Restriction map for
pF1KB4809
(Linear) (Six-base) MAPSORT of: KIBB4809.seq Check: 416 from: 1 to: 2202
KIBB4809.orf
With 182 enzymes: SgfI *
March 3, 2008 10:57 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 293 2202
Size: 293 1909
AccI GT'mk_AC
Cuts at: 0 1205 2202
Size: 1205 997
Acc65I G'GTAC_C
Cuts at: 0 752 1963 2202
Size: 752 1211 239
Fragments arranged by size:
1211 752 239
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 700 1192 1696 1765 2202
Size: 439 261 492 504 69 437
Fragments arranged by size:
504 492 439 437 261 69
AflIII A'CryG_T
Cuts at: 0 1208 2202
Size: 1208 994
AhdI GACnn_n'nnGTC
Cuts at: 0 432 1266 2202
Size: 432 834 936
Fragments arranged by size:
936 834 432
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 946 978 2202
Size: 946 32 1224
Fragments arranged by size:
1224 946 32
AlwNI CAG_nnn'CTG
Cuts at: 0 294 678 693 1170 1732 2202
Size: 294 384 15 477 562 470
Fragments arranged by size:
562 477 470 384 294 15
ApaI G_GGCC'C
Cuts at: 0 1779 2202
Size: 1779 423
ApoI r'AATT_y
Cuts at: 0 1275 2202
Size: 1275 927
AvaI C'yCGr_G
Cuts at: 0 562 2202
Size: 562 1640
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 744 777 2202
Size: 744 33 1425
Fragments arranged by size:
1425 744 33
BanI G'GyrC_C
Cuts at: 0 133 608 752 1963 2202
Size: 133 475 144 1211 239
Fragments arranged by size:
1211 475 239 144 133
BanII G_rGCy'C
Cuts at: 0 946 1081 1779 2202
Size: 946 135 698 423
Fragments arranged by size:
946 698 423 135
BbeI G_GCGC'C
Cuts at: 0 612 2202
Size: 612 1590
BbvCI CC'TCA_GC
Cuts at: 0 1867 2202
Size: 1867 335
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 447 481 553 587 2202
Size: 447 34 72 34 1615
Fragments arranged by size:
1615 447 72 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 566 1900 2202
Size: 566 1334 302
Fragments arranged by size:
1334 566 302
BlpI GC'TnA_GC
Cuts at: 0 2014 2202
Size: 2014 188
Bme1580I G_kGCm'C
Cuts at: 0 136 1039 1779 2202
Size: 136 903 740 423
Fragments arranged by size:
903 740 423 136
BmgBI CAC'GTC
Cuts at: 0 1211 2202
Size: 1211 991
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1020 2202
Size: 1020 1182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 346 1794 1858 2202
Size: 316 30 1448 64 344
Fragments arranged by size:
1448 344 316 64 30
Bpu10I CC'TnA_GC
Cuts at: 0 1867 2202
Size: 1867 335
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 367 2020 2182 2202
Size: 160 207 1653 162 20
Fragments arranged by size:
1653 207 162 160 20
BsaAI yAC'GTr
Cuts at: 0 1620 2202
Size: 1620 582
BsaBI GATnn'nnATC
Cuts at: 0 1552 2202
Size: 1552 650
BsaHI Gr'CG_yC
Cuts at: 0 53 392 609 2202
Size: 53 339 217 1593
Fragments arranged by size:
1593 339 217 53
BsaWI w'CCGG_w
Cuts at: 0 1960 2202
Size: 1960 242
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 946 976 1286 1316 1792 1822 2202
Size: 946 30 310 30 476 30 380
Fragments arranged by size:
946 476 380 310 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 367 967 970 973 1130 1591 1927 2202
Size: 367 600 3 3 157 461 336 275
Fragments arranged by size:
600 461 367 336 275 157 3 3
BseYI C'CCAG_C
Cuts at: 0 604 931 1163 1414 1481 2202
Size: 604 327 232 251 67 721
Fragments arranged by size:
721 604 327 251 232 67
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1600 2202
Size: 1600 602
BsiEI CG_ry'CG
Cuts at: 0 141 1479 2202
Size: 141 1338 723
Fragments arranged by size:
1338 723 141
BsiHKAI G_wGCw'C
Cuts at: 0 284 362 1571 2075 2117 2202
Size: 284 78 1209 504 42 85
Fragments arranged by size:
1209 504 284 85 78 42
BsmI GAATG_Cn'
Cuts at: 0 407 2202
Size: 407 1795
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1483 2202
Size: 1483 719
Bsp1286I G_dGCh'C
Cuts at: 0 136 284 362 946 1039 1081 1571 1779
Size: 136 148 78 584 93 42 490 208
Cuts at: 1779 2075 2117 2202
Size: 296 42 85
Fragments arranged by size:
584 490 296 208 148 136 93 85
78 42 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 293 1136 2202
Size: 293 843 1066
Fragments arranged by size:
1066 843 293
BsrBI CCG'CTC
Cuts at: 0 1772 1808 2202
Size: 1772 36 394
Fragments arranged by size:
1772 394 36
BsrDI GCAATG_nn'
Cuts at: 0 542 1055 2202
Size: 542 513 1147
Fragments arranged by size:
1147 542 513
BsrGI T'GTAC_A
Cuts at: 0 263 2202
Size: 263 1939
BssSI C'ACGA_G
Cuts at: 0 1786 2202
Size: 1786 416
BstAPI GCAn_nnn'nTGC
Cuts at: 0 693 1732 2142 2172 2202
Size: 693 1039 410 30 30
Fragments arranged by size:
1039 693 410 30 30
BstEII G'GTnAC_C
Cuts at: 0 327 786 2202
Size: 327 459 1416
Fragments arranged by size:
1416 459 327
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1305 2202
Size: 1305 897
BstYI r'GATC_y
Cuts at: 0 225 449 2159 2202
Size: 225 224 1710 43
Fragments arranged by size:
1710 225 224 43
BstZ17I GTA'TAC
Cuts at: 0 1206 2202
Size: 1206 996
BtgI C'CryG_G
Cuts at: 0 711 1298 1382 2202
Size: 711 587 84 820
Fragments arranged by size:
820 711 587 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 450 999 1839 2152 2202
Size: 450 549 840 313 50
Fragments arranged by size:
840 549 450 313 50
DraIII CAC_nnn'GTG
Cuts at: 0 444 2130 2202
Size: 444 1686 72
Fragments arranged by size:
1686 444 72
DrdI GACnn_nn'nnGTC
Cuts at: 0 373 769 1158 2202
Size: 373 396 389 1044
Fragments arranged by size:
1044 396 389 373
EaeI y'GGCC_r
Cuts at: 0 230 251 918 1307 1476 1595 1667 2202
Size: 230 21 667 389 169 119 72 535
Fragments arranged by size:
667 535 389 230 169 119 72 21
EagI C'GGCC_G
Cuts at: 0 1476 2202
Size: 1476 726
EarI CTCTTCn'nnn_
Cuts at: 0 1933 2202
Size: 1933 269
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1307 2202
Size: 1307 895
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 316 346 439 700 1192 1696 1765 1794
Size: 316 30 93 261 492 504 69 29
Cuts at: 1794 1858 2202
Size: 64 344
Fragments arranged by size:
504 492 344 316 261 93 69 64
30 29
EcoO109I rG'GnC_Cy
Cuts at: 0 246 1143 1260 1775 1917 2202
Size: 246 897 117 515 142 285
Fragments arranged by size:
897 515 285 246 142 117
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2061 2202
Size: 2061 141
FspI TGC'GCA
Cuts at: 0 1852 2202
Size: 1852 350
FspAI rTGC'GCAy
Cuts at: 0 1852 2202
Size: 1852 350
HaeII r_GCGC'y
Cuts at: 0 102 612 2043 2202
Size: 102 510 1431 159
Fragments arranged by size:
1431 510 159 102
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 210 242 418 450 1106 1138 1252 1284
Size: 210 32 176 32 656 32 114 32
Cuts at: 1284 1286 1318 1792 1824 2151 2183 2202
Size: 2 32 474 32 327 32 19
Fragments arranged by size:
656 474 327 210 176 114 32 32
32 32 32 32 32 19 2
KasI G'GCGC_C
Cuts at: 0 608 2202
Size: 608 1594
KpnI G_GTAC'C
Cuts at: 0 756 1967 2202
Size: 756 1211 235
Fragments arranged by size:
1211 756 235
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 404 1925 2202
Size: 368 36 1521 277
Fragments arranged by size:
1521 368 277 36
MscI TGG'CCA
Cuts at: 0 232 1597 1669 2202
Size: 232 1365 72 533
Fragments arranged by size:
1365 533 232 72
MslI CAynn'nnrTG
Cuts at: 0 547 898 1042 1762 1825 2202
Size: 547 351 144 720 63 377
Fragments arranged by size:
720 547 377 351 144 63
MspA1I CmG'CkG
Cuts at: 0 90 291 521 1234 1442 1481 1836 2202
Size: 90 201 230 713 208 39 355 366
Fragments arranged by size:
713 366 355 230 208 201 90 39
NarI GG'CG_CC
Cuts at: 0 609 2202
Size: 609 1593
NcoI C'CATG_G
Cuts at: 0 1298 1382 2202
Size: 1298 84 820
Fragments arranged by size:
1298 820 84
NspI r_CATG'y
Cuts at: 0 735 1606 2175 2202
Size: 735 871 569 27
Fragments arranged by size:
871 735 569 27
PasI CC'CwG_GG
Cuts at: 0 775 1147 2202
Size: 775 372 1055
Fragments arranged by size:
1055 775 372
PfoI T'CCnGG_A
Cuts at: 0 362 1270 2202
Size: 362 908 932
Fragments arranged by size:
932 908 362
PmlI CAC'GTG
Cuts at: 0 1620 2202
Size: 1620 582
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 946 978 2202
Size: 946 32 1224
Fragments arranged by size:
1224 946 32
PpuMI rG'GwC_Cy
Cuts at: 0 246 1143 1260 2202
Size: 246 897 117 942
Fragments arranged by size:
942 897 246 117
PspOMI G'GGCC_C
Cuts at: 0 1775 2202
Size: 1775 427
PspXI vC'TCGA_Gb
Cuts at: 0 562 2202
Size: 562 1640
PstI C_TGCA'G
Cuts at: 0 95 290 689 1737 1886 2138 2202
Size: 95 195 399 1048 149 252 64
Fragments arranged by size:
1048 399 252 195 149 95 64
PvuII CAG'CTG
Cuts at: 0 90 291 521 1234 1836 2202
Size: 90 201 230 713 602 366
Fragments arranged by size:
713 602 366 230 201 90
RsrII CG'GwC_CG
Cuts at: 0 480 2202
Size: 480 1722
SanDI GG'GwC_CC
Cuts at: 0 1143 1260 2202
Size: 1143 117 942
Fragments arranged by size:
1143 942 117
SapI GCTCTTCn'nnn_
Cuts at: 0 1933 2202
Size: 1933 269
ScaI AGT'ACT
Cuts at: 0 202 2202
Size: 202 2000
SexAI A'CCwGG_T
Cuts at: 0 440 2202
Size: 440 1762
SfcI C'TryA_G
Cuts at: 0 30 91 286 685 1733 1882 2134 2202
Size: 30 61 195 399 1048 149 252 68
Fragments arranged by size:
1048 399 252 195 149 68 61 30
SfoI GGC'GCC
Cuts at: 0 610 2202
Size: 610 1592
SmlI C'TyrA_G
Cuts at: 0 175 382 562 2035 2197 2202
Size: 175 207 180 1473 162 5
Fragments arranged by size:
1473 207 180 175 162 5
SphI G_CATG'C
Cuts at: 0 2175 2202
Size: 2175 27
StyI C'CwwG_G
Cuts at: 0 308 488 1298 1382 1409 2202
Size: 308 180 810 84 27 793
Fragments arranged by size:
810 793 308 180 84 27
TatI w'GTAC_w
Cuts at: 0 200 263 2202
Size: 200 63 1939
Fragments arranged by size:
1939 200 63
Tth111I GACn'n_nGTC
Cuts at: 0 766 2202
Size: 766 1436
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 621 2202
Size: 621 1581
XhoI C'TCGA_G
Cuts at: 0 562 2202
Size: 562 1640
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AloI AlwNI
ApaI ApoI AvaI BaeI BanI BanII BbeI BbvCI
BcgI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrGI BssSI BstAPI BstEII BstXI BstYI BstZ17I
BtgI BtgZI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoO109I FalI FspI FspAI HaeII Hin4I KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PfoI PmlI PpiI PpuMI PspOMI PspXI PstI
PvuII RsrII SanDI SapI ScaI SexAI SfcI SfoI
SmlI SphI StyI TatI Tth111I XcmI XhoI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI AlfI ApaLI
AscI AseI AsiSI AvrII BamHI BbsI BciVI BclI
BfrBI BglII BmrI BmtI BsaI BsiWI BspEI BspHI
BsrFI BssHII BstBI Bsu36I BtsI ClaI CspCI DraI
EcoICRI EcoNI EcoRI EcoRV FseI HincII HindIII HpaI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PciI PflMI PmeI PshAI PsiI PsrI
PvuI SacI SacII SalI SbfI SfiI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TstI XbaI XmaI XmnI ZraI