Restriction map for
pF1KB4593
(Linear) (Six-base) MAPSORT of: KIBB4593.seq Check: 6022 from: 1 to: 2340
KIBB4593.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
AccI GT'mk_AC
Cuts at: 0 115 2340
Size: 115 2225
AclI AA'CG_TT
Cuts at: 0 466 2340
Size: 466 1874
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1673 1755 2159 2312 2340
Size: 1097 576 82 404 153 28
Fragments arranged by size:
1097 576 404 153 82 28
AfeI AGC'GCT
Cuts at: 0 433 2340
Size: 433 1907
AflIII A'CryG_T
Cuts at: 0 1596 2340
Size: 1596 744
AleI CACnn'nnGTG
Cuts at: 0 1135 2340
Size: 1135 1205
AlwNI CAG_nnn'CTG
Cuts at: 0 154 1894 1987 2340
Size: 154 1740 93 353
Fragments arranged by size:
1740 353 154 93
ApoI r'AATT_y
Cuts at: 0 25 273 403 482 541 744 775 905
Size: 25 248 130 79 59 203 31 130
Cuts at: 905 1296 1476 1545 2340
Size: 391 180 69 795
Fragments arranged by size:
795 391 248 203 180 130 130 79
69 59 31 25
AvaI C'yCGr_G
Cuts at: 0 86 2340
Size: 86 2254
BanI G'GyrC_C
Cuts at: 0 715 2340
Size: 715 1625
BanII G_rGCy'C
Cuts at: 0 918 2340
Size: 918 1422
BbsI GAAGACnn'nnnn_
Cuts at: 0 461 2340
Size: 461 1879
BclI T'GATC_A
Cuts at: 0 210 380 2172 2340
Size: 210 170 1792 168
Fragments arranged by size:
1792 210 170 168
BglII A'GATC_T
Cuts at: 0 1449 2340
Size: 1449 891
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 2340
Size: 176 2164
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 302 2340
Size: 302 2038
BsaAI yAC'GTr
Cuts at: 0 740 2340
Size: 740 1600
BsaHI Gr'CG_yC
Cuts at: 0 287 2340
Size: 287 2053
BsaWI w'CCGG_w
Cuts at: 0 1068 2340
Size: 1068 1272
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1089 1119 2340
Size: 1089 30 1221
Fragments arranged by size:
1221 1089 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 986 989 2012 2340
Size: 986 3 1023 328
Fragments arranged by size:
1023 986 328 3
BseYI C'CCAG_C
Cuts at: 0 757 2340
Size: 757 1583
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 716 2340
Size: 716 1624
BsiHKAI G_wGCw'C
Cuts at: 0 918 2340
Size: 918 1422
BsmI GAATG_Cn'
Cuts at: 0 819 2340
Size: 819 1521
Bsp1286I G_dGCh'C
Cuts at: 0 918 2340
Size: 918 1422
BspHI T'CATG_A
Cuts at: 0 1749 2340
Size: 1749 591
BsrDI GCAATG_nn'
Cuts at: 0 687 1946 2340
Size: 687 1259 394
Fragments arranged by size:
1259 687 394
BstYI r'GATC_y
Cuts at: 0 14 1449 2319 2340
Size: 14 1435 870 21
Fragments arranged by size:
1435 870 21 14
Bsu36I CC'TnA_GG
Cuts at: 0 19 2340
Size: 19 2321
BtsI GCAGTG_nn'
Cuts at: 0 699 1500 2340
Size: 699 801 840
Fragments arranged by size:
840 801 699
ClaI AT'CG_AT
Cuts at: 0 2292 2340
Size: 2292 48
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1076 1111 2259 2294 2340
Size: 1076 35 1148 35 46
Fragments arranged by size:
1148 1076 46 35 35
DraI TTT'AAA
Cuts at: 0 480 2340
Size: 480 1860
EaeI y'GGCC_r
Cuts at: 0 589 2340
Size: 589 1751
EarI CTCTTCn'nnn_
Cuts at: 0 46 1774 2340
Size: 46 1728 566
Fragments arranged by size:
1728 566 46
EcoICRI GAG'CTC
Cuts at: 0 916 2340
Size: 916 1424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 1097 1673 1755 2159 2312 2340
Size: 176 921 576 82 404 153 28
Fragments arranged by size:
921 576 404 176 153 82 28
EcoO109I rG'GnC_Cy
Cuts at: 0 322 1016 1320 2340
Size: 322 694 304 1020
Fragments arranged by size:
1020 694 322 304
EcoRI G'AATT_C
Cuts at: 0 1476 1545 2340
Size: 1476 69 795
Fragments arranged by size:
1476 795 69
EcoRV GAT'ATC
Cuts at: 0 1671 2340
Size: 1671 669
HaeII r_GCGC'y
Cuts at: 0 435 2340
Size: 435 1905
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 639 671 1089 1121 1408 1440 1804 1836
Size: 639 32 418 32 287 32 364 32
Cuts at: 1836 2340
Size: 504
Fragments arranged by size:
639 504 418 364 287 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 639 2340
Size: 639 1701
MfeI C'AATT_G
Cuts at: 0 1265 2340
Size: 1265 1075
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1147 2284 2340
Size: 1147 1137 56
Fragments arranged by size:
1147 1137 56
MscI TGG'CCA
Cuts at: 0 591 2340
Size: 591 1749
MslI CAynn'nnrTG
Cuts at: 0 388 668 692 1135 1808 1982 2340
Size: 388 280 24 443 673 174 358
Fragments arranged by size:
673 443 388 358 280 174 24
MspA1I CmG'CkG
Cuts at: 0 2182 2340
Size: 2182 158
NdeI CA'TA_TG
Cuts at: 0 306 1181 2340
Size: 306 875 1159
Fragments arranged by size:
1159 875 306
NspI r_CATG'y
Cuts at: 0 1381 1600 2340
Size: 1381 219 740
Fragments arranged by size:
1381 740 219
PciI A'CATG_T
Cuts at: 0 1596 2340
Size: 1596 744
PpuMI rG'GwC_Cy
Cuts at: 0 322 1016 1320 2340
Size: 322 694 304 1020
Fragments arranged by size:
1020 694 322 304
PsiI TTA'TAA
Cuts at: 0 280 2340
Size: 280 2060
PstI C_TGCA'G
Cuts at: 0 828 1064 1495 2340
Size: 828 236 431 845
Fragments arranged by size:
845 828 431 236
SacI G_AGCT'C
Cuts at: 0 918 2340
Size: 918 1422
ScaI AGT'ACT
Cuts at: 0 1908 2340
Size: 1908 432
SfcI C'TryA_G
Cuts at: 0 824 1060 1224 1491 2340
Size: 824 236 164 267 849
Fragments arranged by size:
849 824 267 236 164
SmaI CCC'GGG
Cuts at: 0 88 2340
Size: 88 2252
SmlI C'TyrA_G
Cuts at: 0 317 2340
Size: 317 2023
SpeI A'CTAG_T
Cuts at: 0 1903 2340
Size: 1903 437
SspI AAT'ATT
Cuts at: 0 1869 2340
Size: 1869 471
StyI C'CwwG_G
Cuts at: 0 1012 1561 2340
Size: 1012 549 779
Fragments arranged by size:
1012 779 549
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1104 2340
Size: 1104 1236
TatI w'GTAC_w
Cuts at: 0 828 1906 2340
Size: 828 1078 434
Fragments arranged by size:
1078 828 434
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 411 443 2340
Size: 411 32 1897
Fragments arranged by size:
1897 411 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2330 2340
Size: 2330 10
XmaI C'CCGG_G
Cuts at: 0 86 2340
Size: 86 2254
XmnI GAAnn'nnTTC
Cuts at: 0 671 854 2340
Size: 671 183 1486
Fragments arranged by size:
1486 671 183
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AleI AlwNI ApoI
AvaI BanI BanII BbsI BclI BglII BpmI BpuEI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BstYI Bsu36I BtsI ClaI
CspCI DraI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV HaeII Hin4I HindIII MfeI MmeI MscI MslI
MspA1I NdeI NspI PciI PpuMI PsiI PstI SacI
ScaI SfcI SmaI SmlI SpeI SspI StyI TaqII
TatI TstI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BfrBI BglI BlpI Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I BtgI BtgZI
DraIII DrdI EagI EciI EcoNI FalI FseI FspI
FspAI HincII HpaI KasI KpnI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SfoI SgfI SgrAI SnaBI SphI
SrfI StuI SwaI Tth111I XbaI XhoI ZraI