Restriction map for
pF1KB5535
(Linear) (Six-base) MAPSORT of: KIBB5535.seq Check: 9634 from: 1 to: 1389
KIBB5535.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 87 1389
Size: 87 1302
AccI GT'mk_AC
Cuts at: 0 816 1389
Size: 816 573
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 1389
Size: 187 1202
AflII C'TTAA_G
Cuts at: 0 466 1389
Size: 466 923
AlwNI CAG_nnn'CTG
Cuts at: 0 210 979 1252 1389
Size: 210 769 273 137
Fragments arranged by size:
769 273 210 137
ApaI G_GGCC'C
Cuts at: 0 14 1106 1389
Size: 14 1092 283
Fragments arranged by size:
1092 283 14
ApoI r'AATT_y
Cuts at: 0 471 1389
Size: 471 918
BamHI G'GATC_C
Cuts at: 0 1319 1389
Size: 1319 70
BanI G'GyrC_C
Cuts at: 0 58 109 1288 1389
Size: 58 51 1179 101
Fragments arranged by size:
1179 101 58 51
BanII G_rGCy'C
Cuts at: 0 14 133 1106 1389
Size: 14 119 973 283
Fragments arranged by size:
973 283 119 14
BbeI G_GCGC'C
Cuts at: 0 62 1389
Size: 62 1327
BbsI GAAGACnn'nnnn_
Cuts at: 0 111 1010 1389
Size: 111 899 379
Fragments arranged by size:
899 379 111
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 61 95 1389
Size: 61 34 1294
Fragments arranged by size:
1294 61 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 207 1389
Size: 207 1182
BclI T'GATC_A
Cuts at: 0 510 1389
Size: 510 879
Bme1580I G_kGCm'C
Cuts at: 0 14 112 1106 1291 1389
Size: 14 98 994 185 98
Fragments arranged by size:
994 185 98 98 14
BmrI ACTGGGnnnn_n'
Cuts at: 0 377 408 1389
Size: 377 31 981
Fragments arranged by size:
981 377 31
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 729 1389
Size: 117 612 660
Fragments arranged by size:
660 612 117
Bpu10I CC'TnA_GC
Cuts at: 0 847 1389
Size: 847 542
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 1105 1389
Size: 869 236 284
Fragments arranged by size:
869 284 236
BsaI GGTCTCn'nnnn_
Cuts at: 0 1175 1389
Size: 1175 214
BsaHI Gr'CG_yC
Cuts at: 0 59 1389
Size: 59 1330
BsaWI w'CCGG_w
Cuts at: 0 412 1316 1389
Size: 412 904 73
Fragments arranged by size:
904 412 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 600 630 850 880 969 999 1389
Size: 600 30 220 30 89 30 390
Fragments arranged by size:
600 390 220 89 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1014 1389
Size: 1014 375
BseYI C'CCAG_C
Cuts at: 0 7 105 143 1283 1295 1389
Size: 7 98 38 1140 12 94
Fragments arranged by size:
1140 98 94 38 12 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 635 1389
Size: 72 563 754
Fragments arranged by size:
754 563 72
Bsp1286I G_dGCh'C
Cuts at: 0 14 112 133 1106 1291 1389
Size: 14 98 21 973 185 98
Fragments arranged by size:
973 185 98 98 21 14
BspEI T'CCGG_A
Cuts at: 0 1316 1389
Size: 1316 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 87 110 657 1389
Size: 87 23 547 732
Fragments arranged by size:
732 547 87 23
BsrDI GCAATG_nn'
Cuts at: 0 1071 1389
Size: 1071 318
BsrFI r'CCGG_y
Cuts at: 0 1291 1389
Size: 1291 98
BstBI TT'CG_AA
Cuts at: 0 747 1389
Size: 747 642
BstEII G'GTnAC_C
Cuts at: 0 339 1389
Size: 339 1050
BstXI CCAn_nnnn'nTGG
Cuts at: 0 701 940 1171 1389
Size: 701 239 231 218
Fragments arranged by size:
701 239 231 218
BstYI r'GATC_y
Cuts at: 0 1157 1319 1389
Size: 1157 162 70
Fragments arranged by size:
1157 162 70
BstZ17I GTA'TAC
Cuts at: 0 817 1389
Size: 817 572
Bsu36I CC'TnA_GG
Cuts at: 0 854 1389
Size: 854 535
BtgI C'CryG_G
Cuts at: 0 1106 1389
Size: 1106 283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 164 1389
Size: 164 1225
BtsI GCAGTG_nn'
Cuts at: 0 1367 1389
Size: 1367 22
EaeI y'GGCC_r
Cuts at: 0 243 618 1389
Size: 243 375 771
Fragments arranged by size:
771 375 243
EarI CTCTTCn'nnn_
Cuts at: 0 320 1389
Size: 320 1069
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 117 187 729 1389
Size: 117 70 542 660
Fragments arranged by size:
660 542 117 70
EcoO109I rG'GnC_Cy
Cuts at: 0 11 1389
Size: 11 1378
EcoRI G'AATT_C
Cuts at: 0 471 1389
Size: 471 918
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1202 1389
Size: 1202 187
HaeII r_GCGC'y
Cuts at: 0 62 1389
Size: 62 1327
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 850 882 1234 1266 1389
Size: 850 32 352 32 123
Fragments arranged by size:
850 352 123 32 32
HincII GTy'rAC
Cuts at: 0 156 453 1389
Size: 156 297 936
Fragments arranged by size:
936 297 156
HindIII A'AGCT_T
Cuts at: 0 956 1081 1389
Size: 956 125 308
Fragments arranged by size:
956 308 125
KasI G'GCGC_C
Cuts at: 0 58 1389
Size: 58 1331
MfeI C'AATT_G
Cuts at: 0 1346 1389
Size: 1346 43
MscI TGG'CCA
Cuts at: 0 245 620 1389
Size: 245 375 769
Fragments arranged by size:
769 375 245
MslI CAynn'nnrTG
Cuts at: 0 1360 1389
Size: 1360 29
MspA1I CmG'CkG
Cuts at: 0 137 770 1283 1389
Size: 137 633 513 106
Fragments arranged by size:
633 513 137 106
NarI GG'CG_CC
Cuts at: 0 59 1389
Size: 59 1330
NcoI C'CATG_G
Cuts at: 0 1106 1389
Size: 1106 283
NdeI CA'TA_TG
Cuts at: 0 222 1389
Size: 222 1167
PasI CC'CwG_GG
Cuts at: 0 591 1389
Size: 591 798
PspOMI G'GGCC_C
Cuts at: 0 10 1102 1389
Size: 10 1092 287
Fragments arranged by size:
1092 287 10
PvuII CAG'CTG
Cuts at: 0 770 1283 1389
Size: 770 513 106
Fragments arranged by size:
770 513 106
SfoI GGC'GCC
Cuts at: 0 60 1389
Size: 60 1329
SmlI C'TyrA_G
Cuts at: 0 466 884 1084 1389
Size: 466 418 200 305
Fragments arranged by size:
466 418 305 200
StyI C'CwwG_G
Cuts at: 0 231 267 637 1106 1389
Size: 231 36 370 469 283
Fragments arranged by size:
469 370 283 231 36
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 359 363 1389
Size: 359 4 1026
Fragments arranged by size:
1026 359 4
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 407 439 600 632 969 1001 1362 1389
Size: 407 32 161 32 337 32 361 27
Fragments arranged by size:
407 361 337 161 32 32 32 27
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 760 1389
Size: 760 629
Enzymes that do cut:
AarI AccI AcuI AflII AlwNI ApaI ApoI BamHI
BanI BanII BbeI BbsI BcgI BciVI BclI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrDI BsrFI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI EaeI EarI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII KasI MfeI MscI MslI MspA1I
NarI NcoI NdeI PasI PspOMI PvuII SfoI SmlI
StyI TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbvCI BfrBI BglI BglII BlpI BmgBI BmtI
BplI BsaAI BsaBI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspHI BsrBI BsrGI BssHII BssSI BstAPI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRV
FseI FspI FspAI HpaI KpnI MluI MmeI NaeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI Tth111I XbaI XhoI XmaI XmnI ZraI