(Linear) (Six-base) MAPSORT of: KIBB5375.seq  Check: 6632  from: 1  to: 675



KIBB5375.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     302     400     574     675 
   Size:        302      98     174     101

  Fragments arranged by size: 

                 302     174     101      98
 
AflIII A'CryG_T

Cuts at:      0     110     266     675 
   Size:        110     156     409

  Fragments arranged by size: 

                 409     156     110
 
AleI CACnn'nnGTG

Cuts at:      0     475     675 
   Size:        475     200
 
ApoI r'AATT_y

Cuts at:      0     160     442     675 
   Size:        160     282     233

  Fragments arranged by size: 

                 282     233     160
 
AvaI C'yCGr_G

Cuts at:      0      25     675 
   Size:         25     650
 
BciVI GTATCCnnnnn_n'

Cuts at:      0      78     675 
   Size:         78     597
 
BclI T'GATC_A

Cuts at:      0     612     675 
   Size:        612      63
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      42     645     675 
   Size:         42     603      30

  Fragments arranged by size: 

                 603      42      30
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     675 
   Size:         94     581
 
BsaAI yAC'GTr

Cuts at:      0     113     675 
   Size:        113     562
 
BsaBI GATnn'nnATC

Cuts at:      0     631     675 
   Size:        631      44
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      91     121     675 
   Size:         91      30     554

  Fragments arranged by size: 

                 554      91      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     112     675 
   Size:        112     563
 
BsrDI GCAATG_nn'

Cuts at:      0     237     675 
   Size:        237     438
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     435     534     675 
   Size:        435      99     141

  Fragments arranged by size: 

                 435     141      99
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     234     675 
   Size:        234     441
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     252     287     675 
   Size:        252      35     388

  Fragments arranged by size: 

                 388     252      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     392     675 
   Size:        392     283
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      52     675 
   Size:         52     623
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      94     302     400     574     675 
   Size:         94     208      98     174     101

  Fragments arranged by size: 

                 208     174     101      98      94
 
EcoRV GAT'ATC

Cuts at:      0     498     675 
   Size:        498     177
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     489     675 
   Size:        489     186
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     290     322     529     561     675 
   Size:        290      32     207      32     114

  Fragments arranged by size: 

                 290     207     114      32      32
 
MslI CAynn'nnrTG

Cuts at:      0     475     675 
   Size:        475     200
 
NspI r_CATG'y

Cuts at:      0     270     675 
   Size:        270     405
 
PciI A'CATG_T

Cuts at:      0     266     675 
   Size:        266     409
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     150     324     675 
   Size:        150     174     351

  Fragments arranged by size: 

                 351     174     150
 
PfoI T'CCnGG_A

Cuts at:      0      71     200     675 
   Size:         71     129     475

  Fragments arranged by size: 

                 475     129      71
 
PmlI CAC'GTG

Cuts at:      0     113     675 
   Size:        113     562
 
SapI GCTCTTCn'nnn_

Cuts at:      0     392     675 
   Size:        392     283
 
ScaI AGT'ACT

Cuts at:      0     391     675 
   Size:        391     284
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     238     675 
   Size:        238     437
 
TatI w'GTAC_w

Cuts at:      0     389     675 
   Size:        389     286
 
XmnI GAAnn'nnTTC

Cuts at:      0     293     421     559     675 
   Size:        293     128     138     116

  Fragments arranged by size: 

                 293     138     128     116
 

 Enzymes that do cut:

     AcuI   AflIII     AleI     ApoI     AvaI    BciVI     BclI     BmrI
     BpmI    BsaAI    BsaBI    BsaXI    BseRI    BsrDI    BstXI    BtgZI
    CspCI     EarI     EciI  Eco57MI    EcoRV     FalI    Hin4I     MslI
     NspI     PciI    PflMI     PfoI     PmlI     SapI     ScaI    TaqII
     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BseYI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtgI     BtsI     ClaI
     DraI   DraIII     DrdI     EaeI     EagI  EcoICRI    EcoNI EcoO109I
    EcoRI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SbfI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     ZraI