Restriction map for
pF1KB5126
(Linear) (Six-base) MAPSORT of: KIBB5126.seq Check: 6646 from: 1 to: 1026
KIBB5126.orf
With 182 enzymes: SgfI *
April 24, 2009 13:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 459 1026
Size: 459 567
AccI GT'mk_AC
Cuts at: 0 385 1026
Size: 385 641
Acc65I G'GTAC_C
Cuts at: 0 104 1026
Size: 104 922
AgeI A'CCGG_T
Cuts at: 0 107 1026
Size: 107 919
AhdI GACnn_n'nnGTC
Cuts at: 0 381 1026
Size: 381 645
AlwNI CAG_nnn'CTG
Cuts at: 0 304 1003 1026
Size: 304 699 23
Fragments arranged by size:
699 304 23
ApoI r'AATT_y
Cuts at: 0 572 1026
Size: 572 454
AvaI C'yCGr_G
Cuts at: 0 41 130 1026
Size: 41 89 896
Fragments arranged by size:
896 89 41
BanI G'GyrC_C
Cuts at: 0 104 1026
Size: 104 922
BanII G_rGCy'C
Cuts at: 0 406 495 751 1026
Size: 406 89 256 275
Fragments arranged by size:
406 275 256 89
BbsI GAAGACnn'nnnn_
Cuts at: 0 833 1026
Size: 833 193
BbvCI CC'TCA_GC
Cuts at: 0 665 1026
Size: 665 361
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 283 317 1026
Size: 283 34 709
Fragments arranged by size:
709 283 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 579 1026
Size: 579 447
BlpI GC'TnA_GC
Cuts at: 0 464 750 1026
Size: 464 286 276
Fragments arranged by size:
464 286 276
Bme1580I G_kGCm'C
Cuts at: 0 32 983 1026
Size: 32 951 43
Fragments arranged by size:
951 43 32
BmrI ACTGGGnnnn_n'
Cuts at: 0 759 785 1026
Size: 759 26 241
Fragments arranged by size:
759 241 26
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 263 1026
Size: 123 140 763
Fragments arranged by size:
763 140 123
Bpu10I CC'TnA_GC
Cuts at: 0 503 665 745 1026
Size: 503 162 80 281
Fragments arranged by size:
503 281 162 80
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 837 1026
Size: 420 417 189
Fragments arranged by size:
420 417 189
BsaWI w'CCGG_w
Cuts at: 0 107 1026
Size: 107 919
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 118 518 1026
Size: 118 400 508
Fragments arranged by size:
508 400 118
BseYI C'CCAG_C
Cuts at: 0 158 950 996 1026
Size: 158 792 46 30
Fragments arranged by size:
792 158 46 30
BsiHKAI G_wGCw'C
Cuts at: 0 406 495 751 1026
Size: 406 89 256 275
Fragments arranged by size:
406 275 256 89
Bsp1286I G_dGCh'C
Cuts at: 0 32 406 495 751 983 1026
Size: 32 374 89 256 232 43
Fragments arranged by size:
374 256 232 89 43 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 459 1017 1026
Size: 459 558 9
Fragments arranged by size:
558 459 9
BsrBI CCG'CTC
Cuts at: 0 921 1026
Size: 921 105
BsrFI r'CCGG_y
Cuts at: 0 107 163 1026
Size: 107 56 863
Fragments arranged by size:
863 107 56
BsrGI T'GTAC_A
Cuts at: 0 756 1026
Size: 756 270
BstAPI GCAn_nnn'nTGC
Cuts at: 0 356 1026
Size: 356 670
BtsI GCAGTG_nn'
Cuts at: 0 630 1026
Size: 630 396
DraIII CAC_nnn'GTG
Cuts at: 0 799 978 1026
Size: 799 179 48
Fragments arranged by size:
799 179 48
DrdI GACnn_nn'nnGTC
Cuts at: 0 961 1026
Size: 961 65
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 210 1026
Size: 210 816
EcoICRI GAG'CTC
Cuts at: 0 404 493 749 1026
Size: 404 89 256 277
Fragments arranged by size:
404 277 256 89
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 123 263 1026
Size: 123 140 763
Fragments arranged by size:
763 140 123
EcoO109I rG'GnC_Cy
Cuts at: 0 134 186 456 1026
Size: 134 52 270 570
Fragments arranged by size:
570 270 134 52
HaeII r_GCGC'y
Cuts at: 0 124 156 1026
Size: 124 32 870
Fragments arranged by size:
870 124 32
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 367 399 1026
Size: 367 32 627
Fragments arranged by size:
627 367 32
KpnI G_GTAC'C
Cuts at: 0 108 1026
Size: 108 918
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 71 1026
Size: 71 955
MspA1I CmG'CkG
Cuts at: 0 593 754 1026
Size: 593 161 272
Fragments arranged by size:
593 272 161
NaeI GCC'GGC
Cuts at: 0 165 1026
Size: 165 861
NgoMIV G'CCGG_C
Cuts at: 0 163 1026
Size: 163 863
PflMI CCAn_nnn'nTGG
Cuts at: 0 1003 1026
Size: 1003 23
PpuMI rG'GwC_Cy
Cuts at: 0 186 456 1026
Size: 186 270 570
Fragments arranged by size:
570 270 186
PstI C_TGCA'G
Cuts at: 0 625 1026
Size: 625 401
PvuII CAG'CTG
Cuts at: 0 754 1026
Size: 754 272
RsrII CG'GwC_CG
Cuts at: 0 110 1026
Size: 110 916
SacI G_AGCT'C
Cuts at: 0 406 495 751 1026
Size: 406 89 256 275
Fragments arranged by size:
406 275 256 89
SfcI C'TryA_G
Cuts at: 0 386 621 1026
Size: 386 235 405
Fragments arranged by size:
405 386 235
SmaI CCC'GGG
Cuts at: 0 43 1026
Size: 43 983
SmlI C'TyrA_G
Cuts at: 0 399 852 1026
Size: 399 453 174
Fragments arranged by size:
453 399 174
TatI w'GTAC_w
Cuts at: 0 730 756 1026
Size: 730 26 270
Fragments arranged by size:
730 270 26
Tth111I GACn'n_nGTC
Cuts at: 0 912 1026
Size: 912 114
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 238 772 1026
Size: 238 534 254
Fragments arranged by size:
534 254 238
XmaI C'CCGG_G
Cuts at: 0 41 1026
Size: 41 985
Enzymes that do cut:
AarI AccI Acc65I AgeI AhdI AlwNI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BciVI BlpI Bme1580I
BmrI BpmI Bpu10I BpuEI BsaWI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BtsI DraIII
DrdI EciI EcoICRI Eco57MI EcoO109I HaeII Hin4I KpnI
MmeI MspA1I NaeI NgoMIV PflMI PpuMI PstI PvuII
RsrII SacI SfcI SmaI SmlI TatI Tth111I XcmI
XmaI
Enzymes that do not cut:
AatII AclI AcuI AfeI AflII AflIII AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BclI BfrBI BglI BglII BmgBI BmtI
BplI BsaI BsaAI BsaBI BsaHI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI EaeI EagI EarI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI MscI MslI NarI NcoI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PsrI PvuI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TaqII TstI XbaI
XhoI XmnI ZraI