Restriction map for
pF1KB4828
(Linear) (Six-base) MAPSORT of: KIBB4828.seq Check: 7495 from: 1 to: 873
KIBB4828.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AatII G_ACGT'C
Cuts at: 0 445 873
Size: 445 428
AccI GT'mk_AC
Cuts at: 0 62 873
Size: 62 811
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 863 873
Size: 427 436 10
Fragments arranged by size:
436 427 10
AflIII A'CryG_T
Cuts at: 0 499 873
Size: 499 374
AleI CACnn'nnGTG
Cuts at: 0 502 873
Size: 502 371
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 28 873
Size: 28 845
AlwNI CAG_nnn'CTG
Cuts at: 0 245 841 873
Size: 245 596 32
Fragments arranged by size:
596 245 32
ApaLI G'TGCA_C
Cuts at: 0 316 873
Size: 316 557
BanII G_rGCy'C
Cuts at: 0 274 352 374 773 873
Size: 274 78 22 399 100
Fragments arranged by size:
399 274 100 78 22
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 851 873
Size: 115 736 22
Fragments arranged by size:
736 115 22
BbvCI CC'TCA_GC
Cuts at: 0 542 873
Size: 542 331
BclI T'GATC_A
Cuts at: 0 278 873
Size: 278 595
BglII A'GATC_T
Cuts at: 0 20 873
Size: 20 853
Bme1580I G_kGCm'C
Cuts at: 0 320 873
Size: 320 553
BmgBI CAC'GTC
Cuts at: 0 486 870 873
Size: 486 384 3
Fragments arranged by size:
486 384 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 873
Size: 872 1
Bpu10I CC'TnA_GC
Cuts at: 0 542 873
Size: 542 331
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 873
Size: 670 203
BsaI GGTCTCn'nnnn_
Cuts at: 0 391 873
Size: 391 482
BsaHI Gr'CG_yC
Cuts at: 0 442 873
Size: 442 431
BsaWI w'CCGG_w
Cuts at: 0 186 625 873
Size: 186 439 248
Fragments arranged by size:
439 248 186
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 559 873
Size: 559 314
BseYI C'CCAG_C
Cuts at: 0 154 873
Size: 154 719
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 279 873
Size: 279 594
BsiHKAI G_wGCw'C
Cuts at: 0 320 352 374 472 527 873
Size: 320 32 22 98 55 346
Fragments arranged by size:
346 320 98 55 32 22
Bsp1286I G_dGCh'C
Cuts at: 0 274 320 352 374 472 527 773 873
Size: 274 46 32 22 98 55 246 100
Fragments arranged by size:
274 246 100 98 55 46 32 22
BspHI T'CATG_A
Cuts at: 0 125 873
Size: 125 748
BsrDI GCAATG_nn'
Cuts at: 0 488 873
Size: 488 385
BstAPI GCAn_nnn'nTGC
Cuts at: 0 245 873
Size: 245 628
BstYI r'GATC_y
Cuts at: 0 20 242 873
Size: 20 222 631
Fragments arranged by size:
631 222 20
BtsI GCAGTG_nn'
Cuts at: 0 10 291 343 873
Size: 10 281 52 530
Fragments arranged by size:
530 281 52 10
DraIII CAC_nnn'GTG
Cuts at: 0 315 873
Size: 315 558
EaeI y'GGCC_r
Cuts at: 0 611 873
Size: 611 262
EarI CTCTTCn'nnn_
Cuts at: 0 673 828 873
Size: 673 155 45
Fragments arranged by size:
673 155 45
EcoICRI GAG'CTC
Cuts at: 0 350 372 873
Size: 350 22 501
Fragments arranged by size:
501 350 22
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 863 872 873
Size: 427 436 9 1
Fragments arranged by size:
436 427 9 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 34 66 785 805 817 837 845 873
Size: 34 32 719 20 12 20 8 28
Fragments arranged by size:
719 34 32 28 20 20 12 8
MfeI C'AATT_G
Cuts at: 0 670 873
Size: 670 203
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 836 873
Size: 377 459 37
Fragments arranged by size:
459 377 37
MscI TGG'CCA
Cuts at: 0 613 873
Size: 613 260
MslI CAynn'nnrTG
Cuts at: 0 502 794 873
Size: 502 292 79
Fragments arranged by size:
502 292 79
NspI r_CATG'y
Cuts at: 0 9 503 873
Size: 9 494 370
Fragments arranged by size:
494 370 9
PciI A'CATG_T
Cuts at: 0 499 873
Size: 499 374
PstI C_TGCA'G
Cuts at: 0 241 335 873
Size: 241 94 538
Fragments arranged by size:
538 241 94
SacI G_AGCT'C
Cuts at: 0 352 374 873
Size: 352 22 499
Fragments arranged by size:
499 352 22
SfcI C'TryA_G
Cuts at: 0 63 237 331 873
Size: 63 174 94 542
Fragments arranged by size:
542 174 94 63
SmlI C'TyrA_G
Cuts at: 0 685 873
Size: 685 188
SphI G_CATG'C
Cuts at: 0 9 873
Size: 9 864
SspI AAT'ATT
Cuts at: 0 744 873
Size: 744 129
StuI AGG'CCT
Cuts at: 0 325 691 873
Size: 325 366 182
Fragments arranged by size:
366 325 182
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 419 501 873
Size: 419 82 372
Fragments arranged by size:
419 372 82
XmnI GAAnn'nnTTC
Cuts at: 0 863 873
Size: 863 10
ZraI GAC'GTC
Cuts at: 0 443 873
Size: 443 430
Enzymes that do cut:
AatII AccI AcuI AflIII AleI AlfI AlwNI ApaLI
BanII BbsI BbvCI BclI BglII Bme1580I BmgBI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BsrDI BstAPI BstYI BtsI DraIII
EaeI EarI EcoICRI Eco57MI Hin4I MfeI MmeI MscI
MslI NspI PciI PstI SacI SfcI SmlI SphI
SspI StuI XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AloI
ApaI ApoI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BcgI BciVI BfrBI BglI BlpI
BmrI BmtI BplI BsaAI BsaBI BsaXI BsiEI BsiWI
BsmI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DrdI EagI EciI EcoNI EcoO109I
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII
SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI StyI SwaI
TaqII TatI TstI Tth111I XbaI XhoI XmaI