Restriction map for
pF1KB4569
(Linear) (Six-base) MAPSORT of: KIBB4569.seq Check: 5605 from: 1 to: 1644
KIBB4569.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 351 1396 1600 1644
Size: 351 1045 204 44
Fragments arranged by size:
1045 351 204 44
AccI GT'mk_AC
Cuts at: 0 1352 1644
Size: 1352 292
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 478 1644
Size: 74 404 1166
Fragments arranged by size:
1166 404 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1261 1644
Size: 1261 383
AlwNI CAG_nnn'CTG
Cuts at: 0 1487 1644
Size: 1487 157
ApaI G_GGCC'C
Cuts at: 0 1374 1644
Size: 1374 270
ApoI r'AATT_y
Cuts at: 0 43 241 1644
Size: 43 198 1403
Fragments arranged by size:
1403 198 43
BamHI G'GATC_C
Cuts at: 0 524 1644
Size: 524 1120
BanI G'GyrC_C
Cuts at: 0 321 1384 1644
Size: 321 1063 260
Fragments arranged by size:
1063 321 260
BanII G_rGCy'C
Cuts at: 0 861 1374 1644
Size: 861 513 270
Fragments arranged by size:
861 513 270
BbsI GAAGACnn'nnnn_
Cuts at: 0 905 1207 1644
Size: 905 302 437
Fragments arranged by size:
905 437 302
BbvCI CC'TCA_GC
Cuts at: 0 310 1644
Size: 310 1334
BfrBI ATG'CAT
Cuts at: 0 982 1644
Size: 982 662
BglI GCCn_nnn'nGGC
Cuts at: 0 548 1644
Size: 548 1096
Bme1580I G_kGCm'C
Cuts at: 0 170 1374 1387 1644
Size: 170 1204 13 257
Fragments arranged by size:
1204 257 170 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 567 1644
Size: 567 1077
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 873 1035 1334 1644
Size: 873 162 299 310
Fragments arranged by size:
873 310 299 162
Bpu10I CC'TnA_GC
Cuts at: 0 22 310 1644
Size: 22 288 1334
Fragments arranged by size:
1334 288 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 1525 1644
Size: 1525 119
BsaAI yAC'GTr
Cuts at: 0 126 1644
Size: 126 1518
BsaBI GATnn'nnATC
Cuts at: 0 304 1644
Size: 304 1340
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 578 733 736 1123 1644
Size: 19 559 155 3 387 521
Fragments arranged by size:
559 521 387 155 19 3
BseYI C'CCAG_C
Cuts at: 0 697 870 1644
Size: 697 173 774
Fragments arranged by size:
774 697 173
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1628 1644
Size: 1628 16
BsiEI CG_ry'CG
Cuts at: 0 1593 1644
Size: 1593 51
BsiHKAI G_wGCw'C
Cuts at: 0 536 861 1277 1416 1644
Size: 536 325 416 139 228
Fragments arranged by size:
536 416 325 228 139
BsmI GAATG_Cn'
Cuts at: 0 982 1604 1644
Size: 982 622 40
Fragments arranged by size:
982 622 40
Bsp1286I G_dGCh'C
Cuts at: 0 170 536 861 1277 1374 1387 1416 1644
Size: 170 366 325 416 97 13 29 228
Fragments arranged by size:
416 366 325 228 170 97 29 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 351 1396 1600 1644
Size: 351 1045 204 44
Fragments arranged by size:
1045 351 204 44
BsrBI CCG'CTC
Cuts at: 0 741 1644
Size: 741 903
BsrDI GCAATG_nn'
Cuts at: 0 333 367 1292 1644
Size: 333 34 925 352
Fragments arranged by size:
925 352 333 34
BsrFI r'CCGG_y
Cuts at: 0 33 1644
Size: 33 1611
BstXI CCAn_nnnn'nTGG
Cuts at: 0 870 1644
Size: 870 774
BstYI r'GATC_y
Cuts at: 0 524 1644
Size: 524 1120
Bsu36I CC'TnA_GG
Cuts at: 0 1346 1644
Size: 1346 298
BtgI C'CryG_G
Cuts at: 0 293 1205 1358 1644
Size: 293 912 153 286
Fragments arranged by size:
912 293 286 153
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1416 1644
Size: 1416 228
BtsI GCAGTG_nn'
Cuts at: 0 224 1644
Size: 224 1420
EaeI y'GGCC_r
Cuts at: 0 1590 1644
Size: 1590 54
EagI C'GGCC_G
Cuts at: 0 1590 1644
Size: 1590 54
EarI CTCTTCn'nnn_
Cuts at: 0 1027 1294 1644
Size: 1027 267 350
Fragments arranged by size:
1027 350 267
EcoICRI GAG'CTC
Cuts at: 0 859 1644
Size: 859 785
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 478 873 1035 1334 1644
Size: 74 404 395 162 299 310
Fragments arranged by size:
404 395 310 299 162 74
EcoNI CCTnn'n_nnAGG
Cuts at: 0 834 1105 1644
Size: 834 271 539
Fragments arranged by size:
834 539 271
EcoO109I rG'GnC_Cy
Cuts at: 0 540 918 1371 1644
Size: 540 378 453 273
Fragments arranged by size:
540 453 378 273
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 784 816 1644
Size: 784 32 828
Fragments arranged by size:
828 784 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1036 1242 1644
Size: 1036 206 402
Fragments arranged by size:
1036 402 206
MslI CAynn'nnrTG
Cuts at: 0 298 1644
Size: 298 1346
MspA1I CmG'CkG
Cuts at: 0 30 163 330 733 870 1394 1644
Size: 30 133 167 403 137 524 250
Fragments arranged by size:
524 403 250 167 137 133 30
NcoI C'CATG_G
Cuts at: 0 293 1205 1644
Size: 293 912 439
Fragments arranged by size:
912 439 293
NdeI CA'TA_TG
Cuts at: 0 1332 1644
Size: 1332 312
NsiI A_TGCA'T
Cuts at: 0 984 1644
Size: 984 660
PflMI CCAn_nnn'nTGG
Cuts at: 0 416 1584 1644
Size: 416 1168 60
Fragments arranged by size:
1168 416 60
PmlI CAC'GTG
Cuts at: 0 126 1644
Size: 126 1518
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1618 1644
Size: 1618 26
PpuMI rG'GwC_Cy
Cuts at: 0 918 1644
Size: 918 726
PspOMI G'GGCC_C
Cuts at: 0 1370 1644
Size: 1370 274
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 580 612 1644
Size: 580 32 1032
Fragments arranged by size:
1032 580 32
PstI C_TGCA'G
Cuts at: 0 1240 1393 1553 1644
Size: 1240 153 160 91
Fragments arranged by size:
1240 160 153 91
PvuII CAG'CTG
Cuts at: 0 30 163 330 733 1644
Size: 30 133 167 403 911
Fragments arranged by size:
911 403 167 133 30
SacI G_AGCT'C
Cuts at: 0 861 1644
Size: 861 783
SexAI A'CCwGG_T
Cuts at: 0 1618 1644
Size: 1618 26
SfcI C'TryA_G
Cuts at: 0 1067 1236 1389 1549 1644
Size: 1067 169 153 160 95
Fragments arranged by size:
1067 169 160 153 95
StyI C'CwwG_G
Cuts at: 0 170 293 1205 1644
Size: 170 123 912 439
Fragments arranged by size:
912 439 170 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 917 1399 1644
Size: 917 482 245
Fragments arranged by size:
917 482 245
TatI w'GTAC_w
Cuts at: 0 482 1644
Size: 482 1162
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1114 1146 1256 1288 1644
Size: 1114 32 110 32 356
Fragments arranged by size:
1114 356 110 32 32
Enzymes that do cut:
AarI AccI AcuI AlfI AlwNI ApaI ApoI BamHI
BanI BanII BbsI BbvCI BfrBI BglI Bme1580I BmrI
BpmI Bpu10I BsaI BsaAI BsaBI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI BsrFI
BstXI BstYI Bsu36I BtgI BtgZI BtsI EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I Hin4I MmeI MslI
MspA1I NcoI NdeI NsiI PflMI PmlI PpiI PpuMI
PspOMI PsrI PstI PvuII SacI SexAI SfcI StyI
TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BbeI BcgI BciVI BclI BglII BlpI BmgBI
BmtI BplI BpuEI BsaHI BsaWI BsaXI BsiWI BsmBI
BspEI BspHI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I ClaI CspCI DraI DraIII DrdI EciI EcoRI
EcoRV FalI FseI FspI FspAI HaeII HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NgoMIV NheI NotI NruI NspI PacI PasI PciI
PfoI PmeI PshAI PsiI PspXI PvuI RsrII SacII
SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI