(Linear) (Six-base) MAPSORT of: KIBB4616.seq  Check: 4391  from: 1  to: 1032



KIBB4616.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     709    1032 
   Size:        709     323
 
AflIII A'CryG_T

Cuts at:      0      49    1032 
   Size:         49     983
 
ApoI r'AATT_y

Cuts at:      0     484     784    1032 
   Size:        484     300     248

  Fragments arranged by size: 

                 484     300     248
 
AvaI C'yCGr_G

Cuts at:      0     752    1032 
   Size:        752     280
 
BanI G'GyrC_C

Cuts at:      0    1009    1032 
   Size:       1009      23
 
BanII G_rGCy'C

Cuts at:      0      68     618     736    1032 
   Size:         68     550     118     296

  Fragments arranged by size: 

                 550     296     118      68
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     386     434    1032 
   Size:        386      48     598

  Fragments arranged by size: 

                 598     386      48
 
BlpI GC'TnA_GC

Cuts at:      0     971    1032 
   Size:        971      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     998    1032 
   Size:        998      34
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29    1032 
   Size:         29    1003
 
BsaAI yAC'GTr

Cuts at:      0      50    1032 
   Size:         50     982
 
BsaWI w'CCGG_w

Cuts at:      0     537    1032 
   Size:        537     495
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     619     649    1032 
   Size:        619      30     383

  Fragments arranged by size: 

                 619     383      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     382     832    1032 
   Size:        382     450     200

  Fragments arranged by size: 

                 450     382     200
 
BsiHKAI G_wGCw'C

Cuts at:      0     736    1032 
   Size:        736     296
 
BsmI GAATG_Cn'

Cuts at:      0     109    1032 
   Size:        109     923
 
Bsp1286I G_dGCh'C

Cuts at:      0      68     618     736    1032 
   Size:         68     550     118     296

  Fragments arranged by size: 

                 550     296     118      68
 
BspEI T'CCGG_A

Cuts at:      0     537    1032 
   Size:        537     495
 
BsrDI GCAATG_nn'

Cuts at:      0     863    1032 
   Size:        863     169
 
BtgI C'CryG_G

Cuts at:      0     183     543    1032 
   Size:        183     360     489

  Fragments arranged by size: 

                 489     360     183
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     377    1004    1032 
   Size:        377     627      28

  Fragments arranged by size: 

                 627     377      28
 
EaeI y'GGCC_r

Cuts at:      0     170     521     860    1032 
   Size:        170     351     339     172

  Fragments arranged by size: 

                 351     339     172     170
 
EarI CTCTTCn'nnn_

Cuts at:      0     284     302     320     338    1016    1032 
   Size:        284      18      18      18     678      16

  Fragments arranged by size: 

                 678     284      18      18      18      16
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     472    1032 
   Size:        472     560
 
EcoICRI GAG'CTC

Cuts at:      0     734    1032 
   Size:        734     298
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      29     709    1032 
   Size:         29     680     323

  Fragments arranged by size: 

                 680     323      29
 
EcoO109I rG'GnC_Cy

Cuts at:      0     828    1000    1032 
   Size:        828     172      32

  Fragments arranged by size: 

                 828     172      32
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     283     301     315     333     519     551     883     915 
   Size:        283      18      14      18     186      32     332      32

Cuts at:    915    1032 
   Size:        117

  Fragments arranged by size: 

                 332     283     186     117      32      32      18      18
                  14
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     281    1032 
   Size:        281     751
 
MscI TGG'CCA

Cuts at:      0     523     862    1032 
   Size:        523     339     170

  Fragments arranged by size: 

                 523     339     170
 
MspA1I CmG'CkG

Cuts at:      0     211     970    1032 
   Size:        211     759      62

  Fragments arranged by size: 

                 759     211      62
 
NcoI C'CATG_G

Cuts at:      0     543    1032 
   Size:        543     489
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     610    1032 
   Size:        610     422
 
PmlI CAC'GTG

Cuts at:      0      50    1032 
   Size:         50     982
 
PpuMI rG'GwC_Cy

Cuts at:      0    1000    1032 
   Size:       1000      32
 
PvuII CAG'CTG

Cuts at:      0     211     970    1032 
   Size:        211     759      62

  Fragments arranged by size: 

                 759     211      62
 
SacI G_AGCT'C

Cuts at:      0     736    1032 
   Size:        736     296
 
StuI AGG'CCT

Cuts at:      0     196    1032 
   Size:        196     836
 
StyI C'CwwG_G

Cuts at:      0     543     938    1032 
   Size:        543     395      94

  Fragments arranged by size: 

                 543     395      94
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     619     651    1032 
   Size:        619      32     381

  Fragments arranged by size: 

                 619     381      32
 

 Enzymes that do cut:

     AcuI   AflIII     ApoI     AvaI     BanI    BanII     BbsI     BlpI
     BmrI     BpmI    BsaAI    BsaWI    BsaXI    BseRI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BsrDI     BtgI    BtgZI     EaeI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    Hin4I     MmeI     MscI   MspA1I     NcoI
    PflMI     PmlI    PpuMI    PvuII     SacI     StuI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaBI    BsaHI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspHI    BspMI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NaeI     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI