Restriction map for
pF1KB5204
(Linear) (Six-base) MAPSORT of: KIBB5204.seq Check: 940 from: 1 to: 807
KIBB5204.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 351 807
Size: 351 456
AleI CACnn'nnGTG
Cuts at: 0 179 807
Size: 179 628
AlwNI CAG_nnn'CTG
Cuts at: 0 66 81 807
Size: 66 15 726
Fragments arranged by size:
726 66 15
ApaI G_GGCC'C
Cuts at: 0 479 807
Size: 479 328
ApoI r'AATT_y
Cuts at: 0 124 487 807
Size: 124 363 320
Fragments arranged by size:
363 320 124
AvaI C'yCGr_G
Cuts at: 0 280 349 807
Size: 280 69 458
Fragments arranged by size:
458 280 69
BanI G'GyrC_C
Cuts at: 0 416 807
Size: 416 391
BanII G_rGCy'C
Cuts at: 0 479 807
Size: 479 328
BbsI GAAGACnn'nnnn_
Cuts at: 0 196 807
Size: 196 611
Bme1580I G_kGCm'C
Cuts at: 0 479 785 807
Size: 479 306 22
Fragments arranged by size:
479 306 22
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 495 807
Size: 495 312
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 739 807
Size: 545 194 68
Fragments arranged by size:
545 194 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 280 310 543 573 807
Size: 280 30 233 30 234
Fragments arranged by size:
280 234 233 30 30
BseYI C'CCAG_C
Cuts at: 0 66 807
Size: 66 741
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 333 807
Size: 322 11 474
Fragments arranged by size:
474 322 11
Bsp1286I G_dGCh'C
Cuts at: 0 479 785 807
Size: 479 306 22
Fragments arranged by size:
479 306 22
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 648 714 807
Size: 648 66 93
Fragments arranged by size:
648 93 66
BsrFI r'CCGG_y
Cuts at: 0 419 807
Size: 419 388
BssSI C'ACGA_G
Cuts at: 0 454 480 807
Size: 454 26 327
Fragments arranged by size:
454 327 26
BstAPI GCAn_nnn'nTGC
Cuts at: 0 81 807
Size: 81 726
BstEII G'GTnAC_C
Cuts at: 0 726 807
Size: 726 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 181 807
Size: 181 626
BtgI C'CryG_G
Cuts at: 0 384 515 807
Size: 384 131 292
Fragments arranged by size:
384 292 131
BtsI GCAGTG_nn'
Cuts at: 0 538 807
Size: 538 269
EaeI y'GGCC_r
Cuts at: 0 712 807
Size: 712 95
EarI CTCTTCn'nnn_
Cuts at: 0 636 807
Size: 636 171
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 572 807
Size: 572 235
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 545 739 807
Size: 545 194 68
Fragments arranged by size:
545 194 68
EcoO109I rG'GnC_Cy
Cuts at: 0 475 476 706 807
Size: 475 1 230 101
Fragments arranged by size:
475 230 101 1
EcoRI G'AATT_C
Cuts at: 0 124 807
Size: 124 683
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 57 807
Size: 57 750
HaeII r_GCGC'y
Cuts at: 0 113 807
Size: 113 694
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 337 369 543 575 807
Size: 337 32 174 32 232
Fragments arranged by size:
337 232 174 32 32
HincII GTy'rAC
Cuts at: 0 617 807
Size: 617 190
HpaI GTT'AAC
Cuts at: 0 617 807
Size: 617 190
MslI CAynn'nnrTG
Cuts at: 0 179 260 442 807
Size: 179 81 182 365
Fragments arranged by size:
365 182 179 81
MspA1I CmG'CkG
Cuts at: 0 66 81 807
Size: 66 15 726
Fragments arranged by size:
726 66 15
NcoI C'CATG_G
Cuts at: 0 515 807
Size: 515 292
NspI r_CATG'y
Cuts at: 0 162 807
Size: 162 645
PpuMI rG'GwC_Cy
Cuts at: 0 706 807
Size: 706 101
PspOMI G'GGCC_C
Cuts at: 0 475 807
Size: 475 332
PstI C_TGCA'G
Cuts at: 0 415 807
Size: 415 392
PvuII CAG'CTG
Cuts at: 0 66 807
Size: 66 741
SanDI GG'GwC_CC
Cuts at: 0 706 807
Size: 706 101
SfcI C'TryA_G
Cuts at: 0 411 807
Size: 411 396
StyI C'CwwG_G
Cuts at: 0 373 515 807
Size: 373 142 292
Fragments arranged by size:
373 292 142
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 783 807
Size: 783 24
Enzymes that do cut:
AhdI AleI AlwNI ApaI ApoI AvaI BanI BanII
BbsI Bme1580I BplI BpmI BsaXI BseYI BsgI Bsp1286I
BspMI BsrFI BssSI BstAPI BstEII BstXI BtgI BtsI
EaeI EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII
Hin4I HincII HpaI MslI MspA1I NcoI NspI PpuMI
PspOMI PstI PvuII SanDI SfcI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI BmgBI BmrI BmtI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrGI BssHII BstBI BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoICRI EcoNI EcoRV
FseI FspI FspAI HindIII KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI