Restriction map for
pF1KB5349
(Linear) (Six-base) MAPSORT of: KIBB5349.seq Check: 9715 from: 1 to: 1128
KIBB5349.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 100 772 1128
Size: 100 672 356
Fragments arranged by size:
672 356 100
AflIII A'CryG_T
Cuts at: 0 506 1128
Size: 506 622
AleI CACnn'nnGTG
Cuts at: 0 1075 1128
Size: 1075 53
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1108 1128
Size: 1108 20
AlwNI CAG_nnn'CTG
Cuts at: 0 571 696 750 804 1128
Size: 571 125 54 54 324
Fragments arranged by size:
571 324 125 54 54
ApaI G_GGCC'C
Cuts at: 0 47 1128
Size: 47 1081
ApaLI G'TGCA_C
Cuts at: 0 1068 1128
Size: 1068 60
ApoI r'AATT_y
Cuts at: 0 196 812 1128
Size: 196 616 316
Fragments arranged by size:
616 316 196
AvaI C'yCGr_G
Cuts at: 0 46 1013 1128
Size: 46 967 115
Fragments arranged by size:
967 115 46
BamHI G'GATC_C
Cuts at: 0 138 1128
Size: 138 990
BanI G'GyrC_C
Cuts at: 0 65 1128
Size: 65 1063
BanII G_rGCy'C
Cuts at: 0 40 47 83 360 1128
Size: 40 7 36 277 768
Fragments arranged by size:
768 277 40 36 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 760 1128
Size: 760 368
Bme1580I G_kGCm'C
Cuts at: 0 47 1072 1128
Size: 47 1025 56
Fragments arranged by size:
1025 56 47
BmtI G_CTAG'C
Cuts at: 0 607 1128
Size: 607 521
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 181 664 1128
Size: 181 483 464
Fragments arranged by size:
483 464 181
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 459 685 1078 1128
Size: 94 365 226 393 50
Fragments arranged by size:
393 365 226 94 50
BsaI GGTCTCn'nnnn_
Cuts at: 0 169 1128
Size: 169 959
BsaAI yAC'GTr
Cuts at: 0 246 1128
Size: 246 882
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1051 1081 1128
Size: 1051 30 47
Fragments arranged by size:
1051 47 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 91 637 1128
Size: 91 546 491
Fragments arranged by size:
546 491 91
BseYI C'CCAG_C
Cuts at: 0 597 797 1128
Size: 597 200 331
Fragments arranged by size:
597 331 200
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1027 1128
Size: 154 873 101
Fragments arranged by size:
873 154 101
BsiHKAI G_wGCw'C
Cuts at: 0 40 83 360 1072 1106 1128
Size: 40 43 277 712 34 22
Fragments arranged by size:
712 277 43 40 34 22
Bsp1286I G_dGCh'C
Cuts at: 0 40 47 83 360 1072 1106 1128
Size: 40 7 36 277 712 34 22
Fragments arranged by size:
712 277 40 36 34 22 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 861 1128
Size: 861 267
BsrBI CCG'CTC
Cuts at: 0 738 1128
Size: 738 390
BsrFI r'CCGG_y
Cuts at: 0 686 1128
Size: 686 442
BssSI C'ACGA_G
Cuts at: 0 226 299 1128
Size: 226 73 829
Fragments arranged by size:
829 226 73
BstAPI GCAn_nnn'nTGC
Cuts at: 0 660 1128
Size: 660 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 160 1128
Size: 160 968
BstYI r'GATC_y
Cuts at: 0 138 558 1128
Size: 138 420 570
Fragments arranged by size:
570 420 138
Bsu36I CC'TnA_GG
Cuts at: 0 673 1128
Size: 673 455
BtsI GCAGTG_nn'
Cuts at: 0 335 697 1128
Size: 335 362 431
Fragments arranged by size:
431 362 335
DraIII CAC_nnn'GTG
Cuts at: 0 210 660 1128
Size: 210 450 468
Fragments arranged by size:
468 450 210
DrdI GACnn_nn'nnGTC
Cuts at: 0 988 1128
Size: 988 140
EarI CTCTTCn'nnn_
Cuts at: 0 1075 1128
Size: 1075 53
EcoICRI GAG'CTC
Cuts at: 0 38 81 358 1128
Size: 38 43 277 770
Fragments arranged by size:
770 277 43 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 94 100 459 685 772 1078 1128
Size: 94 6 359 226 87 306 50
Fragments arranged by size:
359 306 226 94 87 50 6
EcoO109I rG'GnC_Cy
Cuts at: 0 378 939 1128
Size: 378 561 189
Fragments arranged by size:
561 378 189
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 817 1128
Size: 817 311
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 249 281 1128
Size: 249 32 847
Fragments arranged by size:
847 249 32
HincII GTy'rAC
Cuts at: 0 549 1128
Size: 549 579
HindIII A'AGCT_T
Cuts at: 0 172 1128
Size: 172 956
MfeI C'AATT_G
Cuts at: 0 350 401 1128
Size: 350 51 727
Fragments arranged by size:
727 350 51
MluI A'CGCG_T
Cuts at: 0 506 1128
Size: 506 622
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1001 1128
Size: 1001 127
MslI CAynn'nnrTG
Cuts at: 0 835 1075 1090 1128
Size: 835 240 15 38
Fragments arranged by size:
835 240 38 15
MspA1I CmG'CkG
Cuts at: 0 58 108 1128
Size: 58 50 1020
Fragments arranged by size:
1020 58 50
NheI G'CTAG_C
Cuts at: 0 603 1128
Size: 603 525
PpuMI rG'GwC_Cy
Cuts at: 0 378 1128
Size: 378 750
PspOMI G'GGCC_C
Cuts at: 0 43 1128
Size: 43 1085
PvuII CAG'CTG
Cuts at: 0 108 1128
Size: 108 1020
SacI G_AGCT'C
Cuts at: 0 40 83 360 1128
Size: 40 43 277 768
Fragments arranged by size:
768 277 43 40
SapI GCTCTTCn'nnn_
Cuts at: 0 1075 1128
Size: 1075 53
SfcI C'TryA_G
Cuts at: 0 22 382 1128
Size: 22 360 746
Fragments arranged by size:
746 360 22
SmaI CCC'GGG
Cuts at: 0 48 1128
Size: 48 1080
SrfI GCCC'GGGC
Cuts at: 0 48 1128
Size: 48 1080
StyI C'CwwG_G
Cuts at: 0 153 1128
Size: 153 975
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 993 1128
Size: 993 135
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1128
Size: 1051 32 45
Fragments arranged by size:
1051 45 32
XmaI C'CCGG_G
Cuts at: 0 46 1128
Size: 46 1082
XmnI GAAnn'nnTTC
Cuts at: 0 236 1128
Size: 236 892
Enzymes that do cut:
AcuI AflIII AleI AlfI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbsI Bme1580I BmtI BplI
BpmI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI
Bsu36I BtsI DraIII DrdI EarI EcoICRI Eco57MI EcoO109I
FalI Hin4I HincII HindIII MfeI MluI MmeI MslI
MspA1I NheI PpuMI PspOMI PvuII SacI SapI SfcI
SmaI SrfI StyI TaqII TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AloI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmrI Bpu10I BpuEI BsaBI BsaHI BsaWI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I BtgI BtgZI ClaI CspCI DraI
EaeI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MscI NaeI NarI
NcoI NdeI NgoMIV NotI NruI NsiI NspI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PstI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI ZraI