Restriction map for
pF1KB4575
(Linear) (Six-base) MAPSORT of: KIBB4575.seq Check: 9800 from: 1 to: 2655
KIBB4575.orf
With 182 enzymes: SgfI *
February 28, 2007 11:26 ..
Acc65I G'GTAC_C
Cuts at: 0 1308 1923 2655
Size: 1308 615 732
Fragments arranged by size:
1308 732 615
AclI AA'CG_TT
Cuts at: 0 426 1068 1225 2655
Size: 426 642 157 1430
Fragments arranged by size:
1430 642 426 157
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 458 732 1175 1342 1663 2655
Size: 139 319 274 443 167 321 992
Fragments arranged by size:
992 443 321 319 274 167 139
AfeI AGC'GCT
Cuts at: 0 2569 2655
Size: 2569 86
AflIII A'CryG_T
Cuts at: 0 540 2655
Size: 540 2115
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1409 2655
Size: 1409 1246
AlwNI CAG_nnn'CTG
Cuts at: 0 1747 2655
Size: 1747 908
ApaLI G'TGCA_C
Cuts at: 0 1894 2655
Size: 1894 761
ApoI r'AATT_y
Cuts at: 0 905 1107 1250 1458 2655
Size: 905 202 143 208 1197
Fragments arranged by size:
1197 905 208 202 143
AvaI C'yCGr_G
Cuts at: 0 171 2655
Size: 171 2484
BanI G'GyrC_C
Cuts at: 0 1308 1923 2655
Size: 1308 615 732
Fragments arranged by size:
1308 732 615
BanII G_rGCy'C
Cuts at: 0 328 1594 2655
Size: 328 1266 1061
Fragments arranged by size:
1266 1061 328
BbsI GAAGACnn'nnnn_
Cuts at: 0 2543 2655
Size: 2543 112
BlpI GC'TnA_GC
Cuts at: 0 2564 2655
Size: 2564 91
Bme1580I G_kGCm'C
Cuts at: 0 489 985 1898 2655
Size: 489 496 913 757
Fragments arranged by size:
913 757 496 489
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1589 2655
Size: 1589 1066
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1402 1874 2543 2655
Size: 917 485 472 669 112
Fragments arranged by size:
917 669 485 472 112
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 312 2592 2655
Size: 312 2280 63
Fragments arranged by size:
2280 312 63
BsaBI GATnn'nnATC
Cuts at: 0 609 2264 2655
Size: 609 1655 391
Fragments arranged by size:
1655 609 391
BsaHI Gr'CG_yC
Cuts at: 0 2166 2655
Size: 2166 489
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1786 1816 2655
Size: 1786 30 839
Fragments arranged by size:
1786 839 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 159 336 727 2478 2524 2655
Size: 159 177 391 1751 46 131
Fragments arranged by size:
1751 391 177 159 131 46
BseYI C'CCAG_C
Cuts at: 0 792 2655
Size: 792 1863
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1584 1766 2655
Size: 1584 182 889
Fragments arranged by size:
1584 889 182
BsiHKAI G_wGCw'C
Cuts at: 0 328 783 1575 1594 1898 1964 2007 2655
Size: 328 455 792 19 304 66 43 648
Fragments arranged by size:
792 648 455 328 304 66 43 19
BsmI GAATG_Cn'
Cuts at: 0 1398 2655
Size: 1398 1257
Bsp1286I G_dGCh'C
Cuts at: 0 328 489 783 985 1575 1594 1898 1964
Size: 328 161 294 202 590 19 304 66
Cuts at: 1964 2007 2655
Size: 43 648
Fragments arranged by size:
648 590 328 304 294 202 161 66
43 19
BspHI T'CATG_A
Cuts at: 0 1773 2169 2385 2655
Size: 1773 396 216 270
Fragments arranged by size:
1773 396 270 216
BsrDI GCAATG_nn'
Cuts at: 0 1368 2655
Size: 1368 1287
BstXI CCAn_nnnn'nTGG
Cuts at: 0 27 2655
Size: 27 2628
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2516 2655
Size: 2516 139
BtsI GCAGTG_nn'
Cuts at: 0 1704 2412 2655
Size: 1704 708 243
Fragments arranged by size:
1704 708 243
DraI TTT'AAA
Cuts at: 0 996 1073 2655
Size: 996 77 1582
Fragments arranged by size:
1582 996 77
DrdI GACnn_nn'nnGTC
Cuts at: 0 751 2655
Size: 751 1904
EarI CTCTTCn'nnn_
Cuts at: 0 2083 2162 2223 2496 2501 2558 2619 2655
Size: 2083 79 61 273 5 57 61 36
Fragments arranged by size:
2083 273 79 61 61 57 36 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 207 2655
Size: 207 2448
EcoICRI GAG'CTC
Cuts at: 0 326 1592 2655
Size: 326 1266 1063
Fragments arranged by size:
1266 1063 326
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 458 732 917 1175 1342 1402 1663
Size: 139 319 274 185 258 167 60 261
Cuts at: 1663 1874 2543 2655
Size: 211 669 112
Fragments arranged by size:
669 319 274 261 258 211 185 167
139 112 60
EcoNI CCTnn'n_nnAGG
Cuts at: 0 389 1565 2655
Size: 389 1176 1090
Fragments arranged by size:
1176 1090 389
EcoO109I rG'GnC_Cy
Cuts at: 0 796 1939 2655
Size: 796 1143 716
Fragments arranged by size:
1143 796 716
EcoRI G'AATT_C
Cuts at: 0 1250 2655
Size: 1250 1405
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1870 2655
Size: 1870 785
HaeII r_GCGC'y
Cuts at: 0 311 2571 2655
Size: 311 2260 84
Fragments arranged by size:
2260 311 84
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 133 165 1105 1137 2470 2473 2502 2505
Size: 133 32 940 32 1333 3 29 3
Cuts at: 2505 2655
Size: 150
Fragments arranged by size:
1333 940 150 133 32 32 29 3
3
HincII GTy'rAC
Cuts at: 0 848 1229 2655
Size: 848 381 1426
Fragments arranged by size:
1426 848 381
HindIII A'AGCT_T
Cuts at: 0 1663 2281 2655
Size: 1663 618 374
Fragments arranged by size:
1663 618 374
HpaI GTT'AAC
Cuts at: 0 1229 2655
Size: 1229 1426
KpnI G_GTAC'C
Cuts at: 0 1312 1927 2655
Size: 1312 615 728
Fragments arranged by size:
1312 728 615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1289 2565 2655
Size: 1289 1276 90
Fragments arranged by size:
1289 1276 90
MslI CAynn'nnrTG
Cuts at: 0 31 1811 1822 2390 2655
Size: 31 1780 11 568 265
Fragments arranged by size:
1780 568 265 31 11
MspA1I CmG'CkG
Cuts at: 0 1517 1734 2655
Size: 1517 217 921
Fragments arranged by size:
1517 921 217
NspI r_CATG'y
Cuts at: 0 544 723 869 1827 2655
Size: 544 179 146 958 828
Fragments arranged by size:
958 828 544 179 146
PciI A'CATG_T
Cuts at: 0 540 2655
Size: 540 2115
PflMI CCAn_nnn'nTGG
Cuts at: 0 208 2655
Size: 208 2447
PfoI T'CCnGG_A
Cuts at: 0 1905 2655
Size: 1905 750
PpuMI rG'GwC_Cy
Cuts at: 0 796 1939 2655
Size: 796 1143 716
Fragments arranged by size:
1143 796 716
PshAI GACnn'nnGTC
Cuts at: 0 2453 2655
Size: 2453 202
PsiI TTA'TAA
Cuts at: 0 811 2655
Size: 811 1844
PstI C_TGCA'G
Cuts at: 0 1739 1757 2407 2655
Size: 1739 18 650 248
Fragments arranged by size:
1739 650 248 18
PvuII CAG'CTG
Cuts at: 0 1734 2655
Size: 1734 921
SacI G_AGCT'C
Cuts at: 0 328 1594 2655
Size: 328 1266 1061
Fragments arranged by size:
1266 1061 328
SanDI GG'GwC_CC
Cuts at: 0 1939 2655
Size: 1939 716
ScaI AGT'ACT
Cuts at: 0 1427 2655
Size: 1427 1228
SfcI C'TryA_G
Cuts at: 0 1735 1753 2403 2451 2597 2655
Size: 1735 18 650 48 146 58
Fragments arranged by size:
1735 650 146 58 48 18
SmlI C'TyrA_G
Cuts at: 0 327 2571 2655
Size: 327 2244 84
Fragments arranged by size:
2244 327 84
SspI AAT'ATT
Cuts at: 0 590 2655
Size: 590 2065
StuI AGG'CCT
Cuts at: 0 384 1651 2655
Size: 384 1267 1004
Fragments arranged by size:
1267 1004 384
StyI C'CwwG_G
Cuts at: 0 221 2655
Size: 221 2434
TatI w'GTAC_w
Cuts at: 0 758 1425 2473 2655
Size: 758 667 1048 182
Fragments arranged by size:
1048 758 667 182
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 762 794 1786 1818 1841 1873 2105 2137
Size: 762 32 992 32 23 32 232 32
Cuts at: 2137 2655
Size: 518
Fragments arranged by size:
992 762 518 232 32 32 32 32
23
Tth111I GACn'n_nGTC
Cuts at: 0 188 2655
Size: 188 2467
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 27 2203 2655
Size: 27 2176 452
Fragments arranged by size:
2176 452 27
XmnI GAAnn'nnTTC
Cuts at: 0 457 529 1188 2655
Size: 457 72 659 1467
Fragments arranged by size:
1467 659 457 72
Enzymes that do cut:
Acc65I AclI AcuI AfeI AflIII AlfI AlwNI ApaLI
ApoI AvaI BanI BanII BbsI BlpI Bme1580I BplI
BpmI BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspHI BsrDI BstXI BtgZI BtsI
DraI DrdI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HincII HindIII HpaI KpnI
MmeI MslI MspA1I NspI PciI PflMI PfoI PpuMI
PshAI PsiI PstI PvuII SacI SanDI ScaI SfcI
SmlI SspI StuI StyI TatI TstI Tth111I XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI AflII AgeI AhdI AleI AloI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BmgBI
BmrI BmtI Bpu10I BsaI BsaAI BsaWI BsiEI BsiWI
BsmBI BspEI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI ClaI
CspCI DraIII EaeI EagI EcoRV FseI FspI FspAI
KasI MfeI MluI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PmlI PpiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SwaI TaqII XbaI
XhoI XmaI ZraI