(Linear) (Six-base) MAPSORT of: KIBB5399.seq  Check: 2734  from: 1  to: 573



KIBB5399.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AccI GT'mk_AC

Cuts at:      0     452     573 
   Size:        452     121
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     573 
   Size:         50     523
 
AleI CACnn'nnGTG

Cuts at:      0     352     573 
   Size:        352     221
 
AlwNI CAG_nnn'CTG

Cuts at:      0     300     573 
   Size:        300     273
 
ApoI r'AATT_y

Cuts at:      0     139     508     573 
   Size:        139     369      65

  Fragments arranged by size: 

                 369     139      65
 
AvaI C'yCGr_G

Cuts at:      0     133     350     573 
   Size:        133     217     223

  Fragments arranged by size: 

                 223     217     133
 
BanI G'GyrC_C

Cuts at:      0     232     573 
   Size:        232     341
 
BclI T'GATC_A

Cuts at:      0     404     573 
   Size:        404     169
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     315     573 
   Size:        315     258
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     261     385     573 
   Size:        261     124     188

  Fragments arranged by size: 

                 261     188     124
 
Bpu10I CC'TnA_GC

Cuts at:      0     440     573 
   Size:        440     133
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      64      88     573 
   Size:         64      24     485

  Fragments arranged by size: 

                 485      64      24
 
BsaHI Gr'CG_yC

Cuts at:      0     179     436     573 
   Size:        179     257     137

  Fragments arranged by size: 

                 257     179     137
 
BseYI C'CCAG_C

Cuts at:      0     194     573 
   Size:        194     379
 
BsiHKAI G_wGCw'C

Cuts at:      0      58     573 
   Size:         58     515
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     170     573 
   Size:        170     403
 
Bsp1286I G_dGCh'C

Cuts at:      0      58     573 
   Size:         58     515
 
BspHI T'CATG_A

Cuts at:      0     407     573 
   Size:        407     166
 
BtsI GCAGTG_nn'

Cuts at:      0      91     562     573 
   Size:         91     471      11

  Fragments arranged by size: 

                 471      91      11
 
EarI CTCTTCn'nnn_

Cuts at:      0      46     573 
   Size:         46     527
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      50     261     385     573 
   Size:         50     211     124     188

  Fragments arranged by size: 

                 211     188     124      50
 
EcoO109I rG'GnC_Cy

Cuts at:      0      42     573 
   Size:         42     531
 
EcoRI G'AATT_C

Cuts at:      0     508     573 
   Size:        508      65
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     114     146     239     271     573 
   Size:        114      32      93      32     302

  Fragments arranged by size: 

                 302     114      93      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     352     573 
   Size:        352     221
 
NspI r_CATG'y

Cuts at:      0     202     573 
   Size:        202     371
 
PpuMI rG'GwC_Cy

Cuts at:      0      42     573 
   Size:         42     531
 
PstI C_TGCA'G

Cuts at:      0     557     573 
   Size:        557      16
 
SapI GCTCTTCn'nnn_

Cuts at:      0      46     573 
   Size:         46     527
 
SexAI A'CCwGG_T

Cuts at:      0      44     573 
   Size:         44     529
 
SfcI C'TryA_G

Cuts at:      0     553     573 
   Size:        553      20
 
SmlI C'TyrA_G

Cuts at:      0      79     103     133     573 
   Size:         79      24      30     440

  Fragments arranged by size: 

                 440      79      30      24
 
SphI G_CATG'C

Cuts at:      0     202     573 
   Size:        202     371
 
XhoI C'TCGA_G

Cuts at:      0     133     573 
   Size:        133     440
 

 Enzymes that do cut:

     AccI     AcuI     AleI    AlwNI     ApoI     AvaI     BanI     BclI
     BmrI     BpmI   Bpu10I    BpuEI    BsaHI    BseYI  BsiHKAI    BsmBI
 Bsp1286I    BspHI     BtsI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I
     MslI     NspI    PpuMI     PstI     SapI    SexAI     SfcI     SmlI
     SphI     XhoI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmtI
     BplI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI    BsiWI     BsmI    BspEI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI
     XmnI     ZraI