(Linear) (Six-base) MAPSORT of: KIBB5364.seq  Check: 9551  from: 1  to: 1185



KIBB5364.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     273    1185 
   Size:        273     912
 
AccI GT'mk_AC

Cuts at:      0    1106    1185 
   Size:       1106      79
 
AclI AA'CG_TT

Cuts at:      0     675    1185 
   Size:        675     510
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     259    1185 
   Size:         98     161     926

  Fragments arranged by size: 

                 926     161      98
 
ApoI r'AATT_y

Cuts at:      0     231     561     801    1185 
   Size:        231     330     240     384

  Fragments arranged by size: 

                 384     330     240     231
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     512     545    1185 
   Size:        512      33     640

  Fragments arranged by size: 

                 640     512      33
 
BanI G'GyrC_C

Cuts at:      0     207    1185 
   Size:        207     978
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     452     585    1185 
   Size:        452     133     600

  Fragments arranged by size: 

                 600     452     133
 
BglI GCCn_nnn'nGGC

Cuts at:      0     314    1185 
   Size:        314     871
 
BlpI GC'TnA_GC

Cuts at:      0     926    1185 
   Size:        926     259
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1062    1185 
   Size:       1062     123
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      93     123    1185 
   Size:         93      30    1062

  Fragments arranged by size: 

                1062      93      30
 
BseYI C'CCAG_C

Cuts at:      0     831    1185 
   Size:        831     354
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     155    1185 
   Size:        155    1030
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     273    1160    1185 
   Size:        273     887      25

  Fragments arranged by size: 

                 887     273      25
 
BssSI C'ACGA_G

Cuts at:      0     884    1185 
   Size:        884     301
 
BstBI TT'CG_AA

Cuts at:      0    1142    1185 
   Size:       1142      43
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     825    1185 
   Size:        825     360
 
BstZ17I GTA'TAC

Cuts at:      0    1107    1185 
   Size:       1107      78
 
BtgI C'CryG_G

Cuts at:      0      76     142    1185 
   Size:         76      66    1043

  Fragments arranged by size: 

                1043      76      66
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     656    1185 
   Size:        656     529
 
EarI CTCTTCn'nnn_

Cuts at:      0     332    1185 
   Size:        332     853
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     259    1185 
   Size:         98     161     926

  Fragments arranged by size: 

                 926     161      98
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     113    1175    1185 
   Size:        113    1062      10

  Fragments arranged by size: 

                1062     113      10
 
EcoRI G'AATT_C

Cuts at:      0     231    1185 
   Size:        231     954
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1057    1089    1185 
   Size:       1057      32      96

  Fragments arranged by size: 

                1057      96      32
 
HincII GTy'rAC

Cuts at:      0     632    1076    1185 
   Size:        632     444     109

  Fragments arranged by size: 

                 632     444     109
 
HindIII A'AGCT_T

Cuts at:      0     606    1185 
   Size:        606     579
 
HpaI GTT'AAC

Cuts at:      0     632    1185 
   Size:        632     553
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     949    1185 
   Size:        949     236
 
MspA1I CmG'CkG

Cuts at:      0      50     361    1185 
   Size:         50     311     824

  Fragments arranged by size: 

                 824     311      50
 
NcoI C'CATG_G

Cuts at:      0     142    1185 
   Size:        142    1043
 
NspI r_CATG'y

Cuts at:      0     630    1185 
   Size:        630     555
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     122     154    1185 
   Size:        122      32    1031

  Fragments arranged by size: 

                1031     122      32
 
PstI C_TGCA'G

Cuts at:      0      94    1185 
   Size:         94    1091
 
PvuII CAG'CTG

Cuts at:      0      50    1185 
   Size:         50    1135
 
SfcI C'TryA_G

Cuts at:      0      90    1185 
   Size:         90    1095
 
SspI AAT'ATT

Cuts at:      0     402    1185 
   Size:        402     783
 
StyI C'CwwG_G

Cuts at:      0     142    1185 
   Size:        142    1043
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     491    1185 
   Size:        491     694
 
TatI w'GTAC_w

Cuts at:      0     427    1185 
   Size:        427     758
 
XbaI T'CTAG_A

Cuts at:      0     708    1185 
   Size:        708     477
 
XmnI GAAnn'nnTTC

Cuts at:      0     716    1185 
   Size:        716     469
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     ApoI     BaeI     BanI     BbsI
     BglI     BlpI     BplI    BsaXI    BseYI     BsgI    BspMI    BssSI
    BstBI    BstXI  BstZ17I     BtgI     DrdI     EarI  Eco57MI    EcoNI
    EcoRI    Hin4I   HincII  HindIII     HpaI     MmeI   MspA1I     NcoI
     NspI     PpiI     PstI    PvuII     SfcI     SspI     StyI    TaqII
     TatI     XbaI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII    BamHI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI    BglII Bme1580I    BmgBI     BmrI     BmtI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII   BstAPI   BstEII    BstYI
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EaeI
     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII     KasI     KpnI     MfeI     MluI     MscI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     StuI     SwaI     TstI  Tth111I     XcmI     XhoI     XmaI
     ZraI