Restriction map for
pF1KB4568
(Linear) (Six-base) MAPSORT of: KIBB4568.seq Check: 5254 from: 1 to: 1110
KIBB4568.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AclI AA'CG_TT
Cuts at: 0 417 1110
Size: 417 693
AleI CACnn'nnGTG
Cuts at: 0 517 1110
Size: 517 593
ApoI r'AATT_y
Cuts at: 0 778 1110
Size: 778 332
AseI AT'TA_AT
Cuts at: 0 296 1110
Size: 296 814
BamHI G'GATC_C
Cuts at: 0 266 1110
Size: 266 844
BanI G'GyrC_C
Cuts at: 0 185 461 475 1110
Size: 185 276 14 635
Fragments arranged by size:
635 276 185 14
BanII G_rGCy'C
Cuts at: 0 157 343 1110
Size: 157 186 767
Fragments arranged by size:
767 186 157
BbeI G_GCGC'C
Cuts at: 0 189 465 1110
Size: 189 276 645
Fragments arranged by size:
645 276 189
BbsI GAAGACnn'nnnn_
Cuts at: 0 237 1110
Size: 237 873
BglI GCCn_nnn'nGGC
Cuts at: 0 31 206 226 1110
Size: 31 175 20 884
Fragments arranged by size:
884 175 31 20
BglII A'GATC_T
Cuts at: 0 289 1110
Size: 289 821
BlpI GC'TnA_GC
Cuts at: 0 1013 1110
Size: 1013 97
BmrI ACTGGGnnnn_n'
Cuts at: 0 651 1110
Size: 651 459
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 365 616 758 964 1110
Size: 347 18 251 142 206 146
Fragments arranged by size:
347 251 206 146 142 18
BsaHI Gr'CG_yC
Cuts at: 0 186 242 462 1110
Size: 186 56 220 648
Fragments arranged by size:
648 220 186 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 265 295 1110
Size: 265 30 815
Fragments arranged by size:
815 265 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 160 274 289 292 1110
Size: 160 114 15 3 818
Fragments arranged by size:
818 160 114 15 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 937 1110
Size: 178 759 173
Fragments arranged by size:
759 178 173
BsiEI CG_ry'CG
Cuts at: 0 138 177 201 1110
Size: 138 39 24 909
Fragments arranged by size:
909 138 39 24
BsiHKAI G_wGCw'C
Cuts at: 0 157 343 1110
Size: 157 186 767
Fragments arranged by size:
767 186 157
Bsp1286I G_dGCh'C
Cuts at: 0 157 343 1110
Size: 157 186 767
Fragments arranged by size:
767 186 157
BsrFI r'CCGG_y
Cuts at: 0 188 238 1110
Size: 188 50 872
Fragments arranged by size:
872 188 50
BssHII G'CGCG_C
Cuts at: 0 108 110 1110
Size: 108 2 1000
Fragments arranged by size:
1000 108 2
BstEII G'GTnAC_C
Cuts at: 0 645 1110
Size: 645 465
BstXI CCAn_nnnn'nTGG
Cuts at: 0 981 1110
Size: 981 129
BstYI r'GATC_y
Cuts at: 0 266 289 1110
Size: 266 23 821
Fragments arranged by size:
821 266 23
BtgI C'CryG_G
Cuts at: 0 7 1110
Size: 7 1103
EaeI y'GGCC_r
Cuts at: 0 20 68 135 174 198 207 959 1110
Size: 20 48 67 39 24 9 752 151
Fragments arranged by size:
752 151 67 48 39 24 20 9
EagI C'GGCC_G
Cuts at: 0 135 174 198 1110
Size: 135 39 24 912
Fragments arranged by size:
912 135 39 24
EcoICRI GAG'CTC
Cuts at: 0 155 341 1110
Size: 155 186 769
Fragments arranged by size:
769 186 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 347 365 616 758 964 1110
Size: 347 18 251 142 206 146
Fragments arranged by size:
347 251 206 146 142 18
EcoO109I rG'GnC_Cy
Cuts at: 0 51 1110
Size: 51 1059
EcoRI G'AATT_C
Cuts at: 0 778 1110
Size: 778 332
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 462 1110
Size: 462 648
HaeII r_GCGC'y
Cuts at: 0 64 189 465 1110
Size: 64 125 276 645
Fragments arranged by size:
645 276 125 64
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1110
Size: 991 32 87
Fragments arranged by size:
991 87 32
KasI G'GCGC_C
Cuts at: 0 185 461 1110
Size: 185 276 649
Fragments arranged by size:
649 276 185
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 892 1110
Size: 293 599 218
Fragments arranged by size:
599 293 218
MscI TGG'CCA
Cuts at: 0 70 209 961 1110
Size: 70 139 752 149
Fragments arranged by size:
752 149 139 70
MslI CAynn'nnrTG
Cuts at: 0 517 727 758 1110
Size: 517 210 31 352
Fragments arranged by size:
517 352 210 31
MspA1I CmG'CkG
Cuts at: 0 9 549 1082 1110
Size: 9 540 533 28
Fragments arranged by size:
540 533 28 9
NaeI GCC'GGC
Cuts at: 0 190 240 1110
Size: 190 50 870
Fragments arranged by size:
870 190 50
NarI GG'CG_CC
Cuts at: 0 186 462 1110
Size: 186 276 648
Fragments arranged by size:
648 276 186
NdeI CA'TA_TG
Cuts at: 0 798 1110
Size: 798 312
NgoMIV G'CCGG_C
Cuts at: 0 188 238 1110
Size: 188 50 872
Fragments arranged by size:
872 188 50
NspI r_CATG'y
Cuts at: 0 933 1110
Size: 933 177
PfoI T'CCnGG_A
Cuts at: 0 269 941 1110
Size: 269 672 169
Fragments arranged by size:
672 269 169
PvuII CAG'CTG
Cuts at: 0 549 1082 1110
Size: 549 533 28
Fragments arranged by size:
549 533 28
SacI G_AGCT'C
Cuts at: 0 157 343 1110
Size: 157 186 767
Fragments arranged by size:
767 186 157
SacII CC_GC'GG
Cuts at: 0 10 1110
Size: 10 1100
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 206 1110
Size: 206 904
SfoI GGC'GCC
Cuts at: 0 187 463 1110
Size: 187 276 647
Fragments arranged by size:
647 276 187
SphI G_CATG'C
Cuts at: 0 933 1110
Size: 933 177
StyI C'CwwG_G
Cuts at: 0 1040 1110
Size: 1040 70
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 493 1110
Size: 493 617
TatI w'GTAC_w
Cuts at: 0 668 1110
Size: 668 442
Enzymes that do cut:
AclI AleI ApoI AseI BamHI BanI BanII BbeI
BbsI BglI BglII BlpI BmrI BpmI BsaHI BsaXI
BseRI BsgI BsiEI BsiHKAI Bsp1286I BsrFI BssHII BstEII
BstXI BstYI BtgI EaeI EagI EcoICRI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I KasI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PfoI PvuII
SacI SacII SfiI SfoI SphI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI
AsiSI AvaI AvrII BaeI BbvCI BcgI BciVI BclI
BfrBI Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaWI BseYI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EarI EciI EcoNI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI NcoI NheI
NotI NruI NsiI PacI PasI PciI PflMI PmeI
PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SalI SanDI SapI SbfI ScaI
SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI