Restriction map for
pF1KB4900
(Linear) (Six-base) MAPSORT of: KIBB4900.seq Check: 9033 from: 1 to: 1626
KIBB4900.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1525 1626
Size: 1525 101
AflIII A'CryG_T
Cuts at: 0 527 1626
Size: 527 1099
AlwNI CAG_nnn'CTG
Cuts at: 0 282 709 798 1626
Size: 282 427 89 828
Fragments arranged by size:
828 427 282 89
ApoI r'AATT_y
Cuts at: 0 1325 1626
Size: 1325 301
BanII G_rGCy'C
Cuts at: 0 26 1626
Size: 26 1600
BbsI GAAGACnn'nnnn_
Cuts at: 0 735 920 1626
Size: 735 185 706
Fragments arranged by size:
735 706 185
BclI T'GATC_A
Cuts at: 0 518 1626
Size: 518 1108
Bme1580I G_kGCm'C
Cuts at: 0 451 605 1626
Size: 451 154 1021
Fragments arranged by size:
1021 451 154
BmrI ACTGGGnnnn_n'
Cuts at: 0 691 1462 1626
Size: 691 771 164
Fragments arranged by size:
771 691 164
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 1626
Size: 288 1338
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1226 1626
Size: 1226 400
BsaWI w'CCGG_w
Cuts at: 0 1433 1626
Size: 1433 193
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1474 1504 1626
Size: 1474 30 122
Fragments arranged by size:
1474 122 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 700 994 1626
Size: 700 294 632
Fragments arranged by size:
700 632 294
BseYI C'CCAG_C
Cuts at: 0 892 1471 1626
Size: 892 579 155
Fragments arranged by size:
892 579 155
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1626
Size: 118 1508
BsiHKAI G_wGCw'C
Cuts at: 0 1172 1487 1626
Size: 1172 315 139
Fragments arranged by size:
1172 315 139
BsmI GAATG_Cn'
Cuts at: 0 147 365 1626
Size: 147 218 1261
Fragments arranged by size:
1261 218 147
Bsp1286I G_dGCh'C
Cuts at: 0 26 451 605 1172 1487 1626
Size: 26 425 154 567 315 139
Fragments arranged by size:
567 425 315 154 139 26
BspEI T'CCGG_A
Cuts at: 0 1433 1626
Size: 1433 193
BspHI T'CATG_A
Cuts at: 0 833 1626
Size: 833 793
BsrBI CCG'CTC
Cuts at: 0 1075 1626
Size: 1075 551
BsrDI GCAATG_nn'
Cuts at: 0 18 395 1235 1626
Size: 18 377 840 391
Fragments arranged by size:
840 391 377 18
BsrGI T'GTAC_A
Cuts at: 0 947 1626
Size: 947 679
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1194 1452 1626
Size: 1194 258 174
Fragments arranged by size:
1194 258 174
BstYI r'GATC_y
Cuts at: 0 706 1626
Size: 706 920
Bsu36I CC'TnA_GG
Cuts at: 0 1331 1626
Size: 1331 295
BtgI C'CryG_G
Cuts at: 0 659 869 1626
Size: 659 210 757
Fragments arranged by size:
757 659 210
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1389 1626
Size: 1389 237
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 378 413 1626
Size: 378 35 1213
Fragments arranged by size:
1213 378 35
DraI TTT'AAA
Cuts at: 0 561 570 597 1626
Size: 561 9 27 1029
Fragments arranged by size:
1029 561 27 9
DrdI GACnn_nn'nnGTC
Cuts at: 0 1153 1626
Size: 1153 473
EaeI y'GGCC_r
Cuts at: 0 83 1626
Size: 83 1543
EarI CTCTTCn'nnn_
Cuts at: 0 14 580 886 1092 1124 1538 1626
Size: 14 566 306 206 32 414 88
Fragments arranged by size:
566 414 306 206 88 32 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 1525 1626
Size: 288 1237 101
Fragments arranged by size:
1237 288 101
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1213 1626
Size: 1213 413
EcoO109I rG'GnC_Cy
Cuts at: 0 795 1082 1626
Size: 795 287 544
Fragments arranged by size:
795 544 287
EcoRI G'AATT_C
Cuts at: 0 1325 1626
Size: 1325 301
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 609 1626
Size: 609 1017
HaeII r_GCGC'y
Cuts at: 0 331 1626
Size: 331 1295
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 185 217 356 388 1154 1186 1474 1506
Size: 185 32 139 32 766 32 288 32
Cuts at: 1506 1626
Size: 120
Fragments arranged by size:
766 288 185 139 120 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1248 1626
Size: 1248 378
HindIII A'AGCT_T
Cuts at: 0 591 1626
Size: 591 1035
HpaI GTT'AAC
Cuts at: 0 1248 1626
Size: 1248 378
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1626
Size: 228 1398
MscI TGG'CCA
Cuts at: 0 85 1626
Size: 85 1541
MslI CAynn'nnrTG
Cuts at: 0 319 400 922 1192 1408 1626
Size: 319 81 522 270 216 218
Fragments arranged by size:
522 319 270 218 216 81
MspA1I CmG'CkG
Cuts at: 0 169 496 1303 1321 1626
Size: 169 327 807 18 305
Fragments arranged by size:
807 327 305 169 18
NcoI C'CATG_G
Cuts at: 0 659 1626
Size: 659 967
NspI r_CATG'y
Cuts at: 0 465 531 1626
Size: 465 66 1095
Fragments arranged by size:
1095 465 66
PasI CC'CwG_GG
Cuts at: 0 729 1464 1626
Size: 729 735 162
Fragments arranged by size:
735 729 162
PciI A'CATG_T
Cuts at: 0 527 1626
Size: 527 1099
PfoI T'CCnGG_A
Cuts at: 0 509 702 1421 1626
Size: 509 193 719 205
Fragments arranged by size:
719 509 205 193
PpuMI rG'GwC_Cy
Cuts at: 0 1082 1626
Size: 1082 544
PshAI GACnn'nnGTC
Cuts at: 0 1369 1626
Size: 1369 257
PstI C_TGCA'G
Cuts at: 0 501 1626
Size: 501 1125
PvuII CAG'CTG
Cuts at: 0 496 1303 1321 1626
Size: 496 807 18 305
Fragments arranged by size:
807 496 305 18
SapI GCTCTTCn'nnn_
Cuts at: 0 14 1626
Size: 14 1612
ScaI AGT'ACT
Cuts at: 0 238 1626
Size: 238 1388
SfcI C'TryA_G
Cuts at: 0 368 497 1626
Size: 368 129 1129
Fragments arranged by size:
1129 368 129
SmlI C'TyrA_G
Cuts at: 0 1241 1626
Size: 1241 385
StyI C'CwwG_G
Cuts at: 0 189 659 1033 1626
Size: 189 470 374 593
Fragments arranged by size:
593 470 374 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 619 910 1626
Size: 619 291 716
Fragments arranged by size:
716 619 291
TatI w'GTAC_w
Cuts at: 0 236 947 1626
Size: 236 711 679
Fragments arranged by size:
711 679 236
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 399 837 1191 1449 1626
Size: 399 438 354 258 177
Fragments arranged by size:
438 399 354 258 177
XmnI GAAnn'nnTTC
Cuts at: 0 529 1626
Size: 529 1097
Enzymes that do cut:
AcuI AflIII AlwNI ApoI BanII BbsI BclI Bme1580I
BmrI BpmI BpuEI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspHI BsrBI BsrDI BsrGI
BstXI BstYI Bsu36I BtgI BtgZI CspCI DraI DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NcoI NspI PasI PciI PfoI PpuMI PshAI PstI
PvuII SapI ScaI SfcI SmlI StyI TaqII TatI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmtI
BplI Bpu10I BsaI BsaAI BsaBI BsaHI BsiEI BsiWI
BsmBI BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtsI ClaI DraIII EagI EciI EcoICRI EcoRV
FseI FspI FspAI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PflMI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI
XmaI ZraI