: 2416 59 456
Fragments arranged by size:
2416 456 59
PpuMI rG'GwC_Cy
Cuts at: 0 189 2669 2768 2931
Size: 189 2480 99 163
Fragments arranged by size:
2480 189 163 99
PspOMI G'GGCC_C
Cuts at: 0 952 2931
Size: 952 1979
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2239 2271 2931
Size: 2239 32 660
Fragments arranged by size:
2239 660 32
PstI C_TGCA'G
Cuts at: 0 188 1469 2009 2280 2339 2371 2748 2931
Size: 188 1281 540 271 59 32 377 183
Fragments arranged by size:
1281 540 377 271 188 183 59 32
PvuI CG_AT'CG
Cuts at: 0 2573 2931
Size: 2573 358
PvuII CAG'CTG
Cuts at: 0 1485 1842 2931
Size: 1485 357 1089
Fragments arranged by size:
1485 1089 357
SacI G_AGCT'C
Cuts at: 0 518 1529 1561 2931
Size: 518 1011 32 1370
Fragments arranged by size:
1370 1011 518 32
SacII CC_GC'GG
Cuts at: 0 927 2931
Size: 927 2004
SanDI GG'GwC_CC
Cuts at: 0 2768 2931
Size: 2768 163
SapI GCTCTTCn'nnn_
Cuts at: 0 200 2931
Size: 200 2731
SbfI CC_TGCA'GG
Cuts at: 0 188 2931
Size: 188 2743
SexAI A'CCwGG_T
Cuts at: 0 1214 2931
Size: 1214 1717
SfcI C'TryA_G
Cuts at: 0 184 1465 2005 2276 2335 2367 2744 2931
Size: 184 1281 540 271 59 32 377 187
Fragments arranged by size:
1281 540 377 271 187 184 59 32
SmlI C'TyrA_G
Cuts at: 0 1062 1321 2931
Size: 1062 259 1610
Fragments arranged by size:
1610 1062 259
SnaBI TAC'GTA
Cuts at: 0 2298 2931
Size: 2298 633
SphI G_CATG'C
Cuts at: 0 130 1404 2931
Size: 130 1274 1527
Fragments arranged by size:
1527 1274 130
StuI AGG'CCT
Cuts at: 0 275 2931
Size: 275 2656
StyI C'CwwG_G
Cuts at: 0 812 1631 1718 1734 2840 2931
Size: 812 819 87 16 1106 91
Fragments arranged by size:
1106 819 812 91 87 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1359 1511 2350 2402 2931
Size: 1359 152 839 52 529
Fragments arranged by size:
1359 839 529 152 52
TatI w'GTAC_w
Cuts at: 0 1913 2931
Size: 1913 1018
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 580 612 634 666 2023 2055 2063 2095
Size: 580 32 22 32 1357 32 8 32
Cuts at: 2095 2931
Size: 836
Fragments arranged by size:
1357 836 580 32 32 32 32 22
8
Tth111I GACn'n_nGTC
Cuts at: 0 414 2931
Size: 414 2517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 303 1725 2022 2355 2931
Size: 303 1422 297 333 576
Fragments arranged by size:
1422 576 333 303 297
ZraI GAC'GTC
Cuts at: 0 645 1017 2406 2931
Size: 645 372 1389 525
Fragments arranged by size:
1389 645 525 372
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AleI AlfI AlwNI
ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrDI BsrFI BssSI BstAPI
BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
FspI Hin4I HincII MfeI MmeI MslI MspA1I NaeI
NcoI NgoMIV NsiI NspI PasI PciI PfoI PmlI
PpuMI PspOMI PsrI PstI PvuI PvuII SacI SacII
SanDI SapI SbfI SexAI SfcI SmlI SnaBI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AloI ApaLI
AscI AseI AsiSI AvrII BaeI BbeI BciVI BmtI
BsiWI BspEI BspHI BsrBI BsrGI BssHII BstBI BstEII
ClaI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspAI HaeII HindIII HpaI KasI KpnI MluI MscI
NarI NdeI NheI NotI NruI PacI PflMI PmeI
PpiI PshAI PsiI PspXI RsrII SalI ScaI SfiI
SfoI SgfI SgrAI SmaI SpeI SrfI SspI SwaI
XbaI XhoI XmaI XmnI