Restriction map for
pF1KB4832
(Linear) (Six-base) MAPSORT of: KIBB4832.seq Check: 3360 from: 1 to: 1266
KIBB4832.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 173 632 1266
Size: 173 459 634
Fragments arranged by size:
634 459 173
AccI GT'mk_AC
Cuts at: 0 173 1266
Size: 173 1093
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 565 867 1266
Size: 565 302 399
Fragments arranged by size:
565 399 302
AlwNI CAG_nnn'CTG
Cuts at: 0 401 1173 1245 1266
Size: 401 772 72 21
Fragments arranged by size:
772 401 72 21
ApoI r'AATT_y
Cuts at: 0 598 1266
Size: 598 668
AseI AT'TA_AT
Cuts at: 0 770 1266
Size: 770 496
AvaI C'yCGr_G
Cuts at: 0 1186 1266
Size: 1186 80
BamHI G'GATC_C
Cuts at: 0 950 1266
Size: 950 316
BanI G'GyrC_C
Cuts at: 0 481 503 1266
Size: 481 22 763
Fragments arranged by size:
763 481 22
BanII G_rGCy'C
Cuts at: 0 26 706 1253 1266
Size: 26 680 547 13
Fragments arranged by size:
680 547 26 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 448 563 1079 1266
Size: 448 115 516 187
Fragments arranged by size:
516 448 187 115
BglI GCCn_nnn'nGGC
Cuts at: 0 665 1266
Size: 665 601
BglII A'GATC_T
Cuts at: 0 491 640 1266
Size: 491 149 626
Fragments arranged by size:
626 491 149
Bme1580I G_kGCm'C
Cuts at: 0 484 508 1237 1266
Size: 484 24 729 29
Fragments arranged by size:
729 484 29 24
BmgBI CAC'GTC
Cuts at: 0 963 1266
Size: 963 303
BmrI ACTGGGnnnn_n'
Cuts at: 0 709 1266
Size: 709 557
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 537 1266
Size: 537 729
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 769 1266
Size: 507 262 497
Fragments arranged by size:
507 497 262
BsaHI Gr'CG_yC
Cuts at: 0 170 629 1266
Size: 170 459 637
Fragments arranged by size:
637 459 170
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 281 311 1266
Size: 281 30 955
Fragments arranged by size:
955 281 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 547 858 1266
Size: 547 311 408
Fragments arranged by size:
547 408 311
BseYI C'CCAG_C
Cuts at: 0 409 716 1110 1149 1266
Size: 409 307 394 39 117
Fragments arranged by size:
409 394 307 117 39
BsiEI CG_ry'CG
Cuts at: 0 450 1266
Size: 450 816
BsiHKAI G_wGCw'C
Cuts at: 0 388 1264 1266
Size: 388 876 2
Fragments arranged by size:
876 388 2
BsmI GAATG_Cn'
Cuts at: 0 368 1266
Size: 368 898
BsmBI CGTCTCn'nnnn_
Cuts at: 0 899 1266
Size: 899 367
Bsp1286I G_dGCh'C
Cuts at: 0 26 388 484 508 706 1237 1253 1264
Size: 26 362 96 24 198 531 16 11
Cuts at: 1264 1266
Size: 2
Fragments arranged by size:
531 362 198 96 26 24 16 11
2
BsrDI GCAATG_nn'
Cuts at: 0 624 1266
Size: 624 642
BsrFI r'CCGG_y
Cuts at: 0 98 445 1266
Size: 98 347 821
Fragments arranged by size:
821 347 98
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1173 1266
Size: 1173 93
BstEII G'GTnAC_C
Cuts at: 0 160 309 1266
Size: 160 149 957
Fragments arranged by size:
957 160 149
BstYI r'GATC_y
Cuts at: 0 237 251 491 640 950 1225 1266
Size: 237 14 240 149 310 275 41
Fragments arranged by size:
310 275 240 237 149 41 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 51 1266
Size: 51 1215
EaeI y'GGCC_r
Cuts at: 0 447 473 1266
Size: 447 26 793
Fragments arranged by size:
793 447 26
EagI C'GGCC_G
Cuts at: 0 447 1266
Size: 447 819
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 507 565 769 867 1266
Size: 507 58 204 98 399
Fragments arranged by size:
507 399 204 98 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 1266
Size: 547 719
EcoO109I rG'GnC_Cy
Cuts at: 0 1065 1266
Size: 1065 201
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 281 313 1087 1119 1266
Size: 281 32 774 32 147
Fragments arranged by size:
774 281 147 32 32
HincII GTy'rAC
Cuts at: 0 174 1266
Size: 174 1092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 491 720 1266
Size: 491 229 546
Fragments arranged by size:
546 491 229
MscI TGG'CCA
Cuts at: 0 475 1266
Size: 475 791
MslI CAynn'nnrTG
Cuts at: 0 934 1266
Size: 934 332
MspA1I CmG'CkG
Cuts at: 0 249 339 427 452 716 1266
Size: 249 90 88 25 264 550
Fragments arranged by size:
550 264 249 90 88 25
PasI CC'CwG_GG
Cuts at: 0 285 1266
Size: 285 981
PfoI T'CCnGG_A
Cuts at: 0 734 1244 1266
Size: 734 510 22
Fragments arranged by size:
734 510 22
PstI C_TGCA'G
Cuts at: 0 110 188 1178 1266
Size: 110 78 990 88
Fragments arranged by size:
990 110 88 78
PvuII CAG'CTG
Cuts at: 0 427 1266
Size: 427 839
SalI G'TCGA_C
Cuts at: 0 172 1266
Size: 172 1094
SexAI A'CCwGG_T
Cuts at: 0 128 998 1266
Size: 128 870 268
Fragments arranged by size:
870 268 128
SfcI C'TryA_G
Cuts at: 0 106 184 1174 1266
Size: 106 78 990 92
Fragments arranged by size:
990 106 92 78
SspI AAT'ATT
Cuts at: 0 774 1266
Size: 774 492
StuI AGG'CCT
Cuts at: 0 112 1266
Size: 112 1154
StyI C'CwwG_G
Cuts at: 0 389 1266
Size: 389 877
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 67 129 1266
Size: 67 62 1137
Fragments arranged by size:
1137 67 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 422 454 1266
Size: 422 32 812
Fragments arranged by size:
812 422 32
Tth111I GACn'n_nGTC
Cuts at: 0 271 1266
Size: 271 995
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 696 1266
Size: 696 570
XmnI GAAnn'nnTTC
Cuts at: 0 368 1266
Size: 368 898
ZraI GAC'GTC
Cuts at: 0 171 630 1266
Size: 171 459 636
Fragments arranged by size:
636 459 171
Enzymes that do cut:
AatII AccI AcuI AlwNI ApoI AseI AvaI BamHI
BanI BanII BbsI BglI BglII Bme1580I BmgBI BmrI
BplI BpmI BsaHI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BsrDI BsrFI BstAPI BstEII BstYI
BtgZI EaeI EagI Eco57MI EcoNI EcoO109I Hin4I HincII
MmeI MscI MslI MspA1I PasI PfoI PstI PvuII
SalI SexAI SfcI SspI StuI StyI TaqII TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BbeI BbvCI BcgI BciVI BclI BfrBI BlpI
BmtI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiWI BspEI BspHI BspMI BsrBI BsrGI BssHII BssSI
BstBI BstXI BstZ17I Bsu36I BtgI BtsI ClaI CspCI
DraI DraIII DrdI EarI EciI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SwaI TatI XbaI XhoI XmaI