(Linear) (Six-base) MAPSORT of: KIBB4651.seq  Check: 8964  from: 1  to: 834



KIBB4651.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      89     834 
   Size:         89     745
 
AccI GT'mk_AC

Cuts at:      0     138     834 
   Size:        138     696
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27     834 
   Size:         27     807
 
ApoI r'AATT_y

Cuts at:      0     530     575     803     834 
   Size:        530      45     228      31

  Fragments arranged by size: 

                 530     228      45      31
 
BanII G_rGCy'C

Cuts at:      0     639     834 
   Size:        639     195
 
BbvCI CC'TCA_GC

Cuts at:      0     584     834 
   Size:        584     250
 
BglI GCCn_nnn'nGGC

Cuts at:      0     187     834 
   Size:        187     647
 
Bpu10I CC'TnA_GC

Cuts at:      0     584     834 
   Size:        584     250
 
BsaI GGTCTCn'nnnn_

Cuts at:      0      84     834 
   Size:         84     750
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     626     656     834 
   Size:        626      30     178

  Fragments arranged by size: 

                 626     178      30
 
BseYI C'CCAG_C

Cuts at:      0      41     146     479     834 
   Size:         41     105     333     355

  Fragments arranged by size: 

                 355     333     105      41
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     123     834 
   Size:        123     711
 
Bsp1286I G_dGCh'C

Cuts at:      0     639     834 
   Size:        639     195
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      89     357     834 
   Size:         89     268     477

  Fragments arranged by size: 

                 477     268      89
 
BsrDI GCAATG_nn'

Cuts at:      0     210     834 
   Size:        210     624
 
BstYI r'GATC_y

Cuts at:      0     109     258     834 
   Size:        109     149     576

  Fragments arranged by size: 

                 576     149     109
 
BtsI GCAGTG_nn'

Cuts at:      0     359     834 
   Size:        359     475
 
DraI TTT'AAA

Cuts at:      0     536     756     834 
   Size:        536     220      78

  Fragments arranged by size: 

                 536     220      78
 
EcoRI G'AATT_C

Cuts at:      0     575     834 
   Size:        575     259
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     396     428     546     578     595     626     627     658 
   Size:        396      32     118      32      17      31       1      31

Cuts at:    658     834 
   Size:        176

  Fragments arranged by size: 

                 396     176     118      32      32      31      31      17
                   1
 
HincII GTy'rAC

Cuts at:      0     693     834 
   Size:        693     141
 
HpaI GTT'AAC

Cuts at:      0     693     834 
   Size:        693     141
 
MslI CAynn'nnrTG

Cuts at:      0      99     834 
   Size:         99     735
 
MspA1I CmG'CkG

Cuts at:      0      79     337     583     834 
   Size:         79     258     246     251

  Fragments arranged by size: 

                 258     251     246      79
 
NspI r_CATG'y

Cuts at:      0     194     834 
   Size:        194     640
 
PstI C_TGCA'G

Cuts at:      0     303     834 
   Size:        303     531
 
PvuII CAG'CTG

Cuts at:      0      79     337     583     834 
   Size:         79     258     246     251

  Fragments arranged by size: 

                 258     251     246      79
 
SfcI C'TryA_G

Cuts at:      0      16     299     834 
   Size:         16     283     535

  Fragments arranged by size: 

                 535     283      16
 
SspI AAT'ATT

Cuts at:      0     781     834 
   Size:        781      53
 
StuI AGG'CCT

Cuts at:      0     133     834 
   Size:        133     701
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      75     138     709     834 
   Size:         75      63     571     125

  Fragments arranged by size: 

                 571     125      75      63
 
TatI w'GTAC_w

Cuts at:      0     175     834 
   Size:        175     659
 

 Enzymes that do cut:

     AarI     AccI    AlwNI     ApoI    BanII    BbvCI     BglI   Bpu10I
     BsaI    BsaXI    BseYI     BsgI Bsp1286I    BspMI    BsrDI    BstYI
     BtsI     DraI    EcoRI    Hin4I   HincII     HpaI     MslI   MspA1I
     NspI     PstI    PvuII     SfcI     SspI     StuI    TaqII     TatI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRV     FalI     FseI     FspI    FspAI    HaeII
  HindIII     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     StyI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI