(Linear) (Six-base) MAPSORT of: KIBB5163.seq  Check: 5504  from: 1  to: 624



KIBB5163.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AflIII A'CryG_T

Cuts at:      0     504     624 
   Size:        504     120
 
ApoI r'AATT_y

Cuts at:      0      37     624 
   Size:         37     587
 
BbvCI CC'TCA_GC

Cuts at:      0      31     624 
   Size:         31     593
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     481     515     624 
   Size:        481      34     109

  Fragments arranged by size: 

                 481     109      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     472     624 
   Size:        472     152
 
BclI T'GATC_A

Cuts at:      0      86     624 
   Size:         86     538
 
BglII A'GATC_T

Cuts at:      0     384     624 
   Size:        384     240
 
BmgBI CAC'GTC

Cuts at:      0     505     624 
   Size:        505     119
 
Bpu10I CC'TnA_GC

Cuts at:      0      31     624 
   Size:         31     593
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     430     624 
   Size:        430     194
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     199     624 
   Size:        199     425
 
BsrBI CCG'CTC

Cuts at:      0     219     624 
   Size:        219     405
 
BstYI r'GATC_y

Cuts at:      0     384     624 
   Size:        384     240
 
Bsu36I CC'TnA_GG

Cuts at:      0     587     624 
   Size:        587      37
 
BtgI C'CryG_G

Cuts at:      0       5     624 
   Size:          5     619
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      26     624 
   Size:         26     598
 
EcoRV GAT'ATC

Cuts at:      0     282     624 
   Size:        282     342
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     513     624 
   Size:        513     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168     624 
   Size:        136      32     456

  Fragments arranged by size: 

                 456     136      32
 
HincII GTy'rAC

Cuts at:      0     294     624 
   Size:        294     330
 
HpaI GTT'AAC

Cuts at:      0     294     624 
   Size:        294     330
 
MfeI C'AATT_G

Cuts at:      0     134     410     624 
   Size:        134     276     214

  Fragments arranged by size: 

                 276     214     134
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     624 
   Size:         51     573
 
MspA1I CmG'CkG

Cuts at:      0      30     624 
   Size:         30     594
 
SpeI A'CTAG_T

Cuts at:      0     463     624 
   Size:        463     161
 
TatI w'GTAC_w

Cuts at:      0     164     624 
   Size:        164     460
 

 Enzymes that do cut:

   AflIII     ApoI    BbvCI     BcgI    BciVI     BclI    BglII    BmgBI
   Bpu10I    BseRI    BspMI    BsrBI    BstYI   Bsu36I     BtgI     EciI
    EcoRV     FalI    Hin4I   HincII     HpaI     MfeI     MmeI   MspA1I
     SpeI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BfrBI     BglI     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MluI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI