(Linear) (Six-base) MAPSORT of: KIBB4929.seq  Check: 2631  from: 1  to: 594



KIBB4929.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     594 
   Size:        437     157
 
AflIII A'CryG_T

Cuts at:      0     128     156     234     594 
   Size:        128      28      78     360

  Fragments arranged by size: 

                 360     128      78      28
 
AhdI GACnn_n'nnGTC

Cuts at:      0     157     594 
   Size:        157     437
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     506     594 
   Size:        506      88
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     326     594 
   Size:        326     268
 
BsiHKAI G_wGCw'C

Cuts at:      0     542     594 
   Size:        542      52
 
BsmI GAATG_Cn'

Cuts at:      0     510     594 
   Size:        510      84
 
Bsp1286I G_dGCh'C

Cuts at:      0     542     594 
   Size:        542      52
 
BsrDI GCAATG_nn'

Cuts at:      0     118     194     368     594 
   Size:        118      76     174     226

  Fragments arranged by size: 

                 226     174     118      76
 
BtsI GCAGTG_nn'

Cuts at:      0      45     594 
   Size:         45     549
 
EarI CTCTTCn'nnn_

Cuts at:      0     243     425     594 
   Size:        243     182     169

  Fragments arranged by size: 

                 243     182     169
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     437     594 
   Size:        437     157
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     358     594 
   Size:        358     236
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     143     175     594 
   Size:        143      32     419

  Fragments arranged by size: 

                 419     143      32
 
MspA1I CmG'CkG

Cuts at:      0     572     594 
   Size:        572      22
 
NspI r_CATG'y

Cuts at:      0     132     160     238     594 
   Size:        132      28      78     356

  Fragments arranged by size: 

                 356     132      78      28
 
PciI A'CATG_T

Cuts at:      0     128     156     234     594 
   Size:        128      28      78     360

  Fragments arranged by size: 

                 360     128      78      28
 
SfcI C'TryA_G

Cuts at:      0      46     594 
   Size:         46     548
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     217     594 
   Size:        217     377
 
TatI w'GTAC_w

Cuts at:      0     584     594 
   Size:        584      10
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      12      44     594 
   Size:         12      32     550

  Fragments arranged by size: 

                 550      32      12
 

 Enzymes that do cut:

     AcuI   AflIII     AhdI     BplI     BsgI  BsiHKAI     BsmI Bsp1286I
    BsrDI     BtsI     EarI  Eco57MI     FalI    Hin4I   MspA1I     NspI
     PciI     SfcI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI