Restriction map for
pF1KB5030
(Linear) (Six-base) MAPSORT of: KIBB5030.seq Check: 1954 from: 1 to: 852
KIBB5030.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 341 852
Size: 341 511
AccI GT'mk_AC
Cuts at: 0 194 852
Size: 194 658
AflIII A'CryG_T
Cuts at: 0 140 852
Size: 140 712
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 852
Size: 193 659
ApoI r'AATT_y
Cuts at: 0 478 852
Size: 478 374
AvaI C'yCGr_G
Cuts at: 0 33 852
Size: 33 819
BanI G'GyrC_C
Cuts at: 0 169 466 852
Size: 169 297 386
Fragments arranged by size:
386 297 169
BbsI GAAGACnn'nnnn_
Cuts at: 0 596 852
Size: 596 256
BbvCI CC'TCA_GC
Cuts at: 0 719 852
Size: 719 133
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 39 73 852
Size: 39 34 779
Fragments arranged by size:
779 39 34
BfrBI ATG'CAT
Cuts at: 0 597 852
Size: 597 255
BmgBI CAC'GTC
Cuts at: 0 141 852
Size: 141 711
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 852
Size: 286 566
Bpu10I CC'TnA_GC
Cuts at: 0 719 852
Size: 719 133
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 852
Size: 565 287
BsaI GGTCTCn'nnnn_
Cuts at: 0 13 852
Size: 13 839
BsaAI yAC'GTr
Cuts at: 0 534 852
Size: 534 318
BsaBI GATnn'nnATC
Cuts at: 0 241 852
Size: 241 611
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 121 852
Size: 121 731
BseYI C'CCAG_C
Cuts at: 0 25 133 507 662 852
Size: 25 108 374 155 190
Fragments arranged by size:
374 190 155 108 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 852
Size: 556 296
BsiHKAI G_wGCw'C
Cuts at: 0 533 554 686 852
Size: 533 21 132 166
Fragments arranged by size:
533 166 132 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 780 852
Size: 780 72
Bsp1286I G_dGCh'C
Cuts at: 0 533 554 686 852
Size: 533 21 132 166
Fragments arranged by size:
533 166 132 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 852
Size: 341 511
BsrBI CCG'CTC
Cuts at: 0 440 852
Size: 440 412
BstXI CCAn_nnnn'nTGG
Cuts at: 0 411 639 852
Size: 411 228 213
Fragments arranged by size:
411 228 213
BstYI r'GATC_y
Cuts at: 0 408 852
Size: 408 444
BtgI C'CryG_G
Cuts at: 0 66 93 731 852
Size: 66 27 638 121
Fragments arranged by size:
638 121 66 27
BtsI GCAGTG_nn'
Cuts at: 0 684 852
Size: 684 168
DrdI GACnn_nn'nnGTC
Cuts at: 0 379 852
Size: 379 473
EaeI y'GGCC_r
Cuts at: 0 629 852
Size: 629 223
EarI CTCTTCn'nnn_
Cuts at: 0 738 852
Size: 738 114
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 852
Size: 19 833
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 852
Size: 286 566
EcoO109I rG'GnC_Cy
Cuts at: 0 603 723 852
Size: 603 120 129
Fragments arranged by size:
603 129 120
EcoRI G'AATT_C
Cuts at: 0 478 852
Size: 478 374
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 783 813 852
Size: 783 30 39
Fragments arranged by size:
783 39 30
HaeII r_GCGC'y
Cuts at: 0 215 852
Size: 215 637
MscI TGG'CCA
Cuts at: 0 631 852
Size: 631 221
MslI CAynn'nnrTG
Cuts at: 0 388 852
Size: 388 464
MspA1I CmG'CkG
Cuts at: 0 95 627 666 696 852
Size: 95 532 39 30 156
Fragments arranged by size:
532 156 95 39 30
NcoI C'CATG_G
Cuts at: 0 66 852
Size: 66 786
NsiI A_TGCA'T
Cuts at: 0 599 852
Size: 599 253
NspI r_CATG'y
Cuts at: 0 597 852
Size: 597 255
PflMI CCAn_nnn'nTGG
Cuts at: 0 696 699 852
Size: 696 3 153
Fragments arranged by size:
696 153 3
PfoI T'CCnGG_A
Cuts at: 0 263 852
Size: 263 589
PmlI CAC'GTG
Cuts at: 0 534 852
Size: 534 318
PpuMI rG'GwC_Cy
Cuts at: 0 603 852
Size: 603 249
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 46 78 852
Size: 46 32 774
Fragments arranged by size:
774 46 32
PstI C_TGCA'G
Cuts at: 0 185 852
Size: 185 667
PvuII CAG'CTG
Cuts at: 0 627 666 696 852
Size: 627 39 30 156
Fragments arranged by size:
627 156 39 30
SacII CC_GC'GG
Cuts at: 0 96 852
Size: 96 756
ScaI AGT'ACT
Cuts at: 0 55 852
Size: 55 797
SexAI A'CCwGG_T
Cuts at: 0 605 852
Size: 605 247
SfcI C'TryA_G
Cuts at: 0 181 852
Size: 181 671
SmaI CCC'GGG
Cuts at: 0 35 852
Size: 35 817
SmlI C'TyrA_G
Cuts at: 0 580 852
Size: 580 272
StyI C'CwwG_G
Cuts at: 0 66 377 404 852
Size: 66 311 27 448
Fragments arranged by size:
448 311 66 27
TatI w'GTAC_w
Cuts at: 0 53 852
Size: 53 799
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 411 852
Size: 411 441
XmaI C'CCGG_G
Cuts at: 0 33 852
Size: 33 819
Enzymes that do cut:
AarI AccI AflIII AlfI ApoI AvaI BanI BbsI
BbvCI BcgI BfrBI BmgBI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BstXI BstYI BtgI BtsI DrdI EaeI
EarI EciI Eco57MI EcoO109I EcoRI FalI HaeII MscI
MslI MspA1I NcoI NsiI NspI PflMI PfoI PmlI
PpuMI PsrI PstI PvuII SacII ScaI SexAI SfcI
SmaI SmlI StyI TatI XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BsaHI BsaWI
BsaXI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EagI EcoICRI EcoNI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PmeI
PpiI PshAI PsiI PspOMI PspXI PvuI RsrII SacI
SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TstI Tth111I XbaI XhoI XmnI ZraI