(Linear) (Six-base) MAPSORT of: KIBB4553.seq  Check: 5981  from: 1  to: 435



KIBB4553.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

ApaI G_GGCC'C

Cuts at:      0      28     435 
   Size:         28     407
 
BanII G_rGCy'C

Cuts at:      0      20      28     162     435 
   Size:         20       8     134     273

  Fragments arranged by size: 

                 273     134      20       8
 
BlpI GC'TnA_GC

Cuts at:      0      52     435 
   Size:         52     383
 
Bme1580I G_kGCm'C

Cuts at:      0      28     135     266     435 
   Size:         28     107     131     169

  Fragments arranged by size: 

                 169     131     107      28
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      23      86     435 
   Size:         23      63     349

  Fragments arranged by size: 

                 349      63      23
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      72     102     435 
   Size:         72      30     333

  Fragments arranged by size: 

                 333      72      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     152     435 
   Size:        152     283
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      28     135     162     266     435 
   Size:         20       8     107      27     104     169

  Fragments arranged by size: 

                 169     107     104      27      20       8
 
BsrDI GCAATG_nn'

Cuts at:      0     416     435 
   Size:        416      19
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     270     435 
   Size:        270     165
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     312     411     435 
   Size:        312      99      24

  Fragments arranged by size: 

                 312      99      24
 
BtsI GCAGTG_nn'

Cuts at:      0     264     435 
   Size:        264     171
 
EaeI y'GGCC_r

Cuts at:      0     224     413     435 
   Size:        224     189      22

  Fragments arranged by size: 

                 224     189      22
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     322     435 
   Size:        322     113
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24      25     435 
   Size:         24       1     410

  Fragments arranged by size: 

                 410      24       1
 
FspI TGC'GCA

Cuts at:      0     382     435 
   Size:        382      53
 
HaeII r_GCGC'y

Cuts at:      0     253     435 
   Size:        253     182
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     329     435 
   Size:        329     106
 
MscI TGG'CCA

Cuts at:      0     226     415     435 
   Size:        226     189      20

  Fragments arranged by size: 

                 226     189      20
 
NspI r_CATG'y

Cuts at:      0     273     435 
   Size:        273     162
 
PasI CC'CwG_GG

Cuts at:      0     135     435 
   Size:        135     300
 
PspOMI G'GGCC_C

Cuts at:      0      24     435 
   Size:         24     411
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     133     165     435 
   Size:        133      32     270

  Fragments arranged by size: 

                 270     133      32
 
SphI G_CATG'C

Cuts at:      0     273     435 
   Size:        273     162
 
StyI C'CwwG_G

Cuts at:      0     404     435 
   Size:        404      31
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     378     435 
   Size:        378      57
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      72     104     435 
   Size:         72      32     331

  Fragments arranged by size: 

                 331      72      32
 

 Enzymes that do cut:

     ApaI    BanII     BlpI Bme1580I     BmrI    BsaXI    BseRI Bsp1286I
    BsrDI   BstAPI    BstXI     BtsI     EaeI    EcoNI EcoO109I     FspI
    HaeII     MmeI     MscI     NspI     PasI   PspOMI     PsrI     SphI
     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoRI    EcoRV     FalI
     FseI    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI    PspXI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI     StuI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI