(Linear) (Six-base) MAPSORT of: KIBB4671.seq  Check: 9267  from: 1  to: 936



KIBB4671.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AflIII A'CryG_T

Cuts at:      0     430     936 
   Size:        430     506
 
ApoI r'AATT_y

Cuts at:      0     700     936 
   Size:        700     236
 
BbvCI CC'TCA_GC

Cuts at:      0     488     936 
   Size:        488     448
 
BclI T'GATC_A

Cuts at:      0     144     258     936 
   Size:        144     114     678

  Fragments arranged by size: 

                 678     144     114
 
BglII A'GATC_T

Cuts at:      0     576     936 
   Size:        576     360
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     936 
   Size:        327     609
 
Bpu10I CC'TnA_GC

Cuts at:      0     197     488     936 
   Size:        197     291     448

  Fragments arranged by size: 

                 448     291     197
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     751     936 
   Size:        751     185
 
BsaAI yAC'GTr

Cuts at:      0     431     936 
   Size:        431     505
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     172     202     302     332     859     889     936 
   Size:        172      30     100      30     527      30      47

  Fragments arranged by size: 

                 527     172     100      47      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     545     571     936 
   Size:        545      26     365

  Fragments arranged by size: 

                 545     365      26
 
BsiHKAI G_wGCw'C

Cuts at:      0      43     936 
   Size:         43     893
 
Bsp1286I G_dGCh'C

Cuts at:      0      43     936 
   Size:         43     893
 
BsrGI T'GTAC_A

Cuts at:      0     433     936 
   Size:        433     503
 
BssSI C'ACGA_G

Cuts at:      0     514     936 
   Size:        514     422
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     885     936 
   Size:        885      51
 
BstYI r'GATC_y

Cuts at:      0     576     936 
   Size:        576     360
 
BtsI GCAGTG_nn'

Cuts at:      0     392     936 
   Size:        392     544
 
DraI TTT'AAA

Cuts at:      0     276     936 
   Size:        276     660
 
EaeI y'GGCC_r

Cuts at:      0     282     936 
   Size:        282     654
 
EarI CTCTTCn'nnn_

Cuts at:      0     229     936 
   Size:        229     707
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     936 
   Size:        327     609
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      55      71      87     103     172     204     211     243 
   Size:         55      16      16      16      69      32       7      32

Cuts at:    243     798     830     859     891     936 
   Size:        555      32      29      32      45

  Fragments arranged by size: 

                 555      69      55      45      32      32      32      32
                  29      16      16      16       7
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      27     516     936 
   Size:         27     489     420

  Fragments arranged by size: 

                 489     420      27
 
MscI TGG'CCA

Cuts at:      0     284     936 
   Size:        284     652
 
NspI r_CATG'y

Cuts at:      0     722     733     936 
   Size:        722      11     203

  Fragments arranged by size: 

                 722     203      11
 
PfoI T'CCnGG_A

Cuts at:      0     306     936 
   Size:        306     630
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     820     852     936 
   Size:        820      32      84

  Fragments arranged by size: 

                 820      84      32
 
SapI GCTCTTCn'nnn_

Cuts at:      0     229     936 
   Size:        229     707
 
SfcI C'TryA_G

Cuts at:      0     639     936 
   Size:        639     297
 
SmlI C'TyrA_G

Cuts at:      0     766     936 
   Size:        766     170
 
SphI G_CATG'C

Cuts at:      0     722     936 
   Size:        722     214
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     454     936 
   Size:        454     482
 
TatI w'GTAC_w

Cuts at:      0     433     936 
   Size:        433     503
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     302     334     936 
   Size:        302      32     602

  Fragments arranged by size: 

                 602     302      32
 
XbaI T'CTAG_A

Cuts at:      0     505     936 
   Size:        505     431
 

 Enzymes that do cut:

   AflIII     ApoI    BbvCI     BclI    BglII     BpmI   Bpu10I    BpuEI
    BsaAI    BsaXI    BseRI  BsiHKAI Bsp1286I    BsrGI    BssSI    BstXI
    BstYI     BtsI     DraI     EaeI     EarI  Eco57MI    Hin4I     MmeI
     MscI     NspI     PfoI     PpiI     SapI     SfcI     SmlI     SphI
    TaqII     TatI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaHI
    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI   BssHII   BstAPI    BstBI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII
     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI    PflMI     PmeI     PmlI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SrfI     SspI     StuI
     StyI     SwaI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI