(Linear) (Six-base) MAPSORT of: KIBB4606.seq  Check: 2574  from: 1  to: 369



KIBB4606.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AatII G_ACGT'C

Cuts at:      0      41     369 
   Size:         41     328
 
AccI GT'mk_AC

Cuts at:      0       4     369 
   Size:          4     365
 
AlwNI CAG_nnn'CTG

Cuts at:      0      30     369 
   Size:         30     339
 
BanI G'GyrC_C

Cuts at:      0      77     369 
   Size:         77     292
 
BbeI G_GCGC'C

Cuts at:      0      81     369 
   Size:         81     288
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      33      41     369 
   Size:         33       8     328

  Fragments arranged by size: 

                 328      33       8
 
BlpI GC'TnA_GC

Cuts at:      0      95     369 
   Size:         95     274
 
BsaHI Gr'CG_yC

Cuts at:      0      38      78     369 
   Size:         38      40     291

  Fragments arranged by size: 

                 291      40      38
 
BseYI C'CCAG_C

Cuts at:      0     349     369 
   Size:        349      20
 
BsiHKAI G_wGCw'C

Cuts at:      0     211     369 
   Size:        211     158
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     219     369 
   Size:        219     150
 
Bsp1286I G_dGCh'C

Cuts at:      0     211     369 
   Size:        211     158
 
EaeI y'GGCC_r

Cuts at:      0     194     369 
   Size:        194     175
 
EcoO109I rG'GnC_Cy

Cuts at:      0     105     369 
   Size:        105     264
 
HaeII r_GCGC'y

Cuts at:      0      81     369 
   Size:         81     288
 
KasI G'GCGC_C

Cuts at:      0      77     369 
   Size:         77     292
 
MfeI C'AATT_G

Cuts at:      0      72     369 
   Size:         72     297
 
MscI TGG'CCA

Cuts at:      0     196     369 
   Size:        196     173
 
MslI CAynn'nnrTG

Cuts at:      0     163     190     202     239     369 
   Size:        163      27      12      37     130

  Fragments arranged by size: 

                 163     130      37      27      12
 
MspA1I CmG'CkG

Cuts at:      0      94     369 
   Size:         94     275
 
NarI GG'CG_CC

Cuts at:      0      78     369 
   Size:         78     291
 
PvuII CAG'CTG

Cuts at:      0      94     369 
   Size:         94     275
 
SfoI GGC'GCC

Cuts at:      0      79     369 
   Size:         79     290
 
TatI w'GTAC_w

Cuts at:      0     257     284     369 
   Size:        257      27      85

  Fragments arranged by size: 

                 257      85      27
 
XbaI T'CTAG_A

Cuts at:      0     306     369 
   Size:        306      63
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     337     369 
   Size:        337      32
 
ZraI GAC'GTC

Cuts at:      0      39     369 
   Size:         39     330
 

 Enzymes that do cut:

    AatII     AccI    AlwNI     BanI     BbeI     BbsI     BlpI    BsaHI
    BseYI  BsiHKAI    BsmBI Bsp1286I     EaeI EcoO109I    HaeII     KasI
     MfeI     MscI     MslI   MspA1I     NarI    PvuII     SfoI     TatI
     XbaI     XcmI     ZraI

 Enzymes that do not cut: 

     AarI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaWI    BsaXI    BseRI     BsgI    BsiEI    BsiWI     BsmI
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII     HpaI
     KpnI     MluI     MmeI     NaeI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TstI  Tth111I     XhoI     XmaI
     XmnI