Restriction map for
pF1KB5288
(Linear) (Six-base) MAPSORT of: KIBB5288.seq Check: 2130 from: 1 to: 1650
KIBB5288.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 237 1650
Size: 237 1413
Acc65I G'GTAC_C
Cuts at: 0 901 1650
Size: 901 749
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1132 1650
Size: 1132 518
AfeI AGC'GCT
Cuts at: 0 301 1650
Size: 301 1349
AhdI GACnn_n'nnGTC
Cuts at: 0 1157 1650
Size: 1157 493
AleI CACnn'nnGTG
Cuts at: 0 351 1650
Size: 351 1299
AlwNI CAG_nnn'CTG
Cuts at: 0 165 252 1009 1650
Size: 165 87 757 641
Fragments arranged by size:
757 641 165 87
ApaI G_GGCC'C
Cuts at: 0 576 1650
Size: 576 1074
AvaI C'yCGr_G
Cuts at: 0 1052 1650
Size: 1052 598
AvrII C'CTAG_G
Cuts at: 0 1549 1650
Size: 1549 101
BanI G'GyrC_C
Cuts at: 0 901 1650
Size: 901 749
BanII G_rGCy'C
Cuts at: 0 576 1557 1650
Size: 576 981 93
Fragments arranged by size:
981 576 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 265 1650
Size: 265 1385
BbvCI CC'TCA_GC
Cuts at: 0 208 509 1580 1650
Size: 208 301 1071 70
Fragments arranged by size:
1071 301 208 70
BglI GCCn_nnn'nGGC
Cuts at: 0 1462 1650
Size: 1462 188
BlpI GC'TnA_GC
Cuts at: 0 296 866 1650
Size: 296 570 784
Fragments arranged by size:
784 570 296
Bme1580I G_kGCm'C
Cuts at: 0 576 945 1650
Size: 576 369 705
Fragments arranged by size:
705 576 369
BmrI ACTGGGnnnn_n'
Cuts at: 0 700 948 1019 1650
Size: 700 248 71 631
Fragments arranged by size:
700 631 248 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 88 187 1408 1621 1650
Size: 43 45 99 1221 213 29
Fragments arranged by size:
1221 213 99 45 43 29
Bpu10I CC'TnA_GC
Cuts at: 0 208 509 1102 1580 1650
Size: 208 301 593 478 70
Fragments arranged by size:
593 478 301 208 70
BsaI GGTCTCn'nnnn_
Cuts at: 0 1497 1650
Size: 1497 153
BsaWI w'CCGG_w
Cuts at: 0 107 338 1650
Size: 107 231 1312
Fragments arranged by size:
1312 231 107
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1177 1207 1248 1278 1650
Size: 1177 30 41 30 372
Fragments arranged by size:
1177 372 41 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 621 826 1650
Size: 621 205 824
Fragments arranged by size:
824 621 205
BseYI C'CCAG_C
Cuts at: 0 149 252 398 423 667 922 1367 1467
Size: 149 103 146 25 244 255 445 100
Cuts at: 1467 1650
Size: 183
Fragments arranged by size:
445 255 244 183 149 146 103 100
25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 934 1650
Size: 934 716
BsiEI CG_ry'CG
Cuts at: 0 318 1650
Size: 318 1332
BsiHKAI G_wGCw'C
Cuts at: 0 503 1650
Size: 503 1147
Bsp1286I G_dGCh'C
Cuts at: 0 503 576 945 1557 1650
Size: 503 73 369 612 93
Fragments arranged by size:
612 503 369 93 73
BspEI T'CCGG_A
Cuts at: 0 107 338 1650
Size: 107 231 1312
Fragments arranged by size:
1312 231 107
BspHI T'CATG_A
Cuts at: 0 807 1244 1650
Size: 807 437 406
Fragments arranged by size:
807 437 406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 237 1650
Size: 237 1413
BsrBI CCG'CTC
Cuts at: 0 629 1650
Size: 629 1021
BsrDI GCAATG_nn'
Cuts at: 0 501 1418 1650
Size: 501 917 232
Fragments arranged by size:
917 501 232
BsrFI r'CCGG_y
Cuts at: 0 266 986 1650
Size: 266 720 664
Fragments arranged by size:
720 664 266
BstXI CCAn_nnnn'nTGG
Cuts at: 0 353 1650
Size: 353 1297
BstYI r'GATC_y
Cuts at: 0 104 1650
Size: 104 1546
Bsu36I CC'TnA_GG
Cuts at: 0 633 907 995 1650
Size: 633 274 88 655
Fragments arranged by size:
655 633 274 88
BtgI C'CryG_G
Cuts at: 0 312 1591 1650
Size: 312 1279 59
Fragments arranged by size:
1279 312 59
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 638 1326 1650
Size: 638 688 324
Fragments arranged by size:
688 638 324
BtsI GCAGTG_nn'
Cuts at: 0 770 890 1168 1234 1650
Size: 770 120 278 66 416
Fragments arranged by size:
770 416 278 120 66
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 690 725 1650
Size: 690 35 925
Fragments arranged by size:
925 690 35
EaeI y'GGCC_r
Cuts at: 0 315 1650
Size: 315 1335
EagI C'GGCC_G
Cuts at: 0 315 1650
Size: 315 1335
EarI CTCTTCn'nnn_
Cuts at: 0 508 560 982 1010 1650
Size: 508 52 422 28 640
Fragments arranged by size:
640 508 422 52 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 88 187 1132 1408 1621 1650
Size: 43 45 99 945 276 213 29
Fragments arranged by size:
945 276 213 99 45 43 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 636 1650
Size: 636 1014
EcoO109I rG'GnC_Cy
Cuts at: 0 27 122 467 551 572 573 1301 1650
Size: 27 95 345 84 21 1 728 349
Fragments arranged by size:
728 349 345 95 84 27 21 1
HaeII r_GCGC'y
Cuts at: 0 303 1650
Size: 303 1347
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2 34 1143 1173 1175 1205 1650
Size: 2 32 1109 30 2 30 445
Fragments arranged by size:
1109 445 32 30 30 2 2
HincII GTy'rAC
Cuts at: 0 1041 1650
Size: 1041 609
KpnI G_GTAC'C
Cuts at: 0 905 1650
Size: 905 745
MslI CAynn'nnrTG
Cuts at: 0 351 448 1650
Size: 351 97 1202
Fragments arranged by size:
1202 351 97
MspA1I CmG'CkG
Cuts at: 0 231 295 314 398 423 1023 1332 1348
Size: 231 64 19 84 25 600 309 16
Cuts at: 1348 1371 1650
Size: 23 279
Fragments arranged by size:
600 309 279 231 84 64 25 23
19 16
NaeI GCC'GGC
Cuts at: 0 268 1650
Size: 268 1382
NgoMIV G'CCGG_C
Cuts at: 0 266 1650
Size: 266 1384
PasI CC'CwG_GG
Cuts at: 0 621 1496 1650
Size: 621 875 154
Fragments arranged by size:
875 621 154
PflMI CCAn_nnn'nTGG
Cuts at: 0 712 735 1287 1317 1650
Size: 712 23 552 30 333
Fragments arranged by size:
712 552 333 30 23
PfoI T'CCnGG_A
Cuts at: 0 20 437 1650
Size: 20 417 1213
Fragments arranged by size:
1213 417 20
PpuMI rG'GwC_Cy
Cuts at: 0 27 467 1301 1650
Size: 27 440 834 349
Fragments arranged by size:
834 440 349 27
PshAI GACnn'nnGTC
Cuts at: 0 1054 1650
Size: 1054 596
PspOMI G'GGCC_C
Cuts at: 0 572 1650
Size: 572 1078
PspXI vC'TCGA_Gb
Cuts at: 0 1052 1650
Size: 1052 598
PstI C_TGCA'G
Cuts at: 0 230 422 1175 1612 1650
Size: 230 192 753 437 38
Fragments arranged by size:
753 437 230 192 38
PvuII CAG'CTG
Cuts at: 0 423 1650
Size: 423 1227
SacII CC_GC'GG
Cuts at: 0 315 1650
Size: 315 1335
SapI GCTCTTCn'nnn_
Cuts at: 0 508 1010 1650
Size: 508 502 640
Fragments arranged by size:
640 508 502
SfcI C'TryA_G
Cuts at: 0 226 418 1171 1608 1650
Size: 226 192 753 437 42
Fragments arranged by size:
753 437 226 192 42
SmlI C'TyrA_G
Cuts at: 0 1052 1650
Size: 1052 598
SspI AAT'ATT
Cuts at: 0 370 1650
Size: 370 1280
StuI AGG'CCT
Cuts at: 0 1604 1650
Size: 1604 46
StyI C'CwwG_G
Cuts at: 0 1549 1650
Size: 1549 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 1650
Size: 1177 32 441
Fragments arranged by size:
1177 441 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 753 1475 1650
Size: 753 722 175
Fragments arranged by size:
753 722 175
XhoI C'TCGA_G
Cuts at: 0 1052 1650
Size: 1052 598
XmnI GAAnn'nnTTC
Cuts at: 0 1515 1650
Size: 1515 135
Enzymes that do cut:
AarI Acc65I AcuI AfeI AhdI AleI AlwNI ApaI
AvaI AvrII BanI BanII BbsI BbvCI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EagI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I HincII KpnI MslI MspA1I NaeI NgoMIV
PasI PflMI PfoI PpuMI PshAI PspOMI PspXI PstI
PvuII SacII SapI SfcI SmlI SspI StuI StyI
TstI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AlfI AloI
ApaLI ApoI AscI AseI AsiSI BaeI BamHI BbeI
BcgI BciVI BclI BfrBI BglII BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsiWI BsmI BsmBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I ClaI DraI
DraIII DrdI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HindIII HpaI KasI MfeI MluI MmeI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PsiI PsrI
PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SwaI TaqII TatI Tth111I XbaI XmaI ZraI