Restriction map for
pF1KB4872
(Linear) (Six-base) MAPSORT of: KIBB4872.seq Check: 2601 from: 1 to: 8832
KIBB4872.orf
With 182 enzymes: SgfI *
May 11, 2012 13:46 ..
AatII G_ACGT'C
Cuts at: 0 4542 8832
Size: 4542 4290
AccI GT'mk_AC
Cuts at: 0 2245 5126 6459 8832
Size: 2245 2881 1333 2373
Fragments arranged by size:
2881 2373 2245 1333
Acc65I G'GTAC_C
Cuts at: 0 8532 8675 8832
Size: 8532 143 157
Fragments arranged by size:
8532 157 143
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 568 585 4580 6031 6509 6634 6686
Size: 508 60 17 3995 1451 478 125 52
Cuts at: 6686 8197 8618 8832
Size: 1511 421 214
Fragments arranged by size:
3995 1511 1451 508 478 421 214 125
60 52 17
AflIII A'CryG_T
Cuts at: 0 2886 3431 8832
Size: 2886 545 5401
Fragments arranged by size:
5401 2886 545
AgeI A'CCGG_T
Cuts at: 0 1331 3838 8832
Size: 1331 2507 4994
Fragments arranged by size:
4994 2507 1331
AhdI GACnn_n'nnGTC
Cuts at: 0 415 450 8832
Size: 415 35 8382
Fragments arranged by size:
8382 415 35
AleI CACnn'nnGTG
Cuts at: 0 3409 8832
Size: 3409 5423
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1636 3367 8832
Size: 1636 1731 5465
Fragments arranged by size:
5465 1731 1636
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 986 1018 3151 3183 5438 5470 8832
Size: 986 32 2133 32 2255 32 3362
Fragments arranged by size:
3362 2255 2133 986 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 361 462 825 1116 1260 1594 1926 2239
Size: 361 101 363 291 144 334 332 313
Cuts at: 2239 2482 4876 5137 6301 8317 8467 8821 8832
Size: 243 2394 261 1164 2016 150 354 11
Fragments arranged by size:
2394 2016 1164 363 361 354 334 332
313 291 261 243 150 144 101 11
ApaI G_GGCC'C
Cuts at: 0 1100 1123 1214 1282 2023 2062 2265 2288
Size: 1100 23 91 68 741 39 203 23
Cuts at: 2288 2741 2831 3863 4123 4405 4460 4682 4691
Size: 453 90 1032 260 282 55 222 9
Cuts at: 4691 4726 5195 5375 5689 5731 5965 5983 6076
Size: 35 469 180 314 42 234 18 93
Cuts at: 6076 6257 6891 7409 7490 8016 8832
Size: 181 634 518 81 526 816
Fragments arranged by size:
1100 1032 816 741 634 526 518 469
453 314 282 260 234 222 203 181
180 93 91 90 81 68 55 42
39 35 23 23 18 9
ApaLI G'TGCA_C
Cuts at: 0 218 645 8832
Size: 218 427 8187
Fragments arranged by size:
8187 427 218
AvaI C'yCGr_G
Cuts at: 0 56 1558 1730 2726 2740 3828 4008 4025
Size: 56 1502 172 996 14 1088 180 17
Cuts at: 4025 4514 4886 4941 5112 5186 6079 6124 6453
Size: 489 372 55 171 74 893 45 329
Cuts at: 6453 6570 7125 7215 7232 7409 7472 7628 7655
Size: 117 555 90 17 177 63 156 27
Cuts at: 7655 7791 7862 7954 8327 8792 8832
Size: 136 71 92 373 465 40
Fragments arranged by size:
1502 1088 996 893 555 489 465 373
372 329 180 177 172 171 156 136
117 92 90 74 71 63 56 55
45 40 27 17 17 14
AvrII C'CTAG_G
Cuts at: 0 2808 8832
Size: 2808 6024
BamHI G'GATC_C
Cuts at: 0 38 3359 4510 5755 7778 7795 8832
Size: 38 3321 1151 1245 2023 17 1037
Fragments arranged by size:
3321 2023 1245 1151 1037 38 17
BanI G'GyrC_C
Cuts at: 0 51 1054 1311 1597 2131 2453 2667 3438
Size: 51 1003 257 286 534 322 214 771
Cuts at: 3438 3455 3609 4983 5587 6565 6787 7597 8532
Size: 17 154 1374 604 978 222 810 935
Cuts at: 8532 8675 8832
Size: 143 157
Fragments arranged by size:
1374 1003 978 935 810 771 604 534
322 286 257 222 214 157 154 143
51 17
BanII G_rGCy'C
Cuts at: 0 69 1100 1123 1214 1282 1528 1588 2023
Size: 69 1031 23 91 68 246 60 435
Cuts at: 2023 2062 2265 2288 2741 2747 2831 3737 3863
Size: 39 203 23 453 6 84 906 126
Cuts at: 3863 3963 3979 4053 4123 4362 4405 4460 4682
Size: 100 16 74 70 239 43 55 222
Cuts at: 4682 4691 4726 5195 5223 5375 5637 5689 5731
Size: 9 35 469 28 152 262 52 42
Cuts at: 5731 5899 5965 5983 6076 6131 6257 6774 6820
Size: 168 66 18 93 55 126 517 46
Cuts at: 6820 6891 6914 6942 7049 7083 7132 7409 7490
Size: 71 23 28 107 34 49 277 81
Cuts at: 7490 7717 8016 8265 8283 8650 8832
Size: 227 299 249 18 367 182
Fragments arranged by size:
1031 906 517 469 453 435 367 299
277 262 249 246 239 227 222 203
182 168 152 126 126 107 100 93
91 84 81 74 71 70 69 68
66 60 55 55 52 49 46 43
42 39 35 34 28 28 23 23
23 18 18 16 9 6
BbeI G_GCGC'C
Cuts at: 0 55 2135 3459 3613 5591 8832
Size: 55 2080 1324 154 1978 3241
Fragments arranged by size:
3241 2080 1978 1324 154 55
BbsI GAAGACnn'nnnn_
Cuts at: 0 381 4273 5561 8832
Size: 381 3892 1288 3271
Fragments arranged by size:
3892 3271 1288 381
BbvCI CC'TCA_GC
Cuts at: 0 2647 8832
Size: 2647 6185
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 162 196 3583 3617 8832
Size: 162 34 3387 34 5215
Fragments arranged by size:
5215 3387 162 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2134 5653 7834 8832
Size: 2134 3519 2181 998
Fragments arranged by size:
3519 2181 2134 998
BclI T'GATC_A
Cuts at: 0 437 8832
Size: 437 8395
BglI GCCn_nnn'nGGC
Cuts at: 0 2209 2269 3149 3258 3830 3974 4313 4686
Size: 2209 60 880 109 572 144 339 373
Cuts at: 4686 4730 4799 5379 5459 5693 5925 5969 5987
Size: 44 69 580 80 234 232 44 18
Cuts at: 5987 6527 6704 7181 7457 8832
Size: 540 177 477 276 1375
Fragments arranged by size:
2209 1375 880 580 572 540 477 373
339 276 234 232 177 144 109 80
69 60 44 44 18
BglII A'GATC_T
Cuts at: 0 7167 8832
Size: 7167 1665
BlpI GC'TnA_GC
Cuts at: 0 328 1705 2213 2746 7716 8832
Size: 328 1377 508 533 4970 1116
Fragments arranged by size:
4970 1377 1116 533 508 328
Bme1580I G_kGCm'C
Cuts at: 0 222 649 1100 1123 1214 1282 1432 2023
Size: 222 427 451 23 91 68 150 591
Cuts at: 2023 2062 2265 2288 2560 2672 2741 2831 3145
Size: 39 203 23 272 112 69 90 314
Cuts at: 3145 3406 3863 4123 4405 4460 4682 4691 4726
Size: 261 457 260 282 55 222 9 35
Cuts at: 4726 4986 5195 5375 5689 5731 5921 5965 5983
Size: 260 209 180 314 42 190 44 18
Cuts at: 5983 6076 6257 6570 6891 7409 7490 8016 8832
Size: 93 181 313 321 518 81 526 816
Fragments arranged by size:
816 591 526 518 457 451 427 321
314 314 313 282 272 261 260 260
222 222 209 203 190 181 180 150
112 93 91 90 81 69 68 55
44 42 39 35 23 23 18 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 1582 1599 2056 2065 2178 3736 4153 6526
Size: 1582 17 457 9 113 1558 417 2373
Cuts at: 6526 8135 8832
Size: 1609 697
Fragments arranged by size:
2373 1609 1582 1558 697 457 417 113
17 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 1483 1602 1886 1911 2248 2420 2791
Size: 227 1256 119 284 25 337 172 371
Cuts at: 2791 2840 2915 3247 3599 3797 3923 3965 3977
Size: 49 75 332 352 198 126 42 12
Cuts at: 3977 4346 4439 4493 4565 4766 4793 4856 5009
Size: 369 93 54 72 201 27 63 153
Cuts at: 5009 5054 5159 5237 5570 5648 5801 6017 6332
Size: 45 105 78 333 78 153 216 315
Cuts at: 6332 6626 6647 6689 6713 6938 6965 6977 7067
Size: 294 21 42 24 225 27 12 90
Cuts at: 7067 7136 7409 7568 8297 8832
Size: 69 273 159 729 535
Fragments arranged by size:
1256 729 535 371 369 352 337 333
332 315 294 284 273 227 225 216
201 198 172 159 153 153 126 119
105 93 90 78 78 75 72 69
63 54 49 45 42 42 27 27
25 24 21 12 12
Bpu10I CC'TnA_GC
Cuts at: 0 2318 2647 6344 8832
Size: 2318 329 3697 2488
Fragments arranged by size:
3697 2488 2318 329
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 630 762 3012 3170 3525 6319 7855 8832
Size: 630 132 2250 158 355 2794 1536 977
Fragments arranged by size:
2794 2250 1536 977 630 355 158 132
BsaI GGTCTCn'nnnn_
Cuts at: 0 701 859 892 2503 4391 4742 4760 4769
Size: 701 158 33 1611 1888 351 18 9
Cuts at: 4769 4841 5012 5875 6020 6407 6542 6731 6953
Size: 72 171 863 145 387 135 189 222
Cuts at: 6953 7046 7376 8832
Size: 93 330 1456
Fragments arranged by size:
1888 1611 1456 863 701 387 351 330
222 189 171 158 145 135 93 72
33 18 9
BsaAI yAC'GTr
Cuts at: 0 2622 2887 3468 8832
Size: 2622 265 581 5364
Fragments arranged by size:
5364 2622 581 265
BsaHI Gr'CG_yC
Cuts at: 0 52 2132 3062 3085 3133 3456 3610 3915
Size: 52 2080 930 23 48 323 154 305
Cuts at: 3915 4539 5588 5928 8832
Size: 624 1049 340 2904
Fragments arranged by size:
2904 2080 1049 930 624 340 323 305
154 52 48 23
BsaWI w'CCGG_w
Cuts at: 0 681 1331 1908 3838 4702 8832
Size: 681 650 577 1930 864 4130
Fragments arranged by size:
4130 1930 864 681 650 577
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 663 684 693 714 3061 3091 3828 3858
Size: 663 21 9 21 2347 30 737 30
Cuts at: 3858 5438 5468 5589 5619 6624 6654 6807 6837
Size: 1580 30 121 30 1005 30 153 30
Cuts at: 6837 6950 6980 7106 7136 8237 8267 8429 8459
Size: 113 30 126 30 1101 30 162 30
Cuts at: 8459 8832
Size: 373
Fragments arranged by size:
2347 1580 1101 1005 737 663 373 162
153 126 121 113 30 30 30 30
30 30 30 30 30 30 21 21
9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 126 556 791 2029 3231 4709 5813 7250
Size: 126 430 235 1238 1202 1478 1104 1437
Cuts at: 7250 7844 8533 8596 8832
Size: 594 689 63 236
Fragments arranged by size:
1478 1437 1238 1202 1104 689 594 430
236 235 126 63
BseYI C'CCAG_C
Cuts at: 0 68 398 813 855 1116 1699 2657 3035
Size: 68 330 415 42 261 583 958 378
Cuts at: 3035 4503 4684 5292 5368 5404 6142 6481 8477
Size: 1468 181 608 76 36 738 339 1996
Cuts at: 8477 8832
Size: 355
Fragments arranged by size:
1996 1468 958 738 608 583 415 378
355 339 330 261 181 76 68 42
36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 75 265 2519 2647 2677 7058 7556 7790
Size: 75 190 2254 128 30 4381 498 234
Cuts at: 7790 8333 8637 8832
Size: 543 304 195
Fragments arranged by size:
4381 2254 543 498 304 234 195 190
128 75 30
BsiEI CG_ry'CG
Cuts at: 0 2906 4212 6021 8832
Size: 2906 1306 1809 2811
Fragments arranged by size:
2906 2811 1809 1306
BsiHKAI G_wGCw'C
Cuts at: 0 222 649 1189 1897 2642 2747 3846 4362
Size: 222 427 540 708 745 105 1099 516
Cuts at: 4362 5637 5899 7197 8283 8501 8832
Size: 1275 262 1298 1086 218 331
Fragments arranged by size:
1298 1275 1099 1086 745 708 540 516
427 331 262 222 218 105
BsmI GAATG_Cn'
Cuts at: 0 534 1675 8748 8832
Size: 534 1141 7073 84
Fragments arranged by size:
7073 1141 534 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3069 5414 8511 8832
Size: 3069 2345 3097 321
Fragments arranged by size:
3097 3069 2345 321
Bsp1286I G_dGCh'C
Cuts at: 0 69 222 649 1100 1123 1189 1214 1282
Size: 69 153 427 451 23 66 25 68
Cuts at: 1282 1432 1528 1588 1897 2023 2062 2265 2288
Size: 150 96 60 309 126 39 203 23
Cuts at: 2288 2560 2642 2672 2741 2747 2831 3145 3406
Size: 272 82 30 69 6 84 314 261
Cuts at: 3406 3737 3846 3863 3963 3979 4053 4123 4362
Size: 331 109 17 100 16 74 70 239
Cuts at: 4362 4405 4460 4682 4691 4726 4986 5195 5223
Size: 43 55 222 9 35 260 209 28
Cuts at: 5223 5375 5637 5689 5731 5899 5921 5965 5983
Size: 152 262 52 42 168 22 44 18
Cuts at: 5983 6076 6131 6257 6570 6774 6820 6891 6914
Size: 93 55 126 313 204 46 71 23
Cuts at: 6914 6942 7049 7083 7132 7197 7409 7490 7717
Size: 28 107 34 49 65 212 81 227
Cuts at: 7717 8016 8265 8283 8501 8650 8832
Size: 299 249 18 218 149 182
Fragments arranged by size:
451 427 331 314 313 309 299 272
262 261 260 249 239 227 222 218
212 209 204 203 182 168 153 152
150 149 126 126 109 107 100 96
93 84 82 81 74 71 70 69
69 68 66 65 60 55 55 52
49 46 44 43 42 39 35 34
30 28 28 25 23 23 23 22
18 18 17 16 9 6
BspEI T'CCGG_A
Cuts at: 0 681 1908 8832
Size: 681 1227 6924
Fragments arranged by size:
6924 1227 681
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 97 1547 4647 8832
Size: 97 1450 3100 4185
Fragments arranged by size:
4185 3100 1450 97
BsrBI CCG'CTC
Cuts at: 0 837 959 1644 3583 4221 5342 7269 8106
Size: 837 122 685 1939 638 1121 1927 837
Cuts at: 8106 8529 8832
Size: 423 303
Fragments arranged by size:
1939 1927 1121 837 837 685 638 423
303 122
BsrDI GCAATG_nn'
Cuts at: 0 4250 6369 8832
Size: 4250 2119 2463
Fragments arranged by size:
4250 2463 2119
BsrFI r'CCGG_y
Cuts at: 0 1331 2664 3838 8320 8470 8832
Size: 1331 1333 1174 4482 150 362
Fragments arranged by size:
4482 1333 1331 1174 362 150
BssHII G'CGCG_C
Cuts at: 0 2848 8832
Size: 2848 5984
BssSI C'ACGA_G
Cuts at: 0 704 1471 2864 3998 4133 8832
Size: 704 767 1393 1134 135 4699
Fragments arranged by size:
4699 1393 1134 767 704 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 217 1260 1569 3445 8467 8832
Size: 217 1043 309 1876 5022 365
Fragments arranged by size:
5022 1876 1043 365 309 217
BstEII G'GTnAC_C
Cuts at: 0 1176 4438 4936 5080 5308 5737 6367 6833
Size: 1176 3262 498 144 228 429 630 466
Cuts at: 6833 6988 7105 7786 8047 8083 8832
Size: 155 117 681 261 36 749
Fragments arranged by size:
3262 1176 749 681 630 498 466 429
261 228 155 144 117 36
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1414 2272 3340 3547 8832
Size: 1414 858 1068 207 5285
Fragments arranged by size:
5285 1414 1068 858 207
BstYI r'GATC_y
Cuts at: 0 38 2348 2489 3023 3359 4510 5028 5755
Size: 38 2310 141 534 336 1151 518 727
Cuts at: 5755 7167 7778 7795 8832
Size: 1412 611 17 1037
Fragments arranged by size:
2310 1412 1151 1037 727 611 534 518
336 141 38 17
BstZ17I GTA'TAC
Cuts at: 0 2246 8832
Size: 2246 6586
Bsu36I CC'TnA_GG
Cuts at: 0 539 1316 2591 2705 3113 6038 8540 8832
Size: 539 777 1275 114 408 2925 2502 292
Fragments arranged by size:
2925 2502 1275 777 539 408 292 114
BtgI C'CryG_G
Cuts at: 0 262 1323 1493 2166 2204 3147 3410 3644
Size: 262 1061 170 673 38 943 263 234
Cuts at: 3644 4161 4405 5085 5169 5439 5742 6021 6881
Size: 517 244 680 84 270 303 279 860
Cuts at: 6881 7591 8832
Size: 710 1241
Fragments arranged by size:
1241 1061 943 860 710 680 673 517
303 279 270 263 262 244 234 170
84 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 269 8409 8832
Size: 269 8140 423
Fragments arranged by size:
8140 423 269
BtsI GCAGTG_nn'
Cuts at: 0 823 974 1209 1758 1908 1924 3875 4634
Size: 823 151 235 549 150 16 1951 759
Cuts at: 4634 8190 8465 8654 8832
Size: 3556 275 189 178
Fragments arranged by size:
3556 1951 823 759 549 275 235 189
178 151 150 16
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 4715 4750 8832
Size: 4715 35 4082
Fragments arranged by size:
4715 4082 35
DraIII CAC_nnn'GTG
Cuts at: 0 226 1476 2625 2866 6565 7597 8832
Size: 226 1250 1149 241 3699 1032 1235
Fragments arranged by size:
3699 1250 1235 1149 1032 241 226
DrdI GACnn_nn'nnGTC
Cuts at: 0 2934 3673 5180 5281 6437 7114 7757 8832
Size: 2934 739 1507 101 1156 677 643 1075
Fragments arranged by size:
2934 1507 1156 1075 739 677 643 101
EaeI y'GGCC_r
Cuts at: 0 20 147 761 892 1197 1478 2712 2968
Size: 20 127 614 131 305 281 1234 256
Cuts at: 2968 2985 3686 3727 3930 4581 5334 5694 7032
Size: 17 701 41 203 651 753 360 1338
Cuts at: 7032 7449 7458 8308 8832
Size: 417 9 850 524
Fragments arranged by size:
1338 1234 850 753 701 651 614 524
417 360 305 281 256 203 131 127
41 20 17 9
EarI CTCTTCn'nnn_
Cuts at: 0 8519 8832
Size: 8519 313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1056 1762 2588 3172 4110 8832
Size: 1056 706 826 584 938 4722
Fragments arranged by size:
4722 1056 938 826 706 584
EcoICRI GAG'CTC
Cuts at: 0 2745 4360 5635 5897 8281 8832
Size: 2745 1615 1275 262 2384 551
Fragments arranged by size:
2745 2384 1615 1275 551 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 508 568 585 1483 1602 1886 1911
Size: 227 281 60 17 898 119 284 25
Cuts at: 1911 2248 2420 2791 2840 2915 3247 3599 3797
Size: 337 172 371 49 75 332 352 198
Cuts at: 3797 3923 3965 3977 4346 4439 4493 4565 4580
Size: 126 42 12 369 93 54 72 15
Cuts at: 4580 4766 4793 4856 5009 5054 5159 5237 5570
Size: 186 27 63 153 45 105 78 333
Cuts at: 5570 5648 5801 6017 6031 6332 6509 6626 6634
Size: 78 153 216 14 301 177 117 8
Cuts at: 6634 6647 6686 6689 6713 6938 6965 6977 7067
Size: 13 39 3 24 225 27 12 90
Cuts at: 7067 7136 7409 7568 8197 8297 8618 8832
Size: 69 273 159 629 100 321 214
Fragments arranged by size:
898 629 371 369 352 337 333 332
321 301 284 281 273 227 225 216
214 198 186 177 172 159 153 153
126 119 117 105 100 93 90 78
78 75 72 69 63 60 54 49
45 42 39 27 27 25 24 17
15 14 13 12 12 8 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 46 2118 3667 3700 4235 4465 7391 8832
Size: 46 2072 1549 33 535 230 2926 1441
Fragments arranged by size:
2926 2072 1549 1441 535 230 46 33
EcoO109I rG'GnC_Cy
Cuts at: 0 459 1083 1120 1210 1279 1738 1855 2019
Size: 459 624 37 90 69 459 117 164
Cuts at: 2019 2020 2261 2285 2730 2737 2827 2828 2980
Size: 1 241 24 445 7 90 1 152
Cuts at: 2980 3002 3109 3222 3859 3860 3950 4005 4021
Size: 22 107 113 637 1 90 55 16
Cuts at: 4021 4078 4104 4120 4149 4156 4225 4306 4401
Size: 57 26 16 29 7 69 81 95
Cuts at: 4401 4411 4456 4457 4555 4573 4620 4636 4678
Size: 10 45 1 98 18 47 16 42
Cuts at: 4678 4722 4783 4810 4828 4873 4927 4999 5098
Size: 44 61 27 18 45 54 72 99
Cuts at: 5098 5107 5134 5182 5191 5254 5274 5317 5372
Size: 9 27 48 9 63 20 43 55
Cuts at: 5372 5409 5451 5544 5659 5686 5728 5772 5935
Size: 37 42 93 115 27 42 44 163
Cuts at: 5935 5961 5962 5979 5980 6072 6073 6254 6298
Size: 26 1 17 1 92 1 181 44
Cuts at: 6298 6322 6376 6520 6592 6601 6637 6651 6887
Size: 24 54 144 72 9 36 14 236
Cuts at: 6887 6888 7087 7122 7228 7255 7282 7309 7355
Size: 1 199 35 106 27 27 27 46
Cuts at: 7355 7406 7468 7486 7487 7579 7624 7651 7671
Size: 51 62 18 1 92 45 27 20
Cuts at: 7671 7750 7858 7866 8012 8013 8028 8074 8137
Size: 79 108 8 146 1 15 46 63
Cuts at: 8137 8200 8236 8271 8314 8592 8759 8832
Size: 63 36 35 43 278 167 73
Fragments arranged by size:
637 624 459 459 445 278 241 236
199 181 167 164 163 152 146 144
117 115 113 108 107 106 99 98
95 93 92 92 90 90 90 81
79 73 72 72 69 69 63 63
63 62 61 57 55 55 54 54
51 48 47 46 46 45 45 45
44 44 44 43 43 42 42 42
37 37 36 36 35 35 29 27
27 27 27 27 27 27 26 26
24 24 22 20 20 18 18 18
17 16 16 16 15 14 10 9
9 9 8 7 7 1 1 1
1 1 1 1 1 1 1
FspI TGC'GCA
Cuts at: 0 1624 8832
Size: 1624 7208
HaeII r_GCGC'y
Cuts at: 0 55 2135 3459 3613 5591 8781 8832
Size: 55 2080 1324 154 1978 3190 51
Fragments arranged by size:
3190 2080 1978 1324 154 55 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 153 401 433 436 468 663 679
Size: 121 32 248 32 3 32 195 16
Cuts at: 679 684 695 711 716 2481 2513 3828 3860
Size: 5 11 16 5 1765 32 1315 32
Cuts at: 3860 4049 4081 5589 5621 5740 5772 5881 5913
Size: 189 32 1508 32 119 32 109 32
Cuts at: 5913 6624 6656 7106 7138 7215 7247 7286 7318
Size: 711 32 450 32 77 32 39 32
Cuts at: 7318 7699 7731 7780 7812 8539 8571 8632 8664
Size: 381 32 49 32 727 32 61 32
Cuts at: 8664 8832
Size: 168
Fragments arranged by size:
1765 1508 1315 727 711 450 381 248
195 189 168 121 119 109 77 61
49 39 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 16 16 11 5 5
3
HincII GTy'rAC
Cuts at: 0 7762 8832
Size: 7762 1070
KasI G'GCGC_C
Cuts at: 0 51 2131 3455 3609 5587 8832
Size: 51 2080 1324 154 1978 3245
Fragments arranged by size:
3245 2080 1978 1324 154 51
KpnI G_GTAC'C
Cuts at: 0 8536 8679 8832
Size: 8536 143 153
Fragments arranged by size:
8536 153 143
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1316 1391 4887 6837 8832
Size: 1316 75 3496 1950 1995
Fragments arranged by size:
3496 1995 1950 1316 75
MscI TGG'CCA
Cuts at: 0 1480 2714 2987 3688 5696 8832
Size: 1480 1234 273 701 2008 3136
Fragments arranged by size:
3136 2008 1480 1234 701 273
MslI CAynn'nnrTG
Cuts at: 0 1618 3409 8832
Size: 1618 1791 5423
Fragments arranged by size:
5423 1791 1618
MspA1I CmG'CkG
Cuts at: 0 109 402 475 760 859 1566 2818 3039
Size: 109 293 73 285 99 707 1252 221
Cuts at: 3039 3597 4507 4684 5171 5368 7567 8783 8832
Size: 558 910 177 487 197 2199 1216 49
Fragments arranged by size:
2199 1252 1216 910 707 558 487 293
285 221 197 177 109 99 73 49
NaeI GCC'GGC
Cuts at: 0 8472 8832
Size: 8472 360
NarI GG'CG_CC
Cuts at: 0 52 2132 3456 3610 5588 8832
Size: 52 2080 1324 154 1978 3244
Fragments arranged by size:
3244 2080 1978 1324 154 52
NgoMIV G'CCGG_C
Cuts at: 0 8470 8832
Size: 8470 362
NspI r_CATG'y
Cuts at: 0 2257 3181 3372 3435 8832
Size: 2257 924 191 63 5397
Fragments arranged by size:
5397 2257 924 191 63
PasI CC'CwG_GG
Cuts at: 0 1123 1632 2607 3855 4171 4461 4615 4852
Size: 1123 509 975 1248 316 290 154 237
Cuts at: 4852 5224 5323 5413 5655 5674 6250 7057 7135
Size: 372 99 90 242 19 576 807 78
Cuts at: 7135 7288 7314 7755 8170 8832
Size: 153 26 441 415 662
Fragments arranged by size:
1248 1123 975 807 662 576 509 441
415 372 316 290 242 237 154 153
99 90 78 26 19
PciI A'CATG_T
Cuts at: 0 3431 8832
Size: 3431 5401
PflMI CCAn_nnn'nTGG
Cuts at: 0 462 1476 1594 2114 3325 6301 6538 8122
Size: 462 1014 118 520 1211 2976 237 1584
Cuts at: 8122 8832
Size: 710
Fragments arranged by size:
2976 1584 1211 1014 710 520 462 237
118
PfoI T'CCnGG_A
Cuts at: 0 634 1300 1579 3092 3224 4053 4179 4284
Size: 634 666 279 1513 132 829 126 105
Cuts at: 4284 4416 4542 4743 4833 5031 5994 6231 6300
Size: 132 126 201 90 198 963 237 69
Cuts at: 6300 6642 6684 7083 8832
Size: 342 42 399 1749
Fragments arranged by size:
1749 1513 963 829 666 634 399 342
279 237 201 198 132 132 126 126
105 90 69 42
PmlI CAC'GTG
Cuts at: 0 2622 8832
Size: 2622 6210
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 5438 5470 8832
Size: 5438 32 3362
Fragments arranged by size:
5438 3362 32
PpuMI rG'GwC_Cy
Cuts at: 0 459 1083 1738 1855 2730 3002 3109 3222
Size: 459 624 655 117 875 272 107 113
Cuts at: 3222 3950 4005 4021 4078 4104 4149 4156 4225
Size: 728 55 16 57 26 45 7 69
Cuts at: 4225 4411 4555 4573 4620 4636 4783 4810 4873
Size: 186 144 18 47 16 147 27 63
Cuts at: 4873 4927 5098 5107 5134 5182 5254 5274 5317
Size: 54 171 9 27 48 72 20 43
Cuts at: 5317 5409 5544 6298 6322 6376 6592 6601 6637
Size: 92 135 754 24 54 216 9 36
Cuts at: 6637 7087 7122 7309 7468 7651 7671 7750 7858
Size: 450 35 187 159 183 20 79 108
Cuts at: 7858 8236 8271 8592 8759 8832
Size: 378 35 321 167 73
Fragments arranged by size:
875 754 728 655 624 459 450 378
321 272 216 187 186 183 171 167
159 147 144 135 117 113 108 107
92 79 73 72 69 63 57 55
54 54 48 47 45 43 36 35
35 27 27 26 24 20 20 18
16 16 9 9 7
PshAI GACnn'nnGTC
Cuts at: 0 3088 4781 4925 5315 6374 8832
Size: 3088 1693 144 390 1059 2458
Fragments arranged by size:
3088 2458 1693 1059 390 144
PspOMI G'GGCC_C
Cuts at: 0 1096 1119 1210 1278 2019 2058 2261 2284
Size: 1096 23 91 68 741 39 203 23
Cuts at: 2284 2737 2827 3859 4119 4401 4456 4678 4687
Size: 453 90 1032 260 282 55 222 9
Cuts at: 4687 4722 5191 5371 5685 5727 5961 5979 6072
Size: 35 469 180 314 42 234 18 93
Cuts at: 6072 6253 6887 7405 7486 8012 8832
Size: 181 634 518 81 526 820
Fragments arranged by size:
1096 1032 820 741 634 526 518 469
453 314 282 260 234 222 203 181
180 93 91 90 81 68 55 42
39 35 23 23 18 9
PspXI vC'TCGA_Gb
Cuts at: 0 1730 4025 5186 7232 8327 8832
Size: 1730 2295 1161 2046 1095 505
Fragments arranged by size:
2295 2046 1730 1161 1095 505
PstI C_TGCA'G
Cuts at: 0 1424 1967 2031 2348 2487 2838 4629 5397
Size: 1424 543 64 317 139 351 1791 768
Cuts at: 5397 6509 6591 8463 8832
Size: 1112 82 1872 369
Fragments arranged by size:
1872 1791 1424 1112 768 543 369 351
317 139 82 64
PvuI CG_AT'CG
Cuts at: 0 2906 4212 8832
Size: 2906 1306 4620
Fragments arranged by size:
4620 2906 1306
PvuII CAG'CTG
Cuts at: 0 402 475 859 2818 3597 7567 8832
Size: 402 73 384 1959 779 3970 1265
Fragments arranged by size:
3970 1959 1265 779 402 384 73
SacI G_AGCT'C
Cuts at: 0 2747 4362 5637 5899 8283 8832
Size: 2747 1615 1275 262 2384 549
Fragments arranged by size:
2747 2384 1615 1275 549 262
SacII CC_GC'GG
Cuts at: 0 5172 8832
Size: 5172 3660
SanDI GG'GwC_CC
Cuts at: 0 1738 1855 3002 3950 4005 4104 4149 4225
Size: 1738 117 1147 948 55 99 45 76
Cuts at: 4225 4620 4783 5098 5254 5274 5317 5409 5544
Size: 395 163 315 156 20 43 92 135
Cuts at: 5544 6322 7122 7468 7671 7750 7858 8271 8759
Size: 778 800 346 203 79 108 413 488
Cuts at: 8759 8832
Size: 73
Fragments arranged by size:
1738 1147 948 800 778 488 413 395
346 315 203 163 156 135 117 108
99 92 79 76 73 55 45 43
20
SapI GCTCTTCn'nnn_
Cuts at: 0 8519 8832
Size: 8519 313
SbfI CC_TGCA'GG
Cuts at: 0 6591 8832
Size: 6591 2241
ScaI AGT'ACT
Cuts at: 0 8554 8832
Size: 8554 278
SexAI A'CCwGG_T
Cuts at: 0 411 461 710 1982 4236 6048 6561 6837
Size: 411 50 249 1272 2254 1812 513 276
Cuts at: 6837 6962 7764 8832
Size: 125 802 1068
Fragments arranged by size:
2254 1812 1272 1068 802 513 411 276
249 125 50
SfcI C'TryA_G
Cuts at: 0 1420 1963 2027 2344 2483 2834 4625 5393
Size: 1420 543 64 317 139 351 1791 768
Cuts at: 5393 6505 6587 8459 8832
Size: 1112 82 1872 373
Fragments arranged by size:
1872 1791 1420 1112 768 543 373 351
317 139 82 64
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3258 4313 4686 5379 5459 5693 6527 6704
Size: 3258 1055 373 693 80 234 834 177
Cuts at: 6704 7181 7457 8832
Size: 477 276 1375
Fragments arranged by size:
3258 1375 1055 834 693 477 373 276
234 177 80
SfoI GGC'GCC
Cuts at: 0 53 2133 3457 3611 5589 8832
Size: 53 2080 1324 154 1978 3243
Fragments arranged by size:
3243 2080 1978 1324 154 53
SmaI CCC'GGG
Cuts at: 0 1560 2728 3830 4010 4516 4943 5114 6081
Size: 1560 1168 1102 180 506 427 171 967
Cuts at: 6081 6126 6455 6572 7127 7217 7793 7864 7956
Size: 45 329 117 555 90 576 71 92
Cuts at: 7956 8794 8832
Size: 838 38
Fragments arranged by size:
1560 1168 1102 967 838 576 555 506
427 329 180 171 117 92 90 71
45 38
SmlI C'TyrA_G
Cuts at: 0 609 741 1730 3027 3185 3504 4025 5186
Size: 609 132 989 1297 158 319 521 1161
Cuts at: 5186 6334 7232 7870 8327 8832
Size: 1148 898 638 457 505
Fragments arranged by size:
1297 1161 1148 989 898 638 609 521
505 457 319 158 132
SrfI GCCC'GGGC
Cuts at: 0 1560 8832
Size: 1560 7272
StuI AGG'CCT
Cuts at: 0 4367 5435 5669 6707 6950 8707 8770 8832
Size: 4367 1068 234 1038 243 1757 63 62
Fragments arranged by size:
4367 1757 1068 1038 243 234 63 62
StyI C'CwwG_G
Cuts at: 0 422 2808 3339 3881 4302 4550 4587 4710
Size: 422 2386 531 542 421 248 37 123
Cuts at: 4710 4760 5075 5195 5618 5646 5732 5939 6060
Size: 50 315 120 423 28 86 207 121
Cuts at: 6060 6275 6372 6597 7601 8042 8078 8605 8832
Size: 215 97 225 1004 441 36 527 227
Fragments arranged by size:
2386 1004 542 531 527 441 423 422
421 315 248 227 225 215 207 123
121 120 97 86 50 37 36 28
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 916 1618 1645 4453 4996 6447 6526 6958
Size: 916 702 27 2808 543 1451 79 432
Cuts at: 6958 7007 7603 8026 8066 8832
Size: 49 596 423 40 766
Fragments arranged by size:
2808 1451 916 766 702 596 543 432
423 79 49 40 27
TatI w'GTAC_w
Cuts at: 0 248 747 794 8552 8832
Size: 248 499 47 7758 280
Fragments arranged by size:
7758 499 280 248 47
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 6807 6839 6887 6919 6950 6982 7770 7802
Size: 6807 32 48 32 31 32 788 32
Cuts at: 7802 8237 8269 8429 8461 8832
Size: 435 32 160 32 371
Fragments arranged by size:
6807 788 435 371 160 48 32 32
32 32 32 32 31
Tth111I GACn'n_nGTC
Cuts at: 0 385 3625 8832
Size: 385 3240 5207
Fragments arranged by size:
5207 3240 385
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1224 1983 2191 2995 3676 3862 4309 5029
Size: 1224 759 208 804 681 186 447 720
Cuts at: 5029 5704 6282 6604 7608 7762 8832
Size: 675 578 322 1004 154 1070
Fragments arranged by size:
1224 1070 1004 804 759 720 681 675
578 447 322 208 186 154
XhoI C'TCGA_G
Cuts at: 0 1730 4025 5186 7232 8327 8832
Size: 1730 2295 1161 2046 1095 505
Fragments arranged by size:
2295 2046 1730 1161 1095 505
XmaI C'CCGG_G
Cuts at: 0 1558 2726 3828 4008 4514 4941 5112 6079
Size: 1558 1168 1102 180 506 427 171 967
Cuts at: 6079 6124 6453 6570 7125 7215 7791 7862 7954
Size: 45 329 117 555 90 576 71 92
Cuts at: 7954 8792 8832
Size: 838 40
Fragments arranged by size:
1558 1168 1102 967 838 576 555 506
427 329 180 171 117 92 90 71
45 40
XmnI GAAnn'nnTTC
Cuts at: 0 5246 8210 8832
Size: 5246 2964 622
Fragments arranged by size:
5246 2964 622
ZraI GAC'GTC
Cuts at: 0 4540 8832
Size: 4540 4292
Enzymes that do cut:
AatII AccI Acc65I AcuI AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaI ApaLI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI
HaeII Hin4I HincII KasI KpnI MmeI MscI MslI
MspA1I NaeI NarI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII SacI SacII SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SmaI SmlI SrfI StuI
StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII ApoI AscI AseI AsiSI
BaeI BfrBI BmgBI BmtI BplI BsaBI BsiWI BspHI
BsrGI BstBI ClaI DraI EagI EcoRI EcoRV FalI
FseI FspAI HindIII HpaI MfeI MluI NcoI NdeI
NheI NotI NruI NsiI PacI PmeI PsiI PsrI
RsrII SalI SgfI SgrAI SnaBI SpeI SphI SspI
SwaI XbaI