(Linear) (Six-base) MAPSORT of: KIBB5161.seq  Check: 1318  from: 1  to: 1389



KIBB5161.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     620    1079    1389 
   Size:        620     459     310

  Fragments arranged by size: 

                 620     459     310
 
AflIII A'CryG_T

Cuts at:      0    1359    1389 
   Size:       1359      30
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     641     673    1389 
   Size:        641      32     716

  Fragments arranged by size: 

                 716     641      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      93    1389 
   Size:         93    1296
 
ApoI r'AATT_y

Cuts at:      0    1372    1389 
   Size:       1372      17
 
AseI AT'TA_AT

Cuts at:      0     544    1389 
   Size:        544     845
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1220    1253    1389 
   Size:       1220      33     136

  Fragments arranged by size: 

                1220     136      33
 
BglI GCCn_nnn'nGGC

Cuts at:      0      76    1389 
   Size:         76    1313
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     643    1007    1389 
   Size:        356     287     364     382

  Fragments arranged by size: 

                 382     364     356     287
 
Bpu10I CC'TnA_GC

Cuts at:      0      86     914    1247    1344    1389 
   Size:         86     828     333      97      45

  Fragments arranged by size: 

                 828     333      97      86      45
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     641     671    1389 
   Size:        641      30     718

  Fragments arranged by size: 

                 718     641      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     365    1389 
   Size:        365    1024
 
BseYI C'CCAG_C

Cuts at:      0      71    1389 
   Size:         71    1318
 
BsrFI r'CCGG_y

Cuts at:      0      56    1389 
   Size:         56    1333
 
BssSI C'ACGA_G

Cuts at:      0    1232    1389 
   Size:       1232     157
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     452    1389 
   Size:        452     937
 
BtgI C'CryG_G

Cuts at:      0      35     120    1389 
   Size:         35      85    1269

  Fragments arranged by size: 

                1269      85      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     562    1389 
   Size:        562     827
 
EaeI y'GGCC_r

Cuts at:      0      32      38     986    1389 
   Size:         32       6     948     403

  Fragments arranged by size: 

                 948     403      32       6
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     356     620     643    1007    1079    1389 
   Size:        356     264      23     364      72     310

  Fragments arranged by size: 

                 364     356     310     264      72      23
 
EcoO109I rG'GnC_Cy

Cuts at:      0     535    1389 
   Size:        535     854
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     879    1389 
   Size:        879     510
 
HincII GTy'rAC

Cuts at:      0     570    1125    1389 
   Size:        570     555     264

  Fragments arranged by size: 

                 570     555     264
 
HindIII A'AGCT_T

Cuts at:      0     245     724    1084    1389 
   Size:        245     479     360     305

  Fragments arranged by size: 

                 479     360     305     245
 
MfeI C'AATT_G

Cuts at:      0     254    1389 
   Size:        254    1135
 
MscI TGG'CCA

Cuts at:      0      40     988    1389 
   Size:         40     948     401

  Fragments arranged by size: 

                 948     401      40
 
MslI CAynn'nnrTG

Cuts at:      0     178     563    1364    1389 
   Size:        178     385     801      25

  Fragments arranged by size: 

                 801     385     178      25
 
MspA1I CmG'CkG

Cuts at:      0      75    1389 
   Size:         75    1314
 
NcoI C'CATG_G

Cuts at:      0     120    1389 
   Size:        120    1269
 
NspI r_CATG'y

Cuts at:      0    1363    1389 
   Size:       1363      26
 
PciI A'CATG_T

Cuts at:      0    1359    1389 
   Size:       1359      30
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     641     673    1389 
   Size:        641      32     716

  Fragments arranged by size: 

                 716     641      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     535    1389 
   Size:        535     854
 
SfcI C'TryA_G

Cuts at:      0     871    1055    1389 
   Size:        871     184     334

  Fragments arranged by size: 

                 871     334     184
 
SspI AAT'ATT

Cuts at:      0     693    1389 
   Size:        693     696
 
StyI C'CwwG_G

Cuts at:      0     120    1389 
   Size:        120    1269
 
TatI w'GTAC_w

Cuts at:      0     132    1389 
   Size:        132    1257
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     222    1389 
   Size:        222    1167
 

 Enzymes that do cut:

     AcuI   AflIII     AloI    AlwNI     ApoI     AseI     BaeI     BglI
     BpmI   Bpu10I    BsaXI    BseRI    BseYI    BsrFI    BssSI   BstAPI
     BtgI   DraIII     EaeI  Eco57MI EcoO109I     FalI   HincII  HindIII
     MfeI     MscI     MslI   MspA1I     NcoI     NspI     PciI     PpiI
    PpuMI     SfcI     SspI     StyI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     ApaI    ApaLI     AscI    AsiSI     AvaI
    AvrII    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrGI   BssHII    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I     HpaI     KasI
     KpnI     MluI     MmeI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI     PmeI
     PmlI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI