(Linear) (Six-base) MAPSORT of: KIBB4501.seq  Check: 7714  from: 1  to: 594



KIBB4501.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

ApoI r'AATT_y

Cuts at:      0     304     594 
   Size:        304     290
 
AvaI C'yCGr_G

Cuts at:      0     434     594 
   Size:        434     160
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     283     340     594 
   Size:        283      57     254

  Fragments arranged by size: 

                 283     254      57
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      89     123     594 
   Size:         89      34     471

  Fragments arranged by size: 

                 471      89      34
 
BglII A'GATC_T

Cuts at:      0     561     594 
   Size:        561      33
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     112     594 
   Size:        112     482
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     540     594 
   Size:        540      54
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     594 
   Size:        163     431
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     363     594 
   Size:        363     231
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     576     594 
   Size:        576      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     307     337     397     594 
   Size:        307      30      60     197

  Fragments arranged by size: 

                 307     197      60      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     418     594 
   Size:        418     176
 
BsmI GAATG_Cn'

Cuts at:      0     259     594 
   Size:        259     335
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     464     594 
   Size:        464     130
 
BssSI C'ACGA_G

Cuts at:      0     129     444     594 
   Size:        129     315     150

  Fragments arranged by size: 

                 315     150     129
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     265     594 
   Size:        265     329
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      40     594 
   Size:         40     554
 
BstYI r'GATC_y

Cuts at:      0     374     561     594 
   Size:        374     187      33

  Fragments arranged by size: 

                 374     187      33
 
Bsu36I CC'TnA_GG

Cuts at:      0      53     541     594 
   Size:         53     488      53

  Fragments arranged by size: 

                 488      53      53
 
BtgI C'CryG_G

Cuts at:      0     406     594 
   Size:        406     188
 
EaeI y'GGCC_r

Cuts at:      0      69     594 
   Size:         69     525
 
EcoRI G'AATT_C

Cuts at:      0     304     594 
   Size:        304     290
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      81     113     553     585     594 
   Size:         81      32     440      32       9

  Fragments arranged by size: 

                 440      81      32      32       9
 
HincII GTy'rAC

Cuts at:      0     344     594 
   Size:        344     250
 
MscI TGG'CCA

Cuts at:      0      71     594 
   Size:         71     523
 
MslI CAynn'nnrTG

Cuts at:      0     245     594 
   Size:        245     349
 
PstI C_TGCA'G

Cuts at:      0     461     594 
   Size:        461     133
 
SbfI CC_TGCA'GG

Cuts at:      0     461     594 
   Size:        461     133
 
SexAI A'CCwGG_T

Cuts at:      0     337     594 
   Size:        337     257
 
SfcI C'TryA_G

Cuts at:      0     457     594 
   Size:        457     137
 
SmlI C'TyrA_G

Cuts at:      0     178     594 
   Size:        178     416
 
StyI C'CwwG_G

Cuts at:      0     492     594 
   Size:        492     102
 
TatI w'GTAC_w

Cuts at:      0     501     554     594 
   Size:        501      53      40

  Fragments arranged by size: 

                 501      53      40
 
Tth111I GACn'n_nGTC

Cuts at:      0     339     594 
   Size:        339     255
 

 Enzymes that do cut:

     ApoI     AvaI     BbsI     BcgI    BglII     BmrI     BplI    BpuEI
     BsaI    BsaXI    BseRI     BsgI     BsmI    BspMI    BssSI   BstAPI
    BstXI    BstYI   Bsu36I     BtgI     EaeI    EcoRI    Hin4I   HincII
     MscI     MslI     PstI     SbfI    SexAI     SfcI     SmlI     StyI
     TatI  Tth111I

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI
 Bme1580I    BmgBI     BmtI     BpmI   Bpu10I    BsaAI    BsaBI    BsaHI
    BsaWI    BseYI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BstBI   BstEII
  BstZ17I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI