Restriction map for
pF1KB5209
(Linear) (Six-base) MAPSORT of: KIBB5209.seq Check: 3402 from: 1 to: 1581
KIBB5209.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 1581
Size: 250 1331
AflIII A'CryG_T
Cuts at: 0 302 672 1094 1581
Size: 302 370 422 487
Fragments arranged by size:
487 422 370 302
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 111 1177 1201 1351 1557 1581
Size: 111 1066 24 150 206 24
Fragments arranged by size:
1066 206 150 111 24 24
AlwNI CAG_nnn'CTG
Cuts at: 0 852 1581
Size: 852 729
ApaI G_GGCC'C
Cuts at: 0 703 1504 1581
Size: 703 801 77
Fragments arranged by size:
801 703 77
ApoI r'AATT_y
Cuts at: 0 964 1581
Size: 964 617
BamHI G'GATC_C
Cuts at: 0 1419 1581
Size: 1419 162
BanI G'GyrC_C
Cuts at: 0 95 1514 1581
Size: 95 1419 67
Fragments arranged by size:
1419 95 67
BanII G_rGCy'C
Cuts at: 0 85 703 911 1388 1504 1581
Size: 85 618 208 477 116 77
Fragments arranged by size:
618 477 208 116 85 77
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 280 1581
Size: 115 165 1301
Fragments arranged by size:
1301 165 115
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1533 1567 1581
Size: 1533 34 14
Fragments arranged by size:
1533 34 14
BclI T'GATC_A
Cuts at: 0 1362 1581
Size: 1362 219
BglI GCCn_nnn'nGGC
Cuts at: 0 32 868 1581
Size: 32 836 713
Fragments arranged by size:
836 713 32
BglII A'GATC_T
Cuts at: 0 797 1581
Size: 797 784
Bme1580I G_kGCm'C
Cuts at: 0 703 1504 1581
Size: 703 801 77
Fragments arranged by size:
801 703 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 1533 1581
Size: 1533 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 411 1581
Size: 411 1170
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 481 1147 1297 1581
Size: 406 75 666 150 284
Fragments arranged by size:
666 406 284 150 75
Bpu10I CC'TnA_GC
Cuts at: 0 1142 1581
Size: 1142 439
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1581
Size: 1336 245
BsaAI yAC'GTr
Cuts at: 0 1097 1206 1581
Size: 1097 109 375
Fragments arranged by size:
1097 375 109
BsaBI GATnn'nnATC
Cuts at: 0 1399 1581
Size: 1399 182
BsaHI Gr'CG_yC
Cuts at: 0 115 1581
Size: 115 1466
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1090 1120 1581
Size: 1090 30 461
Fragments arranged by size:
1090 461 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 262 265 520 523 697 1144 1581
Size: 262 3 255 3 174 447 437
Fragments arranged by size:
447 437 262 255 174 3 3
BseYI C'CCAG_C
Cuts at: 0 566 930 1581
Size: 566 364 651
Fragments arranged by size:
651 566 364
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 1173 1175 1581
Size: 1147 26 2 406
Fragments arranged by size:
1147 406 26 2
BsiHKAI G_wGCw'C
Cuts at: 0 397 1048 1581
Size: 397 651 533
Fragments arranged by size:
651 533 397
BsiWI C'GTAC_G
Cuts at: 0 1097 1581
Size: 1097 484
BsmI GAATG_Cn'
Cuts at: 0 76 602 1581
Size: 76 526 979
Fragments arranged by size:
979 526 76
Bsp1286I G_dGCh'C
Cuts at: 0 85 397 703 911 1048 1388 1504 1581
Size: 85 312 306 208 137 340 116 77
Fragments arranged by size:
340 312 306 208 137 116 85 77
BsrDI GCAATG_nn'
Cuts at: 0 1551 1557 1581
Size: 1551 6 24
Fragments arranged by size:
1551 24 6
BsrFI r'CCGG_y
Cuts at: 0 46 1581
Size: 46 1535
BssSI C'ACGA_G
Cuts at: 0 922 1581
Size: 922 659
BstAPI GCAn_nnn'nTGC
Cuts at: 0 144 1581
Size: 144 1437
BstEII G'GTnAC_C
Cuts at: 0 981 1581
Size: 981 600
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 1125 1511 1581
Size: 30 1095 386 70
Fragments arranged by size:
1095 386 70 30
BstYI r'GATC_y
Cuts at: 0 254 473 797 1301 1400 1419 1581
Size: 254 219 324 504 99 19 162
Fragments arranged by size:
504 324 254 219 162 99 19
Bsu36I CC'TnA_GG
Cuts at: 0 542 1581
Size: 542 1039
BtsI GCAGTG_nn'
Cuts at: 0 95 137 138 1363 1581
Size: 95 42 1 1225 218
Fragments arranged by size:
1225 218 95 42 1
EaeI y'GGCC_r
Cuts at: 0 20 515 710 1581
Size: 20 495 195 871
Fragments arranged by size:
871 495 195 20
EarI CTCTTCn'nnn_
Cuts at: 0 16 1067 1581
Size: 16 1051 514
Fragments arranged by size:
1051 514 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 406 481 1147 1297 1581
Size: 250 156 75 666 150 284
Fragments arranged by size:
666 284 250 156 150 75
EcoNI CCTnn'n_nnAGG
Cuts at: 0 973 1126 1408 1581
Size: 973 153 282 173
Fragments arranged by size:
973 282 173 153
EcoO109I rG'GnC_Cy
Cuts at: 0 700 927 1500 1581
Size: 700 227 573 81
Fragments arranged by size:
700 573 227 81
EcoRI G'AATT_C
Cuts at: 0 964 1581
Size: 964 617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 1049 1581
Size: 143 906 532
Fragments arranged by size:
906 532 143
MscI TGG'CCA
Cuts at: 0 22 517 1581
Size: 22 495 1064
Fragments arranged by size:
1064 495 22
MslI CAynn'nnrTG
Cuts at: 0 607 1581
Size: 607 974
MspA1I CmG'CkG
Cuts at: 0 217 459 1581
Size: 217 242 1122
Fragments arranged by size:
1122 242 217
NaeI GCC'GGC
Cuts at: 0 48 1581
Size: 48 1533
NdeI CA'TA_TG
Cuts at: 0 193 718 1581
Size: 193 525 863
Fragments arranged by size:
863 525 193
NgoMIV G'CCGG_C
Cuts at: 0 46 1581
Size: 46 1535
NspI r_CATG'y
Cuts at: 0 306 676 777 1161 1581
Size: 306 370 101 384 420
Fragments arranged by size:
420 384 370 306 101
PciI A'CATG_T
Cuts at: 0 302 672 1581
Size: 302 370 909
Fragments arranged by size:
909 370 302
PflMI CCAn_nnn'nTGG
Cuts at: 0 663 1086 1581
Size: 663 423 495
Fragments arranged by size:
663 495 423
PfoI T'CCnGG_A
Cuts at: 0 350 1581
Size: 350 1231
PmlI CAC'GTG
Cuts at: 0 1206 1581
Size: 1206 375
PpuMI rG'GwC_Cy
Cuts at: 0 927 1581
Size: 927 654
PshAI GACnn'nnGTC
Cuts at: 0 853 1581
Size: 853 728
PspOMI G'GGCC_C
Cuts at: 0 699 1500 1581
Size: 699 801 81
Fragments arranged by size:
801 699 81
PstI C_TGCA'G
Cuts at: 0 149 1330 1581
Size: 149 1181 251
Fragments arranged by size:
1181 251 149
PvuII CAG'CTG
Cuts at: 0 217 459 1581
Size: 217 242 1122
Fragments arranged by size:
1122 242 217
SfcI C'TryA_G
Cuts at: 0 145 1326 1581
Size: 145 1181 255
Fragments arranged by size:
1181 255 145
SmlI C'TyrA_G
Cuts at: 0 1315 1581
Size: 1315 266
SphI G_CATG'C
Cuts at: 0 1161 1581
Size: 1161 420
StuI AGG'CCT
Cuts at: 0 661 1581
Size: 661 920
StyI C'CwwG_G
Cuts at: 0 57 662 791 1581
Size: 57 605 129 790
Fragments arranged by size:
790 605 129 57
Tth111I GACn'n_nGTC
Cuts at: 0 114 205 1581
Size: 114 91 1376
Fragments arranged by size:
1376 114 91
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 849 1581
Size: 849 732
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaI ApoI BamHI BanI
BanII BbsI BcgI BclI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I BsrDI
BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtsI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI MmeI MscI
MslI MspA1I NaeI NdeI NgoMIV NspI PciI PflMI
PfoI PmlI PpuMI PshAI PspOMI PstI PvuII SfcI
SmlI SphI StuI StyI Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbvCI BciVI BfrBI BlpI BmgBI
BmtI BsaI BsaWI BsiEI BsmBI BspEI BspHI BspMI
BsrBI BsrGI BssHII BstBI BstZ17I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoRV
FalI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI NarI NcoI NheI
NotI NruI NsiI PacI PasI PmeI PpiI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TaqII TatI
TstI XbaI XhoI XmaI XmnI ZraI