(Linear) (Six-base) MAPSORT of: KIBB5158.seq  Check: 3672  from: 1  to: 750



KIBB5158.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:04  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     244     750 
   Size:        244     506
 
AflIII A'CryG_T

Cuts at:      0     621     750 
   Size:        621     129
 
ApoI r'AATT_y

Cuts at:      0     241     750 
   Size:        241     509
 
BfrBI ATG'CAT

Cuts at:      0     391     750 
   Size:        391     359
 
BlpI GC'TnA_GC

Cuts at:      0      32     129     750 
   Size:         32      97     621

  Fragments arranged by size: 

                 621      97      32
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     290     750 
   Size:        290     460
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      66     351     750 
   Size:         66     285     399

  Fragments arranged by size: 

                 399     285      66
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     202     750 
   Size:         58     144     548

  Fragments arranged by size: 

                 548     144      58
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     238     750 
   Size:        238     512
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     132     162     750 
   Size:        132      30     588

  Fragments arranged by size: 

                 588     132      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      24      55     750 
   Size:         24      31     695

  Fragments arranged by size: 

                 695      31      24
 
BsmI GAATG_Cn'

Cuts at:      0     295     750 
   Size:        295     455
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     152     750 
   Size:        152     598
 
BspHI T'CATG_A

Cuts at:      0     110     750 
   Size:        110     640
 
BsrBI CCG'CTC

Cuts at:      0     105     750 
   Size:        105     645
 
BsrGI T'GTAC_A

Cuts at:      0     583     750 
   Size:        583     167
 
BstYI r'GATC_y

Cuts at:      0     263     750 
   Size:        263     487
 
EaeI y'GGCC_r

Cuts at:      0     188     750 
   Size:        188     562
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      58     202     244     750 
   Size:         58     144      42     506

  Fragments arranged by size: 

                 506     144      58      42
 
MfeI C'AATT_G

Cuts at:      0     483     750 
   Size:        483     267
 
MscI TGG'CCA

Cuts at:      0     190     750 
   Size:        190     560
 
MspA1I CmG'CkG

Cuts at:      0      36     133     750 
   Size:         36      97     617

  Fragments arranged by size: 

                 617      97      36
 
NdeI CA'TA_TG

Cuts at:      0     369     750 
   Size:        369     381
 
NsiI A_TGCA'T

Cuts at:      0     393     750 
   Size:        393     357
 
PvuII CAG'CTG

Cuts at:      0      36     133     750 
   Size:         36      97     617

  Fragments arranged by size: 

                 617      97      36
 
SfcI C'TryA_G

Cuts at:      0      53     750 
   Size:         53     697
 
SmlI C'TyrA_G

Cuts at:      0     217     750 
   Size:        217     533
 
TatI w'GTAC_w

Cuts at:      0     468     583     750 
   Size:        468     115     167

  Fragments arranged by size: 

                 468     167     115
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI    BfrBI     BlpI     BmrI     BplI     BpmI
    BpuEI    BsaXI    BseRI     BsmI    BsmBI    BspHI    BsrBI    BsrGI
    BstYI     EaeI  Eco57MI     MfeI     MscI   MspA1I     NdeI     NsiI
    PvuII     SfcI     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI     BglI    BglII
 Bme1580I    BmgBI     BmtI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I    BspEI
    BspMI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI     MmeI
     MslI     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI