Restriction map for
pF1KB4518
(Linear) (Six-base) MAPSORT of: KIBB4518.seq Check: 738 from: 1 to: 1014
KIBB4518.orf
With 182 enzymes: SgfI *
April 24, 2009 13:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 328 1014
Size: 328 686
AhdI GACnn_n'nnGTC
Cuts at: 0 222 1014
Size: 222 792
ApoI r'AATT_y
Cuts at: 0 736 1014
Size: 736 278
AvaI C'yCGr_G
Cuts at: 0 170 356 1014
Size: 170 186 658
Fragments arranged by size:
658 186 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 18 1014
Size: 18 996
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 586 620 1014
Size: 586 34 394
Fragments arranged by size:
586 394 34
BclI T'GATC_A
Cuts at: 0 242 1014
Size: 242 772
BglII A'GATC_T
Cuts at: 0 542 1014
Size: 542 472
Bme1580I G_kGCm'C
Cuts at: 0 68 1014
Size: 68 946
BmrI ACTGGGnnnn_n'
Cuts at: 0 27 261 1014
Size: 27 234 753
Fragments arranged by size:
753 234 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 936 941 1014
Size: 559 377 5 73
Fragments arranged by size:
559 377 73 5
Bpu10I CC'TnA_GC
Cuts at: 0 703 1014
Size: 703 311
BsaI GGTCTCn'nnnn_
Cuts at: 0 554 1014
Size: 554 460
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 53 83 934 964 1014
Size: 53 30 851 30 50
Fragments arranged by size:
851 53 50 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 374 393 1014
Size: 374 19 621
Fragments arranged by size:
621 374 19
BseYI C'CCAG_C
Cuts at: 0 208 1014
Size: 208 806
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 1014
Size: 349 665
BsiHKAI G_wGCw'C
Cuts at: 0 960 1014
Size: 960 54
BsmI GAATG_Cn'
Cuts at: 0 586 1014
Size: 586 428
Bsp1286I G_dGCh'C
Cuts at: 0 68 960 1014
Size: 68 892 54
Fragments arranged by size:
892 68 54
BspHI T'CATG_A
Cuts at: 0 278 392 1014
Size: 278 114 622
Fragments arranged by size:
622 278 114
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 328 1014
Size: 328 686
BssSI C'ACGA_G
Cuts at: 0 236 1014
Size: 236 778
BstEII G'GTnAC_C
Cuts at: 0 165 1014
Size: 165 849
BstXI CCAn_nnnn'nTGG
Cuts at: 0 102 777 960 1014
Size: 102 675 183 54
Fragments arranged by size:
675 183 102 54
BstYI r'GATC_y
Cuts at: 0 542 1014
Size: 542 472
Bsu36I CC'TnA_GG
Cuts at: 0 142 1014
Size: 142 872
BtgI C'CryG_G
Cuts at: 0 728 1014
Size: 728 286
BtsI GCAGTG_nn'
Cuts at: 0 438 1014
Size: 438 576
EaeI y'GGCC_r
Cuts at: 0 869 1014
Size: 869 145
EarI CTCTTCn'nnn_
Cuts at: 0 55 1014
Size: 55 959
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 936 941 1014
Size: 559 377 5 73
Fragments arranged by size:
559 377 73 5
EcoO109I rG'GnC_Cy
Cuts at: 0 983 1014
Size: 983 31
EcoRI G'AATT_C
Cuts at: 0 736 1014
Size: 736 278
FspI TGC'GCA
Cuts at: 0 73 1014
Size: 73 941
HaeII r_GCGC'y
Cuts at: 0 137 1014
Size: 137 877
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 208 240 324 356 1014
Size: 208 32 84 32 658
Fragments arranged by size:
658 208 84 32 32
HindIII A'AGCT_T
Cuts at: 0 880 1014
Size: 880 134
MscI TGG'CCA
Cuts at: 0 871 1014
Size: 871 143
MslI CAynn'nnrTG
Cuts at: 0 415 1014
Size: 415 599
MspA1I CmG'CkG
Cuts at: 0 77 1014
Size: 77 937
NcoI C'CATG_G
Cuts at: 0 728 1014
Size: 728 286
NspI r_CATG'y
Cuts at: 0 813 1014
Size: 813 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 291 1014
Size: 291 723
PpuMI rG'GwC_Cy
Cuts at: 0 983 1014
Size: 983 31
PshAI GACnn'nnGTC
Cuts at: 0 308 492 1014
Size: 308 184 522
Fragments arranged by size:
522 308 184
PstI C_TGCA'G
Cuts at: 0 723 1014
Size: 723 291
RsrII CG'GwC_CG
Cuts at: 0 117 1014
Size: 117 897
SanDI GG'GwC_CC
Cuts at: 0 983 1014
Size: 983 31
SapI GCTCTTCn'nnn_
Cuts at: 0 55 1014
Size: 55 959
SexAI A'CCwGG_T
Cuts at: 0 760 1014
Size: 760 254
SfcI C'TryA_G
Cuts at: 0 719 1014
Size: 719 295
SmaI CCC'GGG
Cuts at: 0 172 358 1014
Size: 172 186 656
Fragments arranged by size:
656 186 172
StyI C'CwwG_G
Cuts at: 0 728 1014
Size: 728 286
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 1014
Size: 676 338
TatI w'GTAC_w
Cuts at: 0 269 1014
Size: 269 745
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 53 85 419 451 1014
Size: 53 32 334 32 563
Fragments arranged by size:
563 334 53 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 479 1014
Size: 479 535
XmaI C'CCGG_G
Cuts at: 0 170 356 1014
Size: 170 186 658
Fragments arranged by size:
658 186 170
Enzymes that do cut:
AarI AhdI ApoI AvaI BbsI BcgI BclI BglII
Bme1580I BmrI BpmI Bpu10I BsaI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BssSI BstEII
BstXI BstYI Bsu36I BtgI BtsI EaeI EarI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HindIII MscI MslI
MspA1I NcoI NspI PflMI PpuMI PshAI PstI RsrII
SanDI SapI SexAI SfcI SmaI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AleI AlfI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BciVI BfrBI BglI BlpI BmgBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstZ17I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspAI HincII
HpaI KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI PvuII SacI SacII SalI SbfI ScaI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI
ZraI