Restriction map for
pF1KB5055
(Linear) (Six-base) MAPSORT of: KIBB5055.seq Check: 7362 from: 1 to: 1542
KIBB5055.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 937 1542
Size: 937 605
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 700 1542
Size: 601 99 842
Fragments arranged by size:
842 601 99
AflII C'TTAA_G
Cuts at: 0 181 1462 1542
Size: 181 1281 80
Fragments arranged by size:
1281 181 80
AhdI GACnn_n'nnGTC
Cuts at: 0 1350 1542
Size: 1350 192
AlwNI CAG_nnn'CTG
Cuts at: 0 226 352 732 1542
Size: 226 126 380 810
Fragments arranged by size:
810 380 226 126
ApoI r'AATT_y
Cuts at: 0 292 596 834 1363 1542
Size: 292 304 238 529 179
Fragments arranged by size:
529 304 292 238 179
BamHI G'GATC_C
Cuts at: 0 1214 1542
Size: 1214 328
BanI G'GyrC_C
Cuts at: 0 1414 1542
Size: 1414 128
BanII G_rGCy'C
Cuts at: 0 1162 1542
Size: 1162 380
BbsI GAAGACnn'nnnn_
Cuts at: 0 554 1542
Size: 554 988
BbvCI CC'TCA_GC
Cuts at: 0 361 1270 1485 1542
Size: 361 909 215 57
Fragments arranged by size:
909 361 215 57
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 115 149 1542
Size: 115 34 1393
Fragments arranged by size:
1393 115 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 433 1542
Size: 433 1109
BclI T'GATC_A
Cuts at: 0 857 1542
Size: 857 685
BglI GCCn_nnn'nGGC
Cuts at: 0 1138 1542
Size: 1138 404
Bme1580I G_kGCm'C
Cuts at: 0 28 1542
Size: 28 1514
BmgBI CAC'GTC
Cuts at: 0 29 1542
Size: 29 1513
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 103 1542
Size: 103 1439
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 1542
Size: 462 1080
Bpu10I CC'TnA_GC
Cuts at: 0 361 1270 1485 1542
Size: 361 909 215 57
Fragments arranged by size:
909 361 215 57
BsaAI yAC'GTr
Cuts at: 0 36 1542
Size: 36 1506
BsaHI Gr'CG_yC
Cuts at: 0 1505 1542
Size: 1505 37
BsaWI w'CCGG_w
Cuts at: 0 1077 1542
Size: 1077 465
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 893 923 1450 1480 1542
Size: 893 30 527 30 62
Fragments arranged by size:
893 527 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 465 517 1542
Size: 465 52 1025
Fragments arranged by size:
1025 465 52
BseYI C'CCAG_C
Cuts at: 0 226 1542
Size: 226 1316
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1360 1542
Size: 1360 182
BsmBI CGTCTCn'nnnn_
Cuts at: 0 74 1542
Size: 74 1468
Bsp1286I G_dGCh'C
Cuts at: 0 28 1162 1542
Size: 28 1134 380
Fragments arranged by size:
1134 380 28
BspHI T'CATG_A
Cuts at: 0 756 1151 1542
Size: 756 395 391
Fragments arranged by size:
756 395 391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 937 1542
Size: 937 605
BsrDI GCAATG_nn'
Cuts at: 0 248 662 912 1082 1542
Size: 248 414 250 170 460
Fragments arranged by size:
460 414 250 248 170
BsrGI T'GTAC_A
Cuts at: 0 283 1040 1542
Size: 283 757 502
Fragments arranged by size:
757 502 283
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1005 1542
Size: 1005 537
BstXI CCAn_nnnn'nTGG
Cuts at: 0 243 898 1411 1542
Size: 243 655 513 131
Fragments arranged by size:
655 513 243 131
BstYI r'GATC_y
Cuts at: 0 1214 1542
Size: 1214 328
BtgI C'CryG_G
Cuts at: 0 206 1256 1542
Size: 206 1050 286
Fragments arranged by size:
1050 286 206
BtsI GCAGTG_nn'
Cuts at: 0 131 914 1043 1542
Size: 131 783 129 499
Fragments arranged by size:
783 499 131 129
DrdI GACnn_nn'nnGTC
Cuts at: 0 355 1542
Size: 355 1187
EaeI y'GGCC_r
Cuts at: 0 2 233 656 1542
Size: 2 231 423 886
Fragments arranged by size:
886 423 231 2
EarI CTCTTCn'nnn_
Cuts at: 0 73 521 828 1468 1542
Size: 73 448 307 640 74
Fragments arranged by size:
640 448 307 74 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 462 601 700 1542
Size: 462 139 99 842
Fragments arranged by size:
842 462 139 99
EcoO109I rG'GnC_Cy
Cuts at: 0 1139 1185 1542
Size: 1139 46 357
Fragments arranged by size:
1139 357 46
EcoRI G'AATT_C
Cuts at: 0 292 1542
Size: 292 1250
HaeII r_GCGC'y
Cuts at: 0 250 1068 1542
Size: 250 818 474
Fragments arranged by size:
818 474 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 144 176 1146 1178 1336 1368 1542
Size: 144 32 970 32 158 32 174
Fragments arranged by size:
970 174 158 144 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1416 1542
Size: 1416 126
MscI TGG'CCA
Cuts at: 0 235 658 1542
Size: 235 423 884
Fragments arranged by size:
884 423 235
MslI CAynn'nnrTG
Cuts at: 0 241 761 1150 1523 1542
Size: 241 520 389 373 19
Fragments arranged by size:
520 389 373 241 19
MspA1I CmG'CkG
Cuts at: 0 729 1542
Size: 729 813
NcoI C'CATG_G
Cuts at: 0 206 1256 1542
Size: 206 1050 286
Fragments arranged by size:
1050 286 206
PasI CC'CwG_GG
Cuts at: 0 115 828 1320 1542
Size: 115 713 492 222
Fragments arranged by size:
713 492 222 115
PflMI CCAn_nnn'nTGG
Cuts at: 0 1107 1542
Size: 1107 435
PshAI GACnn'nnGTC
Cuts at: 0 1432 1542
Size: 1432 110
PstI C_TGCA'G
Cuts at: 0 138 570 605 1542
Size: 138 432 35 937
Fragments arranged by size:
937 432 138 35
PvuII CAG'CTG
Cuts at: 0 729 1542
Size: 729 813
SapI GCTCTTCn'nnn_
Cuts at: 0 73 1542
Size: 73 1469
SbfI CC_TGCA'GG
Cuts at: 0 570 1542
Size: 570 972
SexAI A'CCwGG_T
Cuts at: 0 140 1542
Size: 140 1402
SfcI C'TryA_G
Cuts at: 0 134 566 601 1542
Size: 134 432 35 941
Fragments arranged by size:
941 432 134 35
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1138 1542
Size: 1138 404
SmlI C'TyrA_G
Cuts at: 0 181 1462 1542
Size: 181 1281 80
Fragments arranged by size:
1281 181 80
StyI C'CwwG_G
Cuts at: 0 206 368 398 1127 1134 1256 1542
Size: 206 162 30 729 7 122 286
Fragments arranged by size:
729 286 206 162 122 30 7
TatI w'GTAC_w
Cuts at: 0 283 863 1040 1448 1542
Size: 283 580 177 408 94
Fragments arranged by size:
580 408 283 177 94
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 278 310 893 925 1542
Size: 278 32 583 32 617
Fragments arranged by size:
617 583 278 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 214 1542
Size: 214 1328
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1152 1327 1498 1542
Size: 1152 175 171 44
Fragments arranged by size:
1152 175 171 44
Enzymes that do cut:
AarI AcuI AflII AhdI AlwNI ApoI BamHI BanI
BanII BbsI BbvCI BcgI BciVI BclI BglI Bme1580I
BmgBI BplI BpmI Bpu10I BsaAI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsmBI Bsp1286I BspHI BspMI BsrDI
BsrGI BstAPI BstXI BstYI BtgI BtsI DrdI EaeI
EarI Eco57MI EcoO109I EcoRI HaeII Hin4I MmeI MscI
MslI MspA1I NcoI PasI PflMI PshAI PstI PvuII
SapI SbfI SexAI SfcI SfiI SmlI StyI TatI
TstI Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BfrBI BglII BlpI BmrI BmtI
BpuEI BsaI BsaBI BsiEI BsiHKAI BsiWI BsmI BspEI
BsrBI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HincII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PfoI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI ScaI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII XbaI XhoI XmaI XmnI
ZraI