Restriction map for
pF1KB4945
(Linear) (Six-base) MAPSORT of: KIBB4945.seq Check: 9131 from: 1 to: 936
KIBB4945.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AccI GT'mk_AC
Cuts at: 0 404 781 936
Size: 404 377 155
Fragments arranged by size:
404 377 155
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 936
Size: 322 614
AflIII A'CryG_T
Cuts at: 0 329 936
Size: 329 607
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 250 282 936
Size: 250 32 654
Fragments arranged by size:
654 250 32
AlwNI CAG_nnn'CTG
Cuts at: 0 300 936
Size: 300 636
ApaI G_GGCC'C
Cuts at: 0 538 936
Size: 538 398
ApoI r'AATT_y
Cuts at: 0 171 936
Size: 171 765
BanI G'GyrC_C
Cuts at: 0 420 936
Size: 420 516
BanII G_rGCy'C
Cuts at: 0 185 538 936
Size: 185 353 398
Fragments arranged by size:
398 353 185
BbsI GAAGACnn'nnnn_
Cuts at: 0 778 936
Size: 778 158
Bme1580I G_kGCm'C
Cuts at: 0 538 697 936
Size: 538 159 239
Fragments arranged by size:
538 239 159
BmgBI CAC'GTC
Cuts at: 0 39 936
Size: 39 897
BmrI ACTGGGnnnn_n'
Cuts at: 0 707 936
Size: 707 229
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 886 936
Size: 169 717 50
Fragments arranged by size:
717 169 50
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 739 936
Size: 739 197
BsaAI yAC'GTr
Cuts at: 0 54 237 330 936
Size: 54 183 93 606
Fragments arranged by size:
606 183 93 54
BsaHI Gr'CG_yC
Cuts at: 0 680 936
Size: 680 256
BsaWI w'CCGG_w
Cuts at: 0 656 936
Size: 656 280
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 556 586 936
Size: 25 531 30 350
Fragments arranged by size:
531 350 30 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 22 630 633 936
Size: 19 3 608 3 303
Fragments arranged by size:
608 303 19 3 3
BseYI C'CCAG_C
Cuts at: 0 603 850 936
Size: 603 247 86
Fragments arranged by size:
603 247 86
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 313 508 901 936
Size: 313 195 393 35
Fragments arranged by size:
393 313 195 35
BsiHKAI G_wGCw'C
Cuts at: 0 23 185 242 936
Size: 23 162 57 694
Fragments arranged by size:
694 162 57 23
Bsp1286I G_dGCh'C
Cuts at: 0 23 185 242 538 697 936
Size: 23 162 57 296 159 239
Fragments arranged by size:
296 239 162 159 57 23
BspEI T'CCGG_A
Cuts at: 0 656 936
Size: 656 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 75 575 936
Size: 75 500 361
Fragments arranged by size:
500 361 75
BstXI CCAn_nnnn'nTGG
Cuts at: 0 282 723 864 936
Size: 282 441 141 72
Fragments arranged by size:
441 282 141 72
BstYI r'GATC_y
Cuts at: 0 482 936
Size: 482 454
Bsu36I CC'TnA_GG
Cuts at: 0 205 936
Size: 205 731
BtgI C'CryG_G
Cuts at: 0 45 275 545 638 926 936
Size: 45 230 270 93 288 10
Fragments arranged by size:
288 270 230 93 45 10
DrdI GACnn_nn'nnGTC
Cuts at: 0 547 936
Size: 547 389
EaeI y'GGCC_r
Cuts at: 0 68 272 474 936
Size: 68 204 202 462
Fragments arranged by size:
462 204 202 68
EcoICRI GAG'CTC
Cuts at: 0 183 936
Size: 183 753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 322 886 936
Size: 169 153 564 50
Fragments arranged by size:
564 169 153 50
EcoO109I rG'GnC_Cy
Cuts at: 0 390 535 702 903 936
Size: 390 145 167 201 33
Fragments arranged by size:
390 201 167 145 33
EcoRI G'AATT_C
Cuts at: 0 171 936
Size: 171 765
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 609 641 936
Size: 556 32 21 32 295
Fragments arranged by size:
556 295 32 32 21
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 587 936
Size: 587 349
MscI TGG'CCA
Cuts at: 0 274 936
Size: 274 662
MslI CAynn'nnrTG
Cuts at: 0 280 658 936
Size: 280 378 278
Fragments arranged by size:
378 280 278
MspA1I CmG'CkG
Cuts at: 0 47 81 631 864 936
Size: 47 34 550 233 72
Fragments arranged by size:
550 233 72 47 34
NcoI C'CATG_G
Cuts at: 0 275 936
Size: 275 661
NspI r_CATG'y
Cuts at: 0 522 936
Size: 522 414
PfoI T'CCnGG_A
Cuts at: 0 185 383 936
Size: 185 198 553
Fragments arranged by size:
553 198 185
PpuMI rG'GwC_Cy
Cuts at: 0 390 702 903 936
Size: 390 312 201 33
Fragments arranged by size:
390 312 201 33
PspOMI G'GGCC_C
Cuts at: 0 534 936
Size: 534 402
PstI C_TGCA'G
Cuts at: 0 86 803 936
Size: 86 717 133
Fragments arranged by size:
717 133 86
PvuII CAG'CTG
Cuts at: 0 864 936
Size: 864 72
SacI G_AGCT'C
Cuts at: 0 185 936
Size: 185 751
SacII CC_GC'GG
Cuts at: 0 48 936
Size: 48 888
SfcI C'TryA_G
Cuts at: 0 82 799 936
Size: 82 717 137
Fragments arranged by size:
717 137 82
SmlI C'TyrA_G
Cuts at: 0 754 936
Size: 754 182
StuI AGG'CCT
Cuts at: 0 203 936
Size: 203 733
StyI C'CwwG_G
Cuts at: 0 275 936
Size: 275 661
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 337 936
Size: 337 599
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 936
Size: 27 909
Tth111I GACn'n_nGTC
Cuts at: 0 682 777 936
Size: 682 95 159
Fragments arranged by size:
682 159 95
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApaI ApoI BanI
BanII BbsI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspEI BspMI BstXI BstYI Bsu36I BtgI DrdI EaeI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I MmeI MscI MslI
MspA1I NcoI NspI PfoI PpuMI PspOMI PstI PvuII
SacI SacII SfcI SmlI StuI StyI TaqII TstI
Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmtI BplI Bpu10I BsaI BsaBI
BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII EagI EarI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PflMI PmeI PmlI PpiI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI XbaI XcmI XhoI
XmaI XmnI ZraI