Restriction map for
pF1KB5289
(Linear) (Six-base) MAPSORT of: KIBB5289.seq Check: 7511 from: 1 to: 1254
KIBB5289.orf
With 182 enzymes: SgfI *
October 7, 2009 14:16 ..
Acc65I G'GTAC_C
Cuts at: 0 358 1254
Size: 358 896
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 970 1254
Size: 934 36 284
Fragments arranged by size:
934 284 36
AlwNI CAG_nnn'CTG
Cuts at: 0 9 53 169 1254
Size: 9 44 116 1085
Fragments arranged by size:
1085 116 44 9
ApaI G_GGCC'C
Cuts at: 0 1215 1252 1254
Size: 1215 37 2
Fragments arranged by size:
1215 37 2
ApoI r'AATT_y
Cuts at: 0 593 852 1254
Size: 593 259 402
Fragments arranged by size:
593 402 259
AvaI C'yCGr_G
Cuts at: 0 40 682 1254
Size: 40 642 572
Fragments arranged by size:
642 572 40
BamHI G'GATC_C
Cuts at: 0 129 1254
Size: 129 1125
BanI G'GyrC_C
Cuts at: 0 358 465 1254
Size: 358 107 789
Fragments arranged by size:
789 358 107
BanII G_rGCy'C
Cuts at: 0 36 88 220 321 374 1215 1252 1254
Size: 36 52 132 101 53 841 37 2
Fragments arranged by size:
841 132 101 53 52 37 36 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 922 1254
Size: 922 332
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 213 247 1254
Size: 213 34 1007
Fragments arranged by size:
1007 213 34
BclI T'GATC_A
Cuts at: 0 335 1254
Size: 335 919
BglI GCCn_nnn'nGGC
Cuts at: 0 268 1254
Size: 268 986
BglII A'GATC_T
Cuts at: 0 771 1254
Size: 771 483
Bme1580I G_kGCm'C
Cuts at: 0 47 1215 1252 1254
Size: 47 1168 37 2
Fragments arranged by size:
1168 47 37 2
BmrI ACTGGGnnnn_n'
Cuts at: 0 555 1254
Size: 555 699
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 55 102 1254
Size: 55 47 1152
Fragments arranged by size:
1152 55 47
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 1254
Size: 651 603
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1254
Size: 391 863
BsaBI GATnn'nnATC
Cuts at: 0 770 1254
Size: 770 484
BsaWI w'CCGG_w
Cuts at: 0 805 1254
Size: 805 449
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 10 28 94 136 139 233 883 1254
Size: 10 18 66 42 3 94 650 371
Fragments arranged by size:
650 371 94 66 42 18 10 3
BseYI C'CCAG_C
Cuts at: 0 279 791 1117 1254
Size: 279 512 326 137
Fragments arranged by size:
512 326 279 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 943 1254
Size: 571 372 311
Fragments arranged by size:
571 372 311
BsiHKAI G_wGCw'C
Cuts at: 0 236 374 1254
Size: 236 138 880
Fragments arranged by size:
880 236 138
BsmI GAATG_Cn'
Cuts at: 0 611 1254
Size: 611 643
Bsp1286I G_dGCh'C
Cuts at: 0 36 47 88 220 236 321 374 1215
Size: 36 11 41 132 16 85 53 841
Cuts at: 1215 1252 1254
Size: 37 2
Fragments arranged by size:
841 132 85 53 41 37 36 16
11 2
BspHI T'CATG_A
Cuts at: 0 894 1254
Size: 894 360
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 536 1254
Size: 536 718
BsrBI CCG'CTC
Cuts at: 0 286 1254
Size: 286 968
BstXI CCAn_nnnn'nTGG
Cuts at: 0 462 1254
Size: 462 792
BstYI r'GATC_y
Cuts at: 0 129 771 1254
Size: 129 642 483
Fragments arranged by size:
642 483 129
Bsu36I CC'TnA_GG
Cuts at: 0 740 1254
Size: 740 514
BtgI C'CryG_G
Cuts at: 0 257 354 1254
Size: 257 97 900
Fragments arranged by size:
900 257 97
BtsI GCAGTG_nn'
Cuts at: 0 386 652 1254
Size: 386 266 602
Fragments arranged by size:
602 386 266
EaeI y'GGCC_r
Cuts at: 0 254 1254
Size: 254 1000
EarI CTCTTCn'nnn_
Cuts at: 0 698 1225 1254
Size: 698 527 29
Fragments arranged by size:
698 527 29
EcoICRI GAG'CTC
Cuts at: 0 372 1254
Size: 372 882
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 651 934 970 1254
Size: 651 283 36 284
Fragments arranged by size:
651 284 283 36
EcoO109I rG'GnC_Cy
Cuts at: 0 6 720 1211 1212 1248 1249 1254
Size: 6 714 491 1 36 1 5
Fragments arranged by size:
714 491 36 6 5 1 1
FspI TGC'GCA
Cuts at: 0 445 1254
Size: 445 809
HaeII r_GCGC'y
Cuts at: 0 115 1254
Size: 115 1139
KpnI G_GTAC'C
Cuts at: 0 362 1254
Size: 362 892
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 713 1254
Size: 200 513 541
Fragments arranged by size:
541 513 200
MscI TGG'CCA
Cuts at: 0 256 1254
Size: 256 998
MspA1I CmG'CkG
Cuts at: 0 53 166 779 795 1155 1254
Size: 53 113 613 16 360 99
Fragments arranged by size:
613 360 113 99 53 16
NcoI C'CATG_G
Cuts at: 0 354 1254
Size: 354 900
NdeI CA'TA_TG
Cuts at: 0 483 1254
Size: 483 771
PasI CC'CwG_GG
Cuts at: 0 1123 1244 1254
Size: 1123 121 10
Fragments arranged by size:
1123 121 10
PfoI T'CCnGG_A
Cuts at: 0 418 1254
Size: 418 836
PpuMI rG'GwC_Cy
Cuts at: 0 720 1254
Size: 720 534
PspOMI G'GGCC_C
Cuts at: 0 1211 1248 1254
Size: 1211 37 6
Fragments arranged by size:
1211 37 6
PspXI vC'TCGA_Gb
Cuts at: 0 682 1254
Size: 682 572
PstI C_TGCA'G
Cuts at: 0 989 1133 1254
Size: 989 144 121
Fragments arranged by size:
989 144 121
PvuII CAG'CTG
Cuts at: 0 53 779 795 1254
Size: 53 726 16 459
Fragments arranged by size:
726 459 53 16
SacI G_AGCT'C
Cuts at: 0 374 1254
Size: 374 880
SanDI GG'GwC_CC
Cuts at: 0 720 1254
Size: 720 534
SbfI CC_TGCA'GG
Cuts at: 0 989 1254
Size: 989 265
SfcI C'TryA_G
Cuts at: 0 985 1129 1254
Size: 985 144 125
Fragments arranged by size:
985 144 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 268 1254
Size: 268 986
SmlI C'TyrA_G
Cuts at: 0 406 682 1254
Size: 406 276 572
Fragments arranged by size:
572 406 276
StyI C'CwwG_G
Cuts at: 0 354 1254
Size: 354 900
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 537 920 1254
Size: 537 383 334
Fragments arranged by size:
537 383 334
Tth111I GACn'n_nGTC
Cuts at: 0 382 1254
Size: 382 872
XhoI C'TCGA_G
Cuts at: 0 682 1254
Size: 682 572
XmnI GAAnn'nnTTC
Cuts at: 0 675 1254
Size: 675 579
Enzymes that do cut:
Acc65I AcuI AlwNI ApaI ApoI AvaI BamHI BanI
BanII BbsI BcgI BclI BglI BglII Bme1580I BmrI
BplI BpmI BpuEI BsaBI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BstXI BstYI
Bsu36I BtgI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
FspI HaeII KpnI MmeI MscI MspA1I NcoI NdeI
PasI PfoI PpuMI PspOMI PspXI PstI PvuII SacI
SanDI SbfI SfcI SfiI SmlI StyI TaqII Tth111I
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BciVI BfrBI BlpI BmgBI
BmtI Bpu10I BsaI BsaAI BsaHI BsaXI BsiEI BsiWI
BsmBI BspEI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I BtgZI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspAI Hin4I HincII HindIII HpaI KasI MfeI MluI
MslI NaeI NarI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PsrI PvuI RsrII SacII SalI SapI ScaI
SexAI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI XbaI XcmI
XmaI ZraI